Immunoassay for the Determination of 7-Hydroxycoumarin in Serum Using 'Real-Time' Biosensor Analysis

Detection of microcystins in environmental samples using surface plasmon resonance biosensor

“…9000 RU) was observed by using (MC-LR)-BSA solution (100 g mL −1 ) in 10 mM sodium acetate (pH 4.3). Subsequently, the capacity of mAb binding at the 9000 RU-immobilization level was further checked by injecting the mAb solution (15 g mL −1 ) over the functional surface, yielding a 218 RU-capacity of mAb binding, similar to the results in the other developed inhibitive immunoassays: 80 RU-and 300 RU-capacities of the antibody binding were generated at the same high bioconjugate immobilization level (10,000 RU) [17,18]. Actually, in a certain immunoassay, the capacity value of antibody binding on the surface should be evaluated rationally based on the used antibody affinity, working concentration, and especially, the effective hapten immobilization level.…”

Production of a recombinant anti-morphine-3-glucuronide single-chain variable fragment (scFv) antibody for the development of a “real-time” biosensor-based immunoassay

“…However, previous assays developed in our laboratory to M3G using polyclonal antibodies show the ELISA to provide lower detection limits compared to the BIAcore biosensor (Dillon et al, 2003) indicating the advantage of the scFv for analysis on the BIAcore biosensor. Furthermore, when comparing the M3G assay developed in this paper to previous assays developed to molecules such as comarin (Keating et al, 1999) and aflatoxin B 1 (Daly et al, 2000), a comparison of detection limits has proved favourable. Clearly, in both the ELISA and biosensor formats, the scFv produced is very sensitive and has low limits of detection in ELISA while also being suitable for use in biosensor applications.…”

Survey of the 1999 surface plasmon resonance biosensor literature

“…7 Examples of smallmolecule SPR analysis include immobilizing these compounds on the flow cell surface and following their interactions with macromolecules in solution 396,413 or using the compounds as soluble binding competititors. 395,398,400,402,405,406,409,411,412,414,415 A number of papers describe the application of BIACORE in the direct detection of soluble small molecules binding immobilized macromolecules. 397,399,401,403,404,407,408,410 The ability to collect high-resolution kinetic information on drug-target interactions is making SPR a complementary tool to technologies currently employed in the high-throughput screening and lead optimization phases of pharmaceutical discovery.…”