Immunoassay of cooked wild rat meat by ELISA with a highly specific antibody targeting rat heat-resistant proteins

Chen, Xiangmei; Ran, Di; Zeng, Lin; Xin, Meiguo

doi:10.1080/09540105.2020.1740180

Cited by 10 publications

(5 citation statements)

References 29 publications

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“…Nowadays, proteins are commonly detected by the ELISA (Ahmad & Cheema, 2019;Chen et al, 2020;Seo & Joo, 2014;Walschus et al, 2002). Bt protein content was determined by the ELISA method, using the AP003 ELISA quantitative kit from EnviroLogix, Inc.…”

Section: Determination Of Bt Protein Contentmentioning

confidence: 99%

Spatial and temporal distribution of Bt proteins in Bt maize leaves

Nie

Wang

Luo

et al. 2021

Food and Agricultural Immunology

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To learn the spatial and temporal distribution of Bt proteins in Bt maize leaves, Bt protein levels of Bt maize leaves and their corresponding non-transgenic lines were detected and analysed by the ELISA method. The results showed that Bt proteins were expressed in the leaves of Bt maize varieties during all periods of their growth, while no such Bt proteins were detected in the leaves of non-transgenic maize varieties. The range of leaf Bt proteins was 1.08∼5.81 µg.g −1 FW, which could effectively protect Bt maizes from the corn borer pest during their growth periods. Furthermore, the younger leaves of Bt maizes had significantly lower levels of Bt proteins than their older leaves. In most periods, Bt protein content of Bt maize leaves under drought stress was lower than that under normal growth conditions, but Bt protein content under nutrition stress was higher than that under normal growth conditions. The results provided a full pattern of the spatial and temporal distribution of Bt proteins in Bt maize leaves.

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Section: Determination Of Bt Protein Contentmentioning

confidence: 99%

Spatial and temporal distribution of Bt proteins in Bt maize leaves

Nie

Wang

Luo

et al. 2021

Food and Agricultural Immunology

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“…Penelitian terhadap sensitivitas spesies anjing dan tikus menggunakan primer gen ATPase 6 serta ND5 untuk spesies babi memiliki sensitivitas 0.01 hingga 0.02 ng (Ali et al, 2015). Sensitivitas pada spesies tikus diuji menggunakan metode sandwich ELISA imunogen dan antibodi dengan limit deteksi 0,01 µg/L (Chen et al, 2020).…”

Section: Simplex-dan Multiplex-pcrunclassified

“…Penelitian ini apabila dibandingkan dengan penelitian terdahulu, primer gen 12S rRNA lebih sensitif (Matsunaga et al, 1999;Dalmasso et al, 2004;Yin et al, 2009;Ali et al, 2015;Hossain et al, 2017;Iwobi et al, 2017;Prusakova et al, 2018;Liu et al, 2019;Li et al, 2019). Primer gen 12S rRNA untuk spesies babi sama sensitif dengan primer gen COI yaitu 0,001 ng (Wang et al, 2019), kurang sensitif untuk spesies babi apabila dibandingkan dengan primer cyt b oleh Lubis et al (2018), dan kurang sensitif untuk spesies tikus oleh Chen et al (2020).…”

Section: Simplex-dan Multiplex-pcrunclassified

Sensitivitas Multiplex-Polymerase Chain Reaction Gen 12S rRNA dalam Mendeteksi Pemalsuan Daging Sapi dengan Daging Babi, Anjing dan Tikus

et al. 2021

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Daging sapi dan olahannya merupakan sasaran pemalsuan. Pelaku pemalsuan daging sapi mencampur daging sapi dengan daging spesies hewan lain, sehingga berakibat pada berubahnya status kehalalan daging. Selain itu, kondisi tersebut dapat menjadi penyebab serius pada risiko kesehatan, seperti potensi keracunan, sumber penyakit maupun alergi. Pengawasan yang efektif diperlukan guna menjamin keaslian dan keamanan daging. Metode deteksi secara molekuler yang umum digunakan adalah multiplex-PCR karena efisien dan sensitif. Tujuan penelitian ini untuk mendeteksi sensitivitas multiplex-PCR primer gen 12S rRNA yang spesifik untuk spesies sapi, anjing, babi, dan tikus. Template DNA dibuat masing-masing enam konsentrasi, yaitu 25; 10; 1; 0,1; 0,01; 0,001 ng/ìL untuk proses simplex dan multiplex-PCR. Hasil uji sensitivitas menggunakan metode simplex-PCR menunjukkan bahwa primer gen 12S rRNA dapat mengamplifikasi spesies sapi, anjing, babi, dan tikus dengan akurat hingga konsentrasi 0,001 ng/ìL. Pengujian sensitivitas multiplex-PCR gen 12S rRNA dapat mengamplifikasi spesies sapi, anjing, babi hingga konsentrasi 0,001 ng/ìL dan pada spesies tikus hingga konsentrasi 1 ng/ìL. Metode PCR ini dapat digunakan sebagai solusi alternatif dalam studi halal dan verifikasi label pangan

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“…Fourier Transform Infrared Spectroscopy is the most commonly used technique. [11], Real-Time Polymerase Chain Reaction [12], multiplex PCR [13], and ELISA [14]. Another method that can be developed in analyzing adulteration containing animal meat, especially non-halal meat (rat meat), is to look at the fatty acid composition contained in it.…”

Section: Introductionmentioning

confidence: 99%

Authentication of Rattus Norvegicus Fat and Other Animal Fats Using Gas Chromatography-Mass Spectrometry (Gc-Ms) and Principal Component Analysis (Pca)

et al. 2023

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Objective: The objective of this study was to analyze fatty acids using Gas Chromatography-Mass Spectrometry (GC-MS) in combination with chemometric Principal Component Analysis (PCA) for the authentication of Rattus norvegicus fat from other animal fats. Methods: Extraction of fat from raw meat of Rattus norvegicus, beef, chicken, pork, and dogs using the Bligh Dyer method, then derivatized with 0.2 N NaOCH3, precipitation of sodium glycerol was carried out by adding saturated NaCl to obtain methyl esters which were then injected into the GC-MS instrument. The GC-MS data were then processed using chemometric Principal Component Analysis (PCA) to group Rattus norvegicus fat with other animal fats (beef, chicken, pork, and dog). Results: The results of the study revealed that fatty acids in Rattus norvegicus using GC-MS produced eleven types of fatty acids, namely: Lauric acid (1,1%), Myristic acid (1,15%), Palmitic acid (21,12%), Palmitoleic acid (2,06%), Stearic acid (8,23%), Vaccenic acid (2,43%), Oleic acid (26,51%), Linoleic acid (19,19%), Arachidic acid (0,09%), and Eucosatrienoic acid (0,39%). Chemometrics Principal Component Analysis (PCA) of Rattus norvegicus fat allows it to be classified with other animal fats. Conclusion: The Gas Chromatography-Mass Spectrometry (GC-MS) method, in combination with chemometric Principal Component Analysis (PCA), offered effective tools for the authentication of fatty acid of Rattus norvegicus.

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Immunoassay of cooked wild rat meat by ELISA with a highly specific antibody targeting rat heat-resistant proteins

Cited by 10 publications

References 29 publications

Spatial and temporal distribution of Bt proteins in Bt maize leaves

Spatial and temporal distribution of Bt proteins in Bt maize leaves

Sensitivitas Multiplex-Polymerase Chain Reaction Gen 12S rRNA dalam Mendeteksi Pemalsuan Daging Sapi dengan Daging Babi, Anjing dan Tikus

Authentication of Rattus Norvegicus Fat and Other Animal Fats Using Gas Chromatography-Mass Spectrometry (Gc-Ms) and Principal Component Analysis (Pca)

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