Immunoassay of insulin with insulin-antibody precipitate

Abstract

Micromethods for the assay of insulin based on the reaction between 131I-labelled insulin and insulin antibody have been described by Berson (1960) and Grodsky & Forsham (1960). In

Activation by glucose of adenyl cyclase in pancreatic islets of the rat

“…Samples were withdrawn and assayed for insulin content using a double-antibody radioimmunoassay [9] with rat insulin as standard.…”

2‐5A‐dependent endoribonuclease activity and effects of A2'p5'A2'p5'A (2‐5A core) in mouse pancreatic islets

“…The islets were then incubated a second time at 37°C between 15-210 min. After this incubation the islets were centrifuged down and the supernatant removed for assay of insulin released by radioimmunoassay [23]. (Residual t3H]leucine present did not significantly interfere with this 70 assay.)…”

“…No attempt was made to distinguish between proinsulin and msulin. Total insulin content [23] and DNA content [25] were also determined in the somcate. Results are expressed in terms of islet DNA.…”

Inhibition by somatostatin of glucose induced 3′: 5′‐monophosphate (Cyclic AMP) accumulation and insulin release in isolated pancreatic islets of the rat

“…[3H]cyclic AMP was purified by ion-exchange chromatography and barium sulfate precipitation [7]. Insulin was measured radioimmunologically using a double antibody system [8].…”