Immunoassay of prostate-specific antigen (PSA) using resonant frequency shift of piezoelectric nanomechanical microcantilever

Lee, Jeong Hoon; Hwang, Kyo Seon; Park, Jaebum; Yoon, Ki Hyun; Yoon, Dae Sung; Kim, Tae Song

doi:10.1016/j.bios.2004.09.024

Cited by 188 publications

(72 citation statements)

References 15 publications

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“…The reported benefits of using nanocantilever systems in cancer detection include that there are no requirements for fluorescent or radioactive labeling; detection may take place in liquid samples and this technology may be easily translated to lab-on-a-chip techniques, providing point-of-care diagnostics (16). They have also been described as a simple replacement to polymerase chain reaction reactions and detection methods, as they are more cost-effective regarding sample preparation, in terms of time and costly materials.…”

Section: Cantileversmentioning

confidence: 99%

Advances in the application of nanotechnology in the diagnosis and treatment of gastrointestinal tumors

Sun

Fang

et al. 2014

Molecular and Clinical Oncology

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Abstract. Nanotechnology has broad application prospects in the diagnosis and treatment of cancer. Integrating chemistry, engineering, biology and medicine, nanotechnology is a multidisciplinary research field. Nanoscale imaging technology significantly improves the precision and accuracy of tumor diagnosis. Nanocarriers are able to significantly improve the accuracy of dose and targeted drug delivery and reduce the toxic side effects. This review focuses on the emerging roles of these innovative technologies in gastrointestinal cancer diagnostics and therapeutics. Although several problems and barriers are hampering the development of nanodevices, the potential for nanotechnologies to function as multimodal nanotheranostic agents will likely pave the way for the fight against gastrointestinal cancer.

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Section: Cantileversmentioning

confidence: 99%

Advances in the application of nanotechnology in the diagnosis and treatment of gastrointestinal tumors

Sun

Fang

et al. 2014

Molecular and Clinical Oncology

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“…This is by far the most sensitive method, even if significantly more complex than that for deflection-based detection [122]. Using this more sensitive resonancebased measurement strategy, PSA with an LOD of 10 pg mL -1 was detected [128]. The incorporation of piezoelectric materials into microcantilevers can also be used to monitor the presence of biomolecules.…”

Section: Micro-cantilever Immunosensorsmentioning

confidence: 99%

“…Analyte binding to the cantilever surface causes the cantilever to deflect, consequently depolarising the material and generating a current. A piezoelectric nanomechanical cantilever was fabricated by Lee et al for the label free detection of PSA with an LOD of 10 pg mL -1 [130].…”

Section: Micro-cantilever Immunosensorsmentioning

confidence: 99%

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Trends and Perspectives in Immunosensors

2012

Antibodies Applications and New Developments

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Immunosensors are devices that comprise both a biomolecular recognition system, such as an antibody and its corresponding antigen, and a transducer to translate the high affinity and specific binding event into a physical signal.Antibodies are produced by an immunological response to the presence of a foreign substance called an antigen. Antibodies may be immobilised onto a variety of platforms including bulk planar surfaces and nanoparticles by either covalent or adsorption strategies. Different interfaces between the bio-components and the detector are available to monitor in 'real-time' the signal generated by biological interactions. The transducers detect, for example, the change in electron transfer, absorbance, fluorescence, refractive index, mass change or heat transfer as the antibody binds to the antigen of interest. Such analytical devices have allowed a wide range of analytes to be identified and quantified such as pathogens, toxins, environmental food contaminants and disease biomarkers.The demand for sensitive, rapid, 'on-site' techniques has taken advantage of the latest advances in microfluidics and nanotechnology. This chapter will highlight current trends in immobilisation, micro/nano-fluidics/ and transducers utilised. A number of examples outlining the exploitation of these elements in immunosensors and their successful applications will be described.

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“…When a cantilever biosensor operates in static mode, the deflection resulted from the surface stress of the adsorbed biomaterials has been measured by optical measurement system [6,7], which is so bulky and expensive that it is almost impossible to minimize the device size for a Lab-On-a-Chip (LOC) system. There are other detection limitations such as a narrow dynamic range and parasitic deflection in optical measurements [8].…”

Section: Introductionmentioning

confidence: 99%