Immunoassay or LC-MS/MS for the measurement of salivary cortisol in children?

Bae, Yoon Ju; Gaudl, Alexander; Jaeger, Sonia; Stadelmann, Stephanie; Hiemisch, Andreas; Kieß, Wieland; Willenberg, Anja; Schaab, Michael; Klitzing, Kai von; Thiery, Joachim; Ceglarek, Uta; Döhnert, Mirko; Kratzsch, Juergen

doi:10.1515/cclm-2015-0412

Cited by 44 publications

(25 citation statements)

References 36 publications

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“…In order to achieve this aim, future studies should rigorously verify the self-reported awakening times (e.g., by actigraphy) and sampling times using electronic monitoring devices (e.g., MEMS® caps). Second, we converted salivary cortisol concentrations to adjust for measurement bias due to antibody cross-reactivity and steric effects of further saliva matrix components (Miller et al, 2013; Bae et al, 2016). Therefore, the provided reference ranges only apply to salivary cortisol as measured by a compatible LC/MS-MS protocol and would need to be adjusted whenever cortisol data from other assays are compared with our values and ranges.…”

Section: Discussionmentioning

confidence: 99%

“…These assays were often adopted for saliva analysis without any further adjustments, which entails large between-assay variability and the overestimation of salivary cortisol at low concentrations as compared to unbiased mass spectrometric methods (Miller et al, 2013; Bae et al, 2016). These measurement peculiarities become particularly problematic for research designs that lack internal control groups and would therefore need to rely on reference values that have been derived using incompatible assays.…”

Section: Introductionmentioning

confidence: 99%

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The CIRCORT database: Reference ranges and seasonal changes in diurnal salivary cortisol derived from a meta-dataset comprised of 15 field studies

Miller

Stalder

Jarczok³

et al. 2016

Psychoneuroendocrinology

186

121

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Diurnal salivary cortisol profiles are valuable indicators of adrenocortical functioning in epidemiological research and clinical practice. However, normative reference values derived from a large number of participants and across a wide age range are still missing. To fill this gap, data were compiled from 15 independently conducted field studies with a total of 104,623 salivary cortisol samples obtained from 18,698 unselected individuals (mean age: 48.3 years, age range: 0.5 to 98.5 years, 39% females). Besides providing a descriptive analysis of the complete dataset, we also performed mixed-effects growth curve modeling of diurnal salivary cortisol (i.e., 1 to 16 hours after awakening). Cortisol decreased significantly across the day and was influenced by both, age and sex. Intriguingly, we also found a pronounced impact of sampling season with elevated diurnal cortisol in spring and decreased levels in autumn. However, the majority of variance was accounted for by between-participant and between-study variance components. Based on these analyses, reference ranges (LC/MS-MS calibrated) for cortisol concentrations in saliva were derived for different times across the day, with more specific reference ranges generated for males and females in different age categories. This integrative summary provides important reference values on salivary cortisol to aid basic scientists and clinicians in interpreting deviations from the normal diurnal cycle.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

The CIRCORT database: Reference ranges and seasonal changes in diurnal salivary cortisol derived from a meta-dataset comprised of 15 field studies

Miller

Stalder

Jarczok³

et al. 2016

Psychoneuroendocrinology

186

121

View full text Add to dashboard Cite

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“…Noteworthy, the methods for collecting salivary cortisol, testing and analyzing cortisol concentration levels have improved in more recent years. These include immunoassay or liquid chromatography-tandem mass spectrometry (LC-MS/MS) for the measurement of salivary cortisol [ 37 , 38 ]. Therefore, there is need to update literature to include these new methods when investigating the relationship between cortisol concentration levels and PTSD.…”

Section: Discussionmentioning

confidence: 99%

Salivary cortisol in post-traumatic stress disorder: a systematic review and meta-analysis

et al. 2018

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BackgroundStudies investigating salivary cortisol level as susceptibility marker for post-traumatic stress disorder (PTSD) produced inconsistent results. The aim of this study was to compare salivary cortisol concentration levels in PTSD patients with those in controls by synthesizing published data.MethodsWe did a systematic review, meta-analysis and meta-regression of studies comparing concentrations of salivary cortisol between patients with PTSD and controls. The electronic databases of PubMed, Embase, Web of Science and Psyc-ARTICLES were searched for relevant articles. A random-effects model with restricted maximum-likelihood estimator is used to synthesize the effect size (assessed by standardized mean difference).ResultsA total of 784 articles were identified of which 22 were included in the final analysis. A trend of lower salivary cortisol levels was found in PTSD patients when compared with the controls (SMD = − 0.28, 95% CI-0.53;-0.04, p = 0.022). Subgroup analysis showed that the salivary cortisol levels were lower in patients with PTSD than in controls in studies conducted after 2007 or in studies using saliva samples collected in the morning.ConclusionsThe evidence from this meta-analysis supports that salivary samples collected in the morning consistently showed a lower salivary cortisol level in patients with PTSD than in controls, although whether salivary cortisol could be used as a diagnostic tool requires further research.Electronic supplementary materialThe online version of this article (10.1186/s12888-018-1910-9) contains supplementary material, which is available to authorized users.

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“…Additionally, saliva cortisol concentration was measured using ELISA. Although, studies found that immunoassay analysis of saliva cortisol concentration largely comparable to liquid chromatography tandem mass spectrometry (LC-MS/MS), immunoassay values were consistently greater than LC-MS/MS and the limit of detection was 5 nmol/L (0.18 μg/dL) or more [55]. Despite limitations, these data support the need for further investigations into the interactions among circadian rhythms of cortisol with rest-activity rhythms, sleep metrics and maternal-fetal health.…”

Section: Considerations Of Study Population and Study Limitationsmentioning

confidence: 99%

Pregnancy rest-activity patterns are related to salivary cortisol rhythms and maternal-fetal health indicators in women from a disadvantaged population

et al. 2020

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Irregular rest-activity patterns can disrupt metabolic and hormonal physiology and potentially lead to disease. Little is known regarding rest-activity patterns during gestation and their association with hormonal rhythms and health in pregnant women. We conducted a pilot study to determine if 24 h rest-activity was related to saliva cortisol rhythms and maternal-fetal health in an economically disadvantaged population. Primiparous women wore a wrist actigraphy device for a week to record activity during gestational weeks 22 (G22; n = 50) and 32 (G32; n = 46) and postpartum week one (PPW1; n = 39). Participants collected saliva samples every 4 hr over a 24 hr period during G22 (n = 22), G32 (n = 20) and 24-48 hr postnatal (n = 20), and cortisol concentrations were measured with ELISA. Circadian rhythmicity was assessed using autocorrelation coefficient (r24) and cosinor analysis. Blood glucose levels, body mass index (BMI), gestational disease data, and gestational age of infant at birth were abstracted from medical charts. Time of cortisol peak (acrophase) during G22 was related with acrophase of activity (r = 0.66; p = 0.001) and blood glucose levels (r = 0.58; p = 0.006). During G22, minutes of wake after sleep onset was positively related to cortisol mesor and AUC (p <0.05). Rest-activity r24, R 2 , and mesor during G32 were positively (p<0.05) associated with gestational age of infant at birth. Across all three time points r24 of activity was related with cortisol amplitude (r = 0.33; p = 0.01). Findings support a relationship between rest-activity patterns and saliva cortisol rhythms during pregnancy. The association of less robust activity rhythms with earlier gestational age of infant at birth indicates a potential link between circadian system disruption and maternal-fetal health outcomes.

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Immunoassay or LC-MS/MS for the measurement of salivary cortisol in children?

Abstract: Immunoassay and LC-MS/MS were largely comparable in the interpretation of salivary cortisol dynamics in stress research. But the IA method revealed a restricted accuracy in the measuring range below 5 nmol/L.

Cited by 44 publications

References 36 publications

The CIRCORT database: Reference ranges and seasonal changes in diurnal salivary cortisol derived from a meta-dataset comprised of 15 field studies

The CIRCORT database: Reference ranges and seasonal changes in diurnal salivary cortisol derived from a meta-dataset comprised of 15 field studies

Salivary cortisol in post-traumatic stress disorder: a systematic review and meta-analysis

Pregnancy rest-activity patterns are related to salivary cortisol rhythms and maternal-fetal health indicators in women from a disadvantaged population

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