Immunoassay Techniques Highlighting Biomarkers in Immunogenetic Diseases

Manole, Emilia; Bastian, Alexandra; Popescu, Ionela Daniela; Constantin, Carolina; Mihai, Simona; Găină, Gisela; Codrici, Elena; Neagu, Monica

doi:10.5772/intechopen.75951

Cited by 13 publications

(9 citation statements)

References 125 publications

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“…ELISA usually uses coated wells with recombinant proteins or linear peptides produced in transfected bacteria (e.g. E. coli) or baculovirus-infected insect cells (Engvall and Perlmann, 1972;Possee, 1997;Manole et al, 2018).…”

Section: Enzyme-linked Immunosorbent Assaymentioning

confidence: 99%

Detection of autoantibodies in central nervous system inflammatory disorders: Clinical application of cell-based assays

Molina

Conzatti

Silva

et al. 2020

Multiple Sclerosis and Related Disorders

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Section: Enzyme-linked Immunosorbent Assaymentioning

confidence: 99%

Detection of autoantibodies in central nervous system inflammatory disorders: Clinical application of cell-based assays

Molina

Conzatti

Silva

et al. 2020

Multiple Sclerosis and Related Disorders

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“…Various studies have suggested biomarkers for liver cancer, and the effort to identify additional markers is ongoing ( 40 ). Numerous methods, including immunohistochemistry, ELISA and western blotting, have previously been used in such studies ( 41 , 42 ). However, these methods are relatively time-consuming and expensive.…”

Section: Discussionmentioning

confidence: 99%

HMBS is the most suitable reference gene for RT‑qPCR in human HCC tissues and blood samples

et al. 2021

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Reverse transcription-quantitative (RT-q) PCR is the most feasible and useful technique for identifying and evaluating cancer biomarkers; however, the method requires suitable reference genes for gene expression analysis. The aim of the present study was to identify the most suitable reference gene for the normalization of relative gene expression in human hepatocellular carcinoma (HCC) tissue and blood samples. First, 14 candidate reference genes were selected through a systematic literature search. The expression levels of these genes ( ACTB, B2M, GAPDH, GUSB, HMBS, HPRT1, PGK1, PPIA, RPLP0, RPL13A, SDHA, TBP, TFRC and YWHAZ ) were evaluated using human multistage HCC transcriptome data (dataset GSE114564), which included normal liver (n=15), chronic hepatitis (n=20), liver cirrhosis (n=10), and early (n=18) and advanced HCC (n=45). From the 14 selected genes, five genes, whose expression levels were stable in all liver disease statuses ( ACTB, GAPDH, HMBS, PPIA and RPLP0 ), were further assessed using RT-qPCR in 40 tissues (20 paired healthy tissues and 20 tissues from patients with HCC) and 40 blood samples (20 healthy controls and 20 samples from patients with HCC). BestKeeper statistical algorithms were used to identify the most stable reference genes, of which HMBS was found to be the most stable in both HCC tissues and blood samples. Therefore, the results of the present study suggest HMBS as a promising reference gene for the normalization of relative RT-qPCR techniques in HCC-related studies.

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“…Singleplex immunoassays (i.e. an assay with only one target) are the primary clinical format used at present 70 . While many diseases can rely on single analyte analysis for optimal patient outcomes (e.g.…”

Section: 24 Immunoassay Limitationsmentioning

confidence: 99%

Facilitating serum determination of neuron specific enolase at clinically relevant levels by coupling on-line molecularly imprinted solid-phase extraction to LC-MS/MS

McKitterick

Bicak

Cormack

et al. 2020

Analytica Chimica Acta

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First and foremost, I must thank my supervisors Trine Grønhaug Halvorsen and Leon Reubsaet for helping me every step of the way and helping me to grow as a person and a scientist. I am especially grateful for their patience, understanding, and optimism. I am proud to be their Ph.D. student. I would be remiss not to thank my amazing collaborators Peter Cormack and Cem Biçak from the University of Strathclyde, Glasgow. Thank you for producing the materials used in this Ph.D. and for helping me to understand the complexities of imprinted polymers. To my masters' students, Frida Braathen and Jasna Petrovic, thank you for your invaluable contributions to this work. To all my colleagues throughout the years, thank you all so much for the amazing work environment, chats, and good times. You helped to create a wonderful work environment. I would like to especially thank Magnus Restan for helping me adjust to life in Norway and always being available for chats about life and football. To my family, I am thankful for your support and encouragement. I know it has been difficult with me being so far from home. Especially I'd like to thank Mark for sacrificing his Saturday mornings and helping with the graphical abstracts. Most of all I would like to thank my Zhenya. You have been amazing these last few years. Thank you so much for your love and support.

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Immunoassay Techniques Highlighting Biomarkers in Immunogenetic Diseases

Cited by 13 publications

References 125 publications

Detection of autoantibodies in central nervous system inflammatory disorders: Clinical application of cell-based assays

Detection of autoantibodies in central nervous system inflammatory disorders: Clinical application of cell-based assays

HMBS is the most suitable reference gene for RT‑qPCR in human HCC tissues and blood samples

Facilitating serum determination of neuron specific enolase at clinically relevant levels by coupling on-line molecularly imprinted solid-phase extraction to LC-MS/MS

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