Immunobiosensor Detection of Domoic Acid as a Screening Test in Bivalve Molluscs: Comparison with Liquid Chromatography-Based Analysis

Abstract

A rapid and sensitive immuno-based screening method was developed to detect domoic acid (DA) present in extracts of shellfish species using a surface plasmon resonance-based optical biosensor. A rabbit polyclonal antibody raised against DA was mixed with standard or sample extracts and allowed to interact with DA immobilized onto a sensor chip surface. The characterization of the antibody strongly suggested high cross-reactivity with DA and important isomers of the toxin. The binding of this antibody to the sensor chip surface was inhibited in the presence of DA in either standard solutions or sample extracts. The DA chip surface proved to be highly stable, achieving approximately 800 analyses per chip without any loss of surface activity. A single analytical cycle (sample injection, chip regeneration, and system wash) took 10 min to complete. Sample analysis (scallops, mussels, cockles, oysters) was achieved by simple extraction with methanol. These extracts were then filtered and diluted before analysis. Detection limits in the ng/g range were achieved by the assay; however, the assay parameters chosen allowed the test to be performed most accurately at the Euopean Union's official action limit for DA of 20 g/g. At this concentration, intra- and interassay variations were measured for a range of shellfish species and ranged from 4.5 to 7.4% and 2.3 to 9.7%, respectively.

Fluorescent microarray for multiplexed quantification of environmental contaminants in seawater samples

“…Irgarol 1051®) [28,29,37], sulfonamide [18,30], chloramphenicol, PBDEs (i.e. BDE-47) [38], and domoic acid [33]. Dependent on the class specificity shown by some of these immunoreagents (i.e >10 sulfonamide antibiotic congeners are recognized by the immunoreagents used in this work), a microarray platform able to detect a large number of chemical substances [33] can potentially be developed.…”

Survey of the year 2006 commercial optical biosensor literature

“…(27%), X (14%), and 1000 (5%) platforms, with a few investigators describing work performed using Bialite [810], C [1016], J [205,323,325,326,696,752], Q [771,1025,1026,1031,1034,1036,1037], S51 [194,395,591,828,887,902,917,919,929,932,933,937], and Flexchip [135,494,665,1059]. Also, in 2006 several articles describing Biacore's recently released T100 [363,435,629,695,719,929,937] and A100 [295,455] platforms were published.…”

“…The technology's breadth of applications is further illustrated by the range of articles investigating oligonucleotides , small molecules , carbohydrates , and extracellular matrix components . In addition, the number of articles describing biosensor work in clinical support [1008][1009][1010][1011][1012][1013][1014][1015][1016][1017][1018][1019][1020][1021][1022][1023][1024], food/agricultural/veterinary/environmental sciences [1025][1026][1027][1028][1029][1030][1031][1032][1033][1034][1035][1036][1037][1038], and membrane/virus/cell characterization [1039][1040][1041][1042][1043][1044][1045][1046][1047][1048][1049][1050] demonstrate Biacore's emerging contributions in these fields. Biacore's more unusual applications, including the characterization of fibrils and the analysis of crude analytes are summarized in References 1051-1066.…”

A surface plasmon resonance system for the underwater detection of domoic acid

“…Since DA is a small molecule of only 311 Da and the SPR signal shift is proportional to added quantities of matter, a direct detection would not be sensitive enough to detect the toxin at concentration lower than few ng mL 21 (Lotierzo et al 2004;Yu et al 2005;Le Berre and Kane 2006;Traynor et al 2006;Stevens et al 2007;Doucette et al 2009;Campbell et al 2011). To circumvent this limitation, an alternative method relies on an indirect detection such as an inhibition assay format (Dost alek et al 2006).…”

“…However, no in situ SPR biosensor has been deployed to date. In contrast, numerous quantitative laboratory-based SPR detection systems have been developed for various marine molecules in particular the biotoxin domoic acid (DA) (Lotierzo et al 2004;Yu et al 2005;Le Berre and Kane 2006;Traynor et al 2006;Stevens et al 2007;Doucette et al 2009;Campbell et al 2011). Currently, the most sensitive laboratory SPR assay can detect DA as low as 0.1 lg L 21 in a shellfish extract (Yu et al 2005).…”