Immunochemical Analysis of Antigenic Determinants of Chlamydia trachomatis by Monoclonal Antibodies

Matikainen, M; Terho, P

doi:10.1099/00221287-129-8-2343

Cited by 8 publications

(7 citation statements)

References 12 publications

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“…Salari and Ward were able to extract MOMP from 14 of the 15 then-known serovars (see below) of the chlamydial species Chlamydia trachomatis and noted minor serovar-specific variations in its molecular weight (183). The existence of species-and subspecies-specific epitopes within the protein was noted (37,206), before it became clear that MOMP also contained serovar-specific epitopes (138,162). Even more important, polyclonal (36) and then monoclonal (168,251) antibodies to MOMP were shown to neutralize infectivity of the live organism.…”

Section: Structurementioning

confidence: 99%

Diagnosis and Assessment of Trachoma

et al. 2004

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Trachoma is caused by Chlamydia trachomatis. Clinical grading with the WHO simplified system can be highly repeatable provided graders are adequately trained and standardized. At the community level, rapid assessments are useful for confirming the absence of trachoma but do not determine the magnitude of the problem in communities where trachoma is present. New rapid assessment protocols incorporating techniques for obtaining representative population samples (without census preparation) may give better estimates of the prevalence of clinical trachoma. Clinical findings do not necessarily indicate the presence or absence of C. trachomatis infection, particularly as disease prevalence falls. The prevalence of ocular C. trachomatis infection (at the community level) is important because it is infection that is targeted when antibiotics are distributed in trachoma control campaigns. Methods to estimate infection prevalence are required. While culture is a sensitive test for the presence of viable organisms and nucleic acid amplification tests are sensitive and specific tools for the presence of chlamydial nucleic acids, the commercial assays presently available are all too expensive, too complex, or too unreliable for use in national programs. There is an urgent need for a rapid, reliable test for C. trachomatis to assist in measuring progress towards the elimination of trachoma

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Section: Structurementioning

confidence: 99%

Diagnosis and Assessment of Trachoma

et al. 2004

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“…Examples of observed specificities include genus-; species-; broad subspecies-, as in the B-and C-complex groups; narrow subspecies-, as in the close relationship of serovars E and D (22); and serovar-specific reactions. The molecular basis for such antigenic interrelationships has remained unknown, but recent studies have implicated chlamydial lipopolysaccharide (3) and surface-exposed outer membrane proteins (5,11,20).…”

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confidence: 99%

Antigenic analysis of the major outer membrane protein of Chlamydia trachomatis with murine monoclonal antibodies

Batteiger¹,

Th²,

Terho³

et al. 1986

Infect Immun

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We prepared monoclonal antibodies against prototype strains of the 15 serovars of Chiamydia trachomatis and identified a subset of reagents that reacted with the major outer membrane protein(s) (MOMPs) of one or more serovars. We then determined the specificities of these anti-MOMP monoclonal antibodies by radioimmunoassay and immunoblot assays against the 15 serovars of C. trachomatis and a C. psittaci strain. We identified 14 different anti-MOMP antibody specificities, including serovar-, several orders of subspecies-, and species-specific determinants. In addition, one antibody reacted with all C. trachomatis serovars and a C. psittaci strain, indicating the presence of a genus-specific epitope on MOMP. Many of the cross-reactions of the subspecies-specific antibodies were similar to those previously reported by use of the microimmunofluorescence technique. We also observed a number of cross-reactions that were unexpected but consistent with data derived by the microimmunofluorescence test. All antibodies, except the genus-specific antibodies, reacted with whole elementary bodies in a radioimmunoassay, suggesting surface exposure of the epitopes. These data confirm and extend previous observations that MOMPs among C. trachomatis serovars are antigenically complex and diverse. In addition, these data indicate that the cross-reaction patterns of some monoclonal antibodies directed against MOMP are similar to those detected by the microimmunofluorescence test and are consistent with the hypothesis that such determinants are contained within MOMPs.

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“…Stephens et al (19) reported the presence of species-and subspecies-specific determinants in MOMPs, and Matikainen and Terho first showed that MOMPs contain serovar-specific determinants. (20) In addition, several researchers confirmed that anti-MOMP MAbs of C. trachomatis play an important role in identifying the presence of genus-, species-, serovar-, and diverse subspecies-specific determinants in MOMPs. (21) In this study, we compared the DFA test for detecting C. trachomatis infections with a newly developed IFA test.…”

Section: Discussionmentioning

confidence: 96%

Evaluation of a Developed Species-Specific Monoclonal Antibody for DetectingChlamydia trachomatisInfections in Endocervical Specimens from Female Patients

Kumar

Singh²,

Salhan³

et al. 2007

Hybridoma

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To increase the sensitivity and reliability of immunodiagnostic assay for direct detection of chlamydial genital infection, we developed monoclonal antibodies (MAbs) that recognize the major outer membrane protein (MOMP) of all serovars of Chlamydia trachomatis. ELISA and dot-ELISA were used to select hybridomas that produced anti-C. trachomatis MAbs. Four MAb clones--A12.6, B serogroup specific; C1.5, C serogroup specific; G10.2, species specific; and E9.1, genus specific--were characterized by SDS-PAGE and immunoblot and were reactive with MOMP of C. trachomatis. The species-specific MAb G10.2 had a molecular mass of 40 kDa. The reactivity of developed species-specific monoclonal antibody for detection of C. trachomatis antigen in endocervical specimens was compared with the commercially available DFA test. We found that developed antibody had high sensitivity (97.22%) compared to DFA for detection of chlamydial infection in endocervical samples. It can be used as a reliable method in laboratories for the diagnosis of chlamydial infections.

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Immunochemical Analysis of Antigenic Determinants of Chlamydia trachomatis by Monoclonal Antibodies

Cited by 8 publications

References 12 publications

Diagnosis and Assessment of Trachoma

Diagnosis and Assessment of Trachoma

Antigenic analysis of the major outer membrane protein of Chlamydia trachomatis with murine monoclonal antibodies

Evaluation of a Developed Species-Specific Monoclonal Antibody for DetectingChlamydia trachomatisInfections in Endocervical Specimens from Female Patients

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