Immunochemical Analysis of Human Papillomavirus L1 Capsid Protein in Liquid-Based Cytology Samples from Cervical Lesions

Xiao, Wei; Bian, Mei-lu; Ma, Li; Liu, Jun; Chen, Ying; Yang, Bin; Wu, Qingze

doi:10.1159/000325229

Cited by 20 publications

(16 citation statements)

References 24 publications

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“…L1 protein expression has been detected in high percentage in papillomavirus-associated pathological conditions such as inflammation and low grade lesions; it has been found to be very rarely expressed in high grade lesions and in cancers [26], [31], [32]. It has been suggested that L1 protein expression tends to be turned down with increasing severity of lesions as progression of low grade intraepithelial lesions to cancer appears to be associated with corresponding reduction of L1 protein expression [33], [34].…”

Section: Discussionmentioning

confidence: 99%

Productive Infection of Bovine Papillomavirus Type 2 in the Urothelial Cells of Naturally Occurring Urinary Bladder Tumors in Cattle and Water Buffaloes

et al. 2013

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BackgroundPapillomaviruses (PVs) are highly epitheliotropic as they usually establish productive infections within squamous epithelia of the skin, the anogenital tract and the oral cavity. In this study, early (E) and late (L) protein expression of bovine papillomavirus type 2 (BPV-2) in the urothelium of the urinary bladder is described in cows and water buffaloes suffering from naturally occurring papillomavirus-associated urothelial bladder tumors.Methods and FindingsE5 protein, the major oncoprotein of the BPV-2, was detected in all tumors. L1 DNA was amplified by PCR, cloned and sequenced and confirmed to be L1 DNA. The major capsid protein, L1, believed to be only expressed in productive papillomavirus infection was detected by Western blot analysis. Immunohistochemical investigations confirmed the presence of L1 protein both in the cytoplasm and nuclei of cells of the neoplastic urothelium. Finally, the early protein E2, required for viral DNA replication and known to be a pivotal factor for both productive and persistent infection, was detected by Western blot and immunohistochemically. Electron microscopic investigations detected electron dense particles, the shape and size of which are consistent with submicroscopic features of viral particles, in nuclei of neoplastic urothelium.ConclusionThis study shows that both active and productive infections by BPV-2 in the urothelium of the bovine and bubaline urinary bladder can occur in vivo.

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Section: Discussionmentioning

confidence: 99%

Productive Infection of Bovine Papillomavirus Type 2 in the Urothelial Cells of Naturally Occurring Urinary Bladder Tumors in Cattle and Water Buffaloes

et al. 2013

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“…Cytologic diagnosis revealed a higher expression rate in LSIL than in a typical squamous cells of undetermined significance (ASCUS) and HSIL + SCC. In 71 ASCUS/LSIL without treatment, no L1-positive cases progressed in cytology; 18.75% of L1-negative cases progressed to ASC-H/HSIL (Xiao et al, 2010). In addition, the positive rates of HPVL1 decreased gradually according to the severity of cervical neoplasia (Yu et al, 2010).…”

Section: Clinical Significance Of Hpvl1 In the Cervical Intraepithelimentioning

confidence: 98%

“…The L1 capsid protein reportedly was positive in 30% of low-grade squamous intraepithelial lesion (LSIL), 12% of high-grade squamous intraepithelial lesion (HSIL) and 0% of SCCs in liquid-based cytology (Ostor, 1993) and in 43.7% of LSILs and 33.3% HSILs in cervical smears (Melsheimer et al, 2003). In the study of Xiao et al (2010) L1 capsid protein was positive in 69.79% of cervicitis, 83.53 % of CIN I, 41.81% of CIN II, 3.13% of CIN III and 0% of SCC. Cytologic diagnosis revealed a higher expression rate in LSIL than in a typical squamous cells of undetermined significance (ASCUS) and HSIL + SCC.…”

Section: Clinical Significance Of Hpvl1 In the Cervical Intraepithelimentioning

confidence: 99%

Relationship of HPVL1 and p16 expression with different cervical lesions

Wang¹,

Shi²,

Kong³

2011

Sci. Res. Essays

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“…For example, the expression of E6 and E7 is higher in high-grade squamous intraepithelial lesions (high-grade SILs) than in low-grade SILs [14]. In contrast, expression of the L1 protein is lower in high-grade SILs [15,16]. The expression patterns of HPV-derived transcripts also differ among HPV genotypes.…”

Section: Introductionmentioning

confidence: 99%

“…Loss of cell differentiation and viral genome integration from the early stage of CIN might be associated with rapid cancer development of HPV 18-infected CINs.Conversely, L1 expression was highest in HPV 52, even in high-grade SIL (CIN2 and CIN3). Usually, L1gene expression decreases as the CIN grades progress due to the lack of cellular differentiation[15,16]. In contrast to HPV 18, the high expression of the L1 gene in HPV 52-positive specimens, even in high-grade SILs, may indicate that proliferative HPV infection accompanied with cellular differentiation may be maintained in HPV 52-positive lesions.…”

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confidence: 99%

Differential expression of human papillomavirus 16-, 18-, 52-, and 58-derived transcripts in cervical intraepithelial neoplasia

Baba

Taguchi

Kawata

et al. 2020

Preprint

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Background Human papillomavirus (HPV) infection is a primary cause of cervical cancer. Although epidemiologic study revealed that carcinogenic risk differs according to HPV genotypes, the expression patterns of HPV-derived transcripts and their dependence on HPV genotypes have not yet been fully elucidated. Methods In this study, 382 patients with abnormal cervical cytology were enrolled to assess the associations between HPV-derived transcripts and cervical intraepithelial neoplasia (CIN) grades and/or HPV genotypes. Specifically, four HPV-derived transcripts, namely, oncogenes E6 and E6* , E1^E4 , and viral capsid protein L1 in four major HPV genotypes—HPV 16, 18, 52, and 58—were investigated. Results The detection rate of E6/E6* increased with CIN progression, whereas there was no significant change in the detection rate of E1^E4 or L1 among CIN grades. In addition, we found that L1 gene expression was HPV type-dependent. Almost all HPV 52-positive specimens, approximately 50% of HPV 58-positive specimens, around 33% of HPV 16-positive specimens, and only one HPV18-positive specimen expressed L1 . Conclusions We demonstrated that HPV-derived transcripts are HPV genotype-dependent. Especially, expression patterns of L1 gene expression might reflect HPV genotype-dependent patterns of carcinogenesis.

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Immunochemical Analysis of Human Papillomavirus L1 Capsid Protein in Liquid-Based Cytology Samples from Cervical Lesions

Cited by 20 publications

References 24 publications

Productive Infection of Bovine Papillomavirus Type 2 in the Urothelial Cells of Naturally Occurring Urinary Bladder Tumors in Cattle and Water Buffaloes

Productive Infection of Bovine Papillomavirus Type 2 in the Urothelial Cells of Naturally Occurring Urinary Bladder Tumors in Cattle and Water Buffaloes

Relationship of HPVL1 and p16 expression with different cervical lesions

Differential expression of human papillomavirus 16-, 18-, 52-, and 58-derived transcripts in cervical intraepithelial neoplasia

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