Immunochemical behaviour of the proteins of the orange

Cantagalli, P; Forconi, V.; Gagnoni, G.; Pieri, J

doi:10.1002/jsfa.2740230711

Cited by 12 publications

(2 citation statements)

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“…Proteins might be specific enough to characterize the various parts of citrus fruits and might be species-specific, too. Differences in protein composition of the exo-, meso-, and endocarp of citrus fruits (18) and species-specific immunogenicity of orange and lemon juice were reported (19) earlier. A method based on immunodiffusion has been worked out by Firon et al (20) for the determination of the juice content in commercial products using an antiserum developed against the total protein content of the orange juice.…”

Section: Introductionsupporting

confidence: 58%

Distribution of Various Peptides in Citrus Fruits (Grapefruit, Lemon, and Orange)

Sass-Kiss

Sass

2002

J. Agric. Food Chem.

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Tissue- and species-specific peptides of the grapefruit have been investigated by SDS-PAGE and Western blot. Five peptides from the juice and one peptide from the peel were isolated by preparative gel electrophoresis. Polyclonal antibodies were developed against them in mice. It can be established that 82, 63, and 46 kDa peptides occurred exclusively in the samples prepared from the grapefruit and the lemon juice, whereas in the orange juice, only the 82 kDa peptide could be detected. The 31 kDa peptide is characteristic for the peel samples of grapefruit and lemon. The 210 kDa peptide did not show any specificity. A 117 kDa peptide appeared in the juice and peel of grapefruit and in the peel of lemon but not in the orange. From the data of this study, it is supposed that some of the polyclonal antibodies developed against characteristic juice and peel peptides can be used to test commercial grapefruit juice products for adulteration.

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Section: Introductionsupporting

confidence: 58%

Distribution of Various Peptides in Citrus Fruits (Grapefruit, Lemon, and Orange)

Sass-Kiss

Sass

2002

J. Agric. Food Chem.

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“…It is claimed that investigations of bioactive substances and proteins might be specific enough to characterize the various citrus fruits (Clements, 1966). Differences in protein composition of the exo-, meso-, and endocarp of citrus fruits (Clements, 1966) and species-specific immunogenicity of citrus juice have been reported (Cantagalli et al, 1972). Gorinstein et al (2001a) suggested that investigation of antioxidant compounds could reveal possible differences between cultivars of the same citrus fruit.…”

Section: Introductionmentioning

confidence: 99%

Characterization of blond and Star Ruby (red) Jaffa grapefruits using antioxidant and electrophoretic methods

Gorinstein

Drzewiecki

Park

et al. 2005

Int J of Food Sci Tech

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Antioxidant and electrophoretic methods were used to characterize the quality differences between blond and Star Ruby (red) grapefruits. Dietary fibre, minerals and trace elements, total polyphenols, anthocyanins, flavonoids, phenolic and ascorbic acids were also determined. The antioxidant potential of red grapefruit was significantly higher than that of the blond fruit (P < 0.05) and correlated well with the total polyphenols (R 2 from 0.8456 to 0.9711). In both the cultivars studied, thirty-two electrophoretic bands were detected [sodium dodecyl-polyacrylamide gel eletrophoresis (SDS-PAGE)]. The main electrophoretic bands occurred between 20 and 43 kDa in both grapefruits with few minor differences between the varieties. Our findings indicate the following (i) red grapefruit is preferable: it has a higher concentration of bioactive compounds and antioxidant potential than the blond; (ii) 1, 1-diphenyl-2-picrylhydrazyl (DPPH) test is a more sensitive method for the determination of antioxidant potential; (iii) there are some minor differences in electrophoretic patterns; (iv) antioxidant and electrophoretic methods are a good combination for characterization of differences of the same citrus fruits.

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Immunological Techniques

Hsieh

Ofori

2017

Food Authentication

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Immunochemical behaviour of the proteins of the orange

Cited by 12 publications

References 8 publications

Distribution of Various Peptides in Citrus Fruits (Grapefruit, Lemon, and Orange)

Distribution of Various Peptides in Citrus Fruits (Grapefruit, Lemon, and Orange)

Characterization of blond and Star Ruby (red) Jaffa grapefruits using antioxidant and electrophoretic methods

Immunological Techniques

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