Immunochemical determination of CK-MB isoenzyme in human serum. II. An enzymic approach.

Wicks, R W; Usategui-Gomez, M.; Miller, M. C.; Warshaw, Meredith L.

doi:10.1093/clinchem/28.1.54

Cited by 44 publications

(18 citation statements)

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“…Body and heart weights were compared between the groups (FC versus FHFD, MC versus MHFD, and FHFD versus MHFD). Lipid and glucose tests from blood and tissues were done using diagnostic enzyme assay kits (Spinreact®, Spain) such as total lipids (TL) [ 35 ], total cholesterol (TC) [ 36 ], cholesterol in high-density lipoprotein (HDL-C) [ 37 ], low-density lipoprotein (LDL-C) [ 38 ], triglycerides (TG) [ 39 ], phospholipids (PL) [ 34 , 40 ], glucose (Glc) [ 41 ], CK-MB [ 42 ], calcium [ 43 ], and proteins [ 35 ].…”

Section: Methodsmentioning

confidence: 99%

Biochemical and Ultrastructural Cardiac Changes Induced by High-Fat Diet in Female and Male Prepubertal Rabbits

Sibouakaz

Othmani-Mecif

Fernane

et al. 2018

Analytical Cellular Pathology

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Early weight gain induced by high-fat diet has been identified as a predictor for cardiac disease, one of the most serious public health problems. Our goal is to study the influence of a HFD on biochemical, oxidant stress parameters, and the cardiac ultrastructure in both male and female prepubertal models. Experiments were carried on 24 prepubertal New Zealand white rabbits, randomly assigned to male and female control (MC and FC, resp.) or HFD (MHFD and FHFD, resp.) groups (n = 6) for 3 months. Body and heart weights and some biochemical and oxidative stress parameters such as lipids, calcium, CKMB, MDA, uric acid, ascorbic acid, and AOA are evaluated in plasma and the left ventricle. Under HFD effect, plasma parameters, such as lipids (TL, PL, and LDL-C), MDA, and CK-MB, increase more significantly in male than in female groups, when AA decreases. Some cardiac parameters such as TG and UA increase, when AA and AOA decrease; these variations are more significant in FHFD. In both male and female rabbits, HFD caused changes in heart ultrastructure, junctional complexes, mitochondria size and form, and so on. Early HFD feeding induced overweight, oxidative stress, and metabolic alterations in plasma and the heart of prepubertal rabbits, whereas lipotoxicity has especially a negative impact on male plasma but affects more the female heart ultrastructure.

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Section: Methodsmentioning

confidence: 99%

Biochemical and Ultrastructural Cardiac Changes Induced by High-Fat Diet in Female and Male Prepubertal Rabbits

Sibouakaz

Othmani-Mecif

Fernane

et al. 2018

Analytical Cellular Pathology

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“…The diabetic who died suddenly during a readmission and had multiple infarcts of varying ages documented at autopsy despite negative enzymes may fit in this category. However, in MI patients the MB almost always remains elevated longer than 24 hours 10 , 11 . The three patients in this study who had an elevation of MB on one occasion had no supporting evidence for MI, and we attributed the MB elevations to laboratory error.…”

Section: Discussionmentioning

confidence: 58%

“…Serum was collected and assayed for total CK and CK‐MB once every 24 hours (more often if an abnormal level of CK‐MB was noted). In patients with myocardial necrosis, CK‐MB almost always remains elevated for longer than 24 hours, 10 , 11 but it is remotely possible a q24‐hour sampling might have missed the appearance, peak, and decline phases of CK‐MB release in a patient with perioperative MI.…”

Section: Methodsmentioning

confidence: 99%

The Incidence of Perioperative Myocardial Infarction with Transurethral Resection of the Prostate

Ashton

Lahart

Wray

1989

J American Geriatrics Society

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We performed a prospective study of 250 men undergoing transurethral resection of the prostate to determine the incidence of perioperative myocardial infarction. The prevalence of coronary artery disease in the study group was 27%. Patients had measurement of total creatine kinase and its MB isoenzyme and electrocardiography preoperatively and on the first three postoperative days. Only one myocardial infarction was diagnosed, an incidence rate of 0.4%. The overall rate of serious post-operative complications was 3.6%. No deaths occurred during the operative hospitalization. We conclude that with transurethral resection perioperative myocardial infarction is a rare event despite the high prevalence of coronary artery disease in this surgical population. Routine postoperative surveillance with electrocardiograms and creatine kinase determinations in asymptomatic patients is not warranted.

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“…Secondly, the clinician should know which method the hospital laboratory is using to separate and measure CK isoenzymes. We used a specific detection system for the MB isoenzyme that eliminates interference from other isoenzymes, atypical CKs, and adenylate cyclase 14 . Qualitative and semi‐quantitative methods such as electrophoresis and column chromatography are less specific.…”

Section: Discussionmentioning

confidence: 99%

The Frequency and Significance of ECG Changes after Transurethral Prostate Resection

Ashton

Thomas

Wray

et al. 1991

J American Geriatrics Society

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Although many clinicians routinely recommend a base-line preoperative electrocardiogram (ECG) and obtain frequent postoperative ECGs to screen for myocardial infarction or ischemia, the diagnostic utility of screening perioperative ECGs is unknown. The present analysis evaluates the sensitivity and specificity of the perioperative ECG and examines its value as a predictor of early postoperative cardiac events and outcomes during the postoperative year. ECGs obtained preoperatively and on the first 3 postoperative days in 206 men undergoing transurethral prostate resection were analyzed using the Minnesota Code. The occurrence of cardiac events during the operative stay was assessed by measurement of the cardiospecific MB creatine kinase isoenzyme on the first 3 postoperative days and review of the entire clinical course. Twenty-one percent of patients developed postoperative ECG changes, mostly involving the T wave; none had cardiac symptoms or sustained creatine kinase MB elevation. Changes were not significantly more common in men known to have coronary disease. The single patient who had a perioperative myocardial infarction confirmed by enzymes had no codable ECG changes. The specificity of any ECG change for perioperative infarction was 78%; of ST segment changes only, 95%. Only one of the patients (2%) who had postoperative ECG changes had a cardiac event in the year after surgery. Routine perioperative ECGs is of little diagnostic/predictive utility in situations in which the incidence of perioperative myocardial infarction is low.

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Immunochemical determination of CK-MB isoenzyme in human serum. II. An enzymic approach.

Cited by 44 publications

References 0 publications

Biochemical and Ultrastructural Cardiac Changes Induced by High-Fat Diet in Female and Male Prepubertal Rabbits

Biochemical and Ultrastructural Cardiac Changes Induced by High-Fat Diet in Female and Male Prepubertal Rabbits

The Incidence of Perioperative Myocardial Infarction with Transurethral Resection of the Prostate

The Frequency and Significance of ECG Changes after Transurethral Prostate Resection

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