Immunochemical Measurement of Placental Tissue Protein 4 in Serum

Shirotake, S; Inaba, Noriyuki; Ohta, Yoriko; Fukazawa, I.; Takamizawa, H; Bohn, H.

doi:10.1248/cpb.34.2191

Cited by 9 publications

(4 citation statements)

References 5 publications

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“…These immunohistochemical studies are in agreement with clinical observations that sug gest that serum PP4 levels are increased in patients with nontrophoblastic tumors, re gardless of the tumor histology [5,6], At the same time our findings contradict the data of other authors [8,11], suggesting that ectopic PP4 production is a specific characteristic of adenocarcinoma.…”

supporting

confidence: 92%

“…Lev els of PP4 correlate with progression or regres sion of disease in patients with malignant gynecological tumors [5,6,[8][9][10]. These latter findings suggest that these tumors may con tain PP4 antigen.…”

Section: Introductionmentioning

confidence: 97%

“…Placental protein 4 (PP4) is a 35,000-dalton glycoprotein first detected in 1972 [1] and subsequently isolated from human placenta in 1985 [2], This protein may function as a ble-antibody radioimmunoassay, we reported that PP4 could be detected in trace amounts in 61 % of nonpregnant women's sera; while in patients with nontrophoblastic gynecological tumors, the serum PP4 levels were significant ly higher than in pregnant women [7][8][9]. Lev els of PP4 correlate with progression or regres sion of disease in patients with malignant gynecological tumors [5,6,[8][9][10].…”

Section: Introductionmentioning

confidence: 97%

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Immunohistochemical Localization of Pregnancy–Related Placental Protein 4 in Nontumorous Tissues and in Gynecological Tumors

Göcze¹,

Józsa²,

Szabò³

et al. 1994

Tumor Biol

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The aim of the study was to demonstrate the localization of placental protein 4 (PP4) in different nontumorous and tumorous tissues originating from the female genital tract. PP4 immunoreactivity was demonstrated using the peroxidase-antiperoxidase immunohistochemical technique. Tissue samples were obtained from the cervix and body of the uterus, ovary, vulva and from gestational trophoblastic tumors. PP4-positive cells were present in nontumorous tissues with various pathologic findings, and in many but not all benign gynecological tumors. Similar numbers of PP4-positive cells were located in malignant and benign gynecological tumors; however, PP4 staining intensity was greater in the malignant lesions. PP4-positive cells were found in hydatidiform moles and in choriocarcinoma. PP4 was distributed mainly in the cytoplasm, but it was also bound to the cell membrane. We conclude from these studies that PP4 is located in a variety of benign and malignant cells of the female genital tract and that these cells may be the source of plasma PP4 found in patients with these conditions.

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supporting

confidence: 92%

Section: Introductionmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 97%

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Immunohistochemical Localization of Pregnancy–Related Placental Protein 4 in Nontumorous Tissues and in Gynecological Tumors

Göcze¹,

Józsa²,

Szabò³

et al. 1994

Tumor Biol

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“…The principle method of EIA in the present study has been already described by Shirotake et al [24]. Fifty microliter of tracer containing 2.5 ng of PP4, and 100 ~tl of sample or standard PP4 were added to each well of anti-PP4 coated microptates.…”

Section: Methodsmentioning

confidence: 99%

Enzyme immunoassay for placental protein 4 (PP4) and its possible diagnostic significance in patients with genital tract cancer

Ota

Inaba

Shirotake

et al. 1990

Arch Gynecol Obstet

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We have established an enzyme immunoassay for placental protein 4 (PP4), by using avidin-biotin binding reaction, and set its normal range below 10.9 ng/ml (mean + 2 sigma). Throughout the menstrual cycle, the serum PP4 profile was similar to that of serum progesterone. In the follicular and ovulatory phase, PP4 remained relatively low, with the mean levels of 1.5 ng/ml and 1.8 ng/ml, respectively. In the luteal phase, the mean level was 3.2 ng/ml. In normal pregnancy, serum PP4 levels were low irrespective of gestational age, with a mean level of 3.0 ng/ml. There was only one case in which the serum PP4 level over 10.9 ng/ml. Mean serum PP4 levels and the frequencies of elevated serum PP4 levels were respectively 6.3 ng/ml and 11% in patients with benign ovarian neoplasms, 4.7 ng/ml and 6% in patients with endometriosis, and 5.5 ng/ml and 18% in patients with uterine myomata. The frequency of raised PP4 levels was 48% and the mean value was 13.3 ng/ml in patients with endometrial carcinoma, and the values were 44% and 13.4 ng/ml respectively in patients with cervical carcinoma. In patients with ovarian malignancy, the respective values were 15% and 7.0 ng/ml. The results did not relate to clinical stages of disease (FIGO), while the frequencies of elevated serum PP4 in patients with uterine carcinoma was over 40% in stage I diseases. Compared with other tumor markers such as carcino-embryonic antigen (CEA), tissue polypeptide antigen (TPA) and cancer antigen 125 (CA125), PP4 seems to be more promising as a marker of endometrial carcinoma.(ABSTRACT TRUNCATED AT 250 WORDS)

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PP4 — Ein potentieller Tumormarker für gynäkologische Tumore

Hoffmann

Auerbach

Valet

et al. 1991

Gynäkologie Und Geburtshilfe 1990

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Immunochemical Measurement of Placental Tissue Protein 4 in Serum

Cited by 9 publications

References 5 publications

Immunohistochemical Localization of Pregnancy–Related Placental Protein 4 in Nontumorous Tissues and in Gynecological Tumors

Immunohistochemical Localization of Pregnancy–Related Placental Protein 4 in Nontumorous Tissues and in Gynecological Tumors

Enzyme immunoassay for placental protein 4 (PP4) and its possible diagnostic significance in patients with genital tract cancer

PP4 — Ein potentieller Tumormarker für gynäkologische Tumore

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Immunochemical Measurement of Placental Tissue Protein 4 in Serum

Cited by 9 publications

References 5 publications

Immunohistochemical Localization of Pregnancy&ndash;Related Placental Protein 4 in Nontumorous Tissues and in Gynecological Tumors

Immunohistochemical Localization of Pregnancy&ndash;Related Placental Protein 4 in Nontumorous Tissues and in Gynecological Tumors

Enzyme immunoassay for placental protein 4 (PP4) and its possible diagnostic significance in patients with genital tract cancer

PP4 — Ein potentieller Tumormarker für gynäkologische Tumore

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Product

Resources

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Immunohistochemical Localization of Pregnancy–Related Placental Protein 4 in Nontumorous Tissues and in Gynecological Tumors

Immunohistochemical Localization of Pregnancy–Related Placental Protein 4 in Nontumorous Tissues and in Gynecological Tumors