Immunochemical quantitation of antigens by single radial immunodiffusion

Solubilization from spinach thylakoids of a higher molecular weight form of ferredoxin‐NADP+ reductase

“…The precipitate was redissolved in 50 mM Tris-HCl/SO mM NaCl (pH 7.5) (4"C), dialyzed and applied on a calibrated Sephadex G-100 column (2.2 X 95 cm) equilibrated with the same buffer. The eluted fractions were tested for activity and the amount of the enzyme quantitatively determined by immunodiffusion according to [7]. The antibody used was a purified y-globulin fraction, obtained by ammonium sulfate fractionation of a rabbit antiserum raised against pure ferredoxin-NADP' reductase.…”

Purification and characterization of C4‐binding protein from human serum

“…C4bp purification was evaluated from an arbitrary A28o for ~r ~q% = 10.0. C4bp was selectively total proteins ,,,,~ 1 cm estimated by radial immunodiffusion [18] using 1% agarose and 1% anti-C4bp rabbit serum. Double immunodiffusion in 1% agarose according to [19] was used for identification of the proteins.…”

Overexpression of Frat1 in transgenic mice leads to glomerulosclerosis and nephrotic syndrome, and provides direct evidence for the involvement of Frat1 in lymphoma progression

“…Total amounts of albumin were determined using radial immunodi usion according to (Mancini et al, 1965). For routine analysis, a semiquantitative determination of protein in urine samples was obtained by re¯ectance photometric analysis of Combur 9 urine test strips with the Urotron RL 9 photometer (Boehringer Mannheim).…”