Immunochemical quantitation of UV-induced oxidative and dimeric DNA damage to human keratinocytes

Cooke, Marcus S.; Mistry, Nalini; Ladapo, Akin; Herbert, Karl E.; Lunec, Joseph

doi:10.1080/10715760000300911

Cited by 36 publications

(27 citation statements)

References 34 publications

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“…In these experiments, monochromatic lights of wavelength 320, 330 and 340 nm showed more toxic eŠects on ry 506 than the wild-type strain (unpublished observations). These results are not in agreement with previous reports (6)(7)(8). When Drosophila DNA in solution was irradiated with 20 kJ/m 2 of UVB light (302 nm peak wavelength), 8-OHdG residue levels increased 6-fold; however, there was no change in the response to irradiation with 300 kJ/m 2 of UVA (unpublished observations).…”

Section: Discussioncontrasting

confidence: 55%

“…In contrast, UVA is thought to induce mainly oxidative damage via formation of active oxygen species (5). In addition, UVA and UVB both induce oxidative damage, as revealed by an increase in the levels of 8-hydroxydeoxyguanosine (8-OHdG) residues in DNA from irradiated cells (6)(7)(8) and human epidermis (9). 8-OHdG is widely investigated as a maker of oxidative DNA damage.…”

Section: Introductionmentioning

confidence: 99%

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Evaluation of Oxidative Damage Induced by Natural Sunlight in Drosophila

Arakawa¹,

Terao²,

Hayashi³

et al. 2006

Genes and Environment

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The sunlight that reaches the surface of the earth is composed of polychromatic light with wavelengths ranging from 290 nm to 2500 nm. Ultraviolet (UV) light is a component of sunlight that is harmful to organisms. Although it is known that sunlight induces photoproducts and harmful eŠects such as major DNA damage caused by UV light in the tissues of many species including human skin, it is not clear whether sunlight induces oxidative damage in organisms. In this study, we investigated whether sunlight causes oxidative damage in exposed organisms using Drosophila. Third instar larvae were exposed to sunlight for an evaluation of viability and 8-hydroxydeoxyguanosine (8-OHdG) content. Urate-null Drosophila mutant larvae (ry 506 ), which are sensitive to active oxygen-producing agents, were more sensitive to sunlight lethality than the wild-type strain under limited conditions. This sunlight-induced toxicity in ry 506 larvae was partially prevented by pretreatment of larvae with 400 mM uric acid. The level of 8-OHdG in DNA showed no signiˆcant increase in both strains. In contrast, sunlight was signiˆcantly mutagenic for all seasons as reported previously. These results suggest that sunlight is partly responsible for oxidative damage in Drosophila and that 8-OHdG-formation plays little or no role in sunlight-induced mutation and toxicity.

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Section: Discussioncontrasting

confidence: 55%

Section: Introductionmentioning

confidence: 99%

Evaluation of Oxidative Damage Induced by Natural Sunlight in Drosophila

Arakawa¹,

Terao²,

Hayashi³

et al. 2006

Genes and Environment

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“…(49) Our study demonstrated that UVA induced a dose-dependent increase in 8-OH-dG with a fixed concentration of 8-MOP in keratinocytes. PUVA produced both singlet oxygen and superoxide anions in an in vitro system.…”

Section: Discussionmentioning

confidence: 88%

Formation of 8‐hydroxy‐2′‐deoxyguanosine in the DNA of cultured human keratinocytes by clinically used doses of narrowband and broadband ultraviolet B and psoralen plus ultraviolet A

et al. 2006

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Psoralen plus ultraviolet A (PUVA) and narrowband ultraviolet B (UVB) are widely used in skin disease phototherapy. Recently, the efficacy of UVB therapy has been greatly improved by narrowband UVB, compared to conventional broadband UVB. The objectives of the current study were to evaluate the influence of UVB-induced and PUVA-induced oxidative stress on cultured keratinocytes. We analyzed 8-hydroxy-2′ ′ ′ ′-deoxyguanosine (8-OH-dG) in human keratinocytes (HaCaT cell line) using a highperformance liquid chromatography system equipped with an electrochemical detector. Non-irradiated human keratinocytes contained a baseline of 1.48 ± ± ± ± 0.22 (mean ± ± ± ± SD) 8-OH-dG per 10 6 deoxyguanosine (dG) residues in cellular DNA, which increased linearly with higher doses of UVB. When their abilities to induce 8-OH-dG were compared to each other, based on the minimal erythemal and therapeutically used doses, by irradiating them with broadband UVB at 100 mJ/cm 2 , the amount of 8-OH-dG increased to 3.42 ± ± ± ± 0.46 residues per 10 6 dG, while a narrowband UVB treatment at 1000 mJ/cm 2 , with biological effects comparable to those elicited by 100 mJ/cm 2 broadband UVB, increased it to 2.06 ± ± ± ± 0.31 residues per 10 6 dG. PUVA treatment, with 100 ng/mL 8-methoxypsoralen and 5000 mJ/cm 2 UVA, increased the 8-OH-dG level to 4.52 ± ± ± ± 0.42 residues per 10 6 dG. When HaCaT cells treated with 2000 mJ/cm 2 narrowband UVB were cultured and the amount of 8-OH-dG was monitored in the living cells, 65.6% of the residues were repaired 24 h after treatment. Our study provides a warning that widely used narrowband UVB and PUVA induce cellular oxidative DNA damage at the therapeutically used doses, although to a lesser degree than broadband UVB with the same clinically effective dose. (Cancer Sci 2006; 97: 99 -105) E ight-hydroxy-2′-deoxyguanosine (8-OH-dG), also known as 7,8-dihydro-8-oxo-deoxyguanosine (8-oxo-dG), (1) has been proposed as a key biomarker of oxidative DNA damage relevant to carcinogenesis (1,2) and pathogenesis of autoimmune disorders. (3,4) This DNA damage is induced by the reactions of reactive oxygen species (ROS), such as hydrogen peroxide (H 2 O 2 ), superoxide anions ( ), singlet oxygen and hydroxyl radicals (·OH).Human skin is constantly exposed to environmental stresses, and is vulnerable to the effects of ROS generated by exposure to ultraviolet (UV) radiation.(5) Yamamoto et al. (6) reported that the formation of 8-OH-dG in DNA might be one of the mechanisms of daylight-induced mutagenesis. In fact, irradiation with a fluorescent sun lamp or with UVB does induce 8-OH-dG in the epidermis of hairless mice. (7,8) Parrish and Jaenicke (9) found that 313 nm UVB radiation is the most effective wavelength for the treatment of psoriasis. This finding provided the impetus for developing the Philips TL-01 fluorescent bulb, a narrowband UVB light source that produces a spectral emission between 310 and 315 nm. Narrowband UVB phototherapy has thus significantly improved the therapeutic efficacy of conventional br...

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“…The mechanism of UV radiation associated dermal damage includes, decreased collagen I and III synthesis, increased collagen degradation by TGF-β and activator protein A, infiltration of inflammatory cells predominantly by neutrophils into the dermis releasing ROS (Saha, 2012;Sorg et al, 2005;Talwar et al, 1995). Biomolecules weakly absorb UVA, but it can generate ROS, which oxidize proteins, DNA, and lipids (Cooke et al, 2000;Hattori et al, 1996;Struthers et al, 1998). Cells have developed defense systems to protect themselves from ROS, including endogenous, exogenous and enzymatic antioxidants (Dekker et al, 2005).…”

Section: Uv Rays and Skin Cancersmentioning

confidence: 99%

Botanical Antioxidants for Skin Health in the World of Cosmeceuticals

Simo

Kawal

Paliyath

et al. 2014

IJANHS

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The desire to maintain a youthful appearance in an aging population has accelerated several advancements in the cosmeceuticals market. The term cosmeceutical defines products containing bioactive substances that cannot be considered cosmetics or drugs. A variety of ingredients have been used in cosmeceuticals to improve the health and appearance of aged skin, and during the past decade, the utility of botanical natural products have gained much attention in the West. Throughout this review, the skin aging, and photoaging are discussed, mechanisms which underlie these processes are explored, and treatment options using natural plant extracts are examined.

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Immunochemical quantitation of UV-induced oxidative and dimeric DNA damage to human keratinocytes

Cited by 36 publications

References 34 publications

Evaluation of Oxidative Damage Induced by Natural Sunlight in Drosophila

Evaluation of Oxidative Damage Induced by Natural Sunlight in Drosophila

Formation of 8‐hydroxy‐2′‐deoxyguanosine in the DNA of cultured human keratinocytes by clinically used doses of narrowband and broadband ultraviolet B and psoralen plus ultraviolet A

Botanical Antioxidants for Skin Health in the World of Cosmeceuticals

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