Immunochistochemical Analysis of the Localization of Neuropeptides in the Adrenal Gland

Kondo, Hisatake

doi:10.1679/aohc.48.453

Cited by 127 publications

(52 citation statements)

References 92 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Ultrastructural profile of nucleus (NG) in a partition-like process in continuity with, but at some distance from, the glia thinly enveloping the immunopositive neuron. Bar represents 1 µm (DAB) as the immuno-histological marker is advantageous in identification of any target cells in both immuno-light and electron microscopy, as having been clearly demonstrated in many immunohistochemical studies of mammalian specimens as well as in our previous and present studies (Kondo, 1985;Stirling, 1990;Kondo & Hipkaeo, 2013). By the technical advantage, the present immunoelectron microscopy clearly disclosed that the immunostained cells were not directly exposed to the interstitial spaces, but their surfaces were enveloped by immunonegative thin processes.…”

Section: Discussionsupporting

confidence: 64%

Identification in Immuno-Electron Microscopy of Solitary Multi-Polar Peripheral Neurons of Adult Opisthorchis viverrini by Antibody Against Rat Diacylglycerol Kinaseζ

et al. 2016

Self Cite

View full text Add to dashboard Cite

SUMMARY:By utilizing the antibody for rat DGKζ a substantial number of immunopositive cells were found in the OV (Opisthorchis viverrini). The immunopositive cells appeared solitarily and they were distributed rather symmetrically to the longitudinal axis of the OV. Some of them were located in close proximity to internal organs such as uterus, ovary, testes, vitelline glands and guts. The immunostained cells extended tapering processes horizontally or obliquely to the OV longitudinal axis. In immuno-electron microscopy, the immunopositive cells were characterized by intensely immunostained mitochondria and weakly immunostained cytoplasm and immunonegative chromatin-poor nucleus. Vacuoles of various sizes without the immunoreactivity were also contained in the cells. Thin cellular processes without the immunoreactivity were found to enclose thinly the entire surfaces of the immunostained cells and processes, and they were in continuity with the interstitial partition-like processes which contained nuclei and aggregation of microfibrils at some distance from the cytoplasmic envelopes. The present finding suggests the possibility that the immunostained cells were peripheral neurons enveloped by peripheral glia and that the glia are of mesenchymal origin because of their cytoplasmic continuity to the interstitial partition-like processes. The motor or sensory nature of the neurons remains to be elucidated.

show abstract

Section: Discussionsupporting

confidence: 64%

Identification in Immuno-Electron Microscopy of Solitary Multi-Polar Peripheral Neurons of Adult Opisthorchis viverrini by Antibody Against Rat Diacylglycerol Kinaseζ

et al. 2016

Self Cite

View full text Add to dashboard Cite

show abstract

“…It is worth noting that the localization of SSTR3 in the primary cilia of endocrine cells is restricted to the two main target cells of somatostatin in the body. Among other endocrine cells, somatostain is secreted by thyroid parafollicular cells (13), adrenal chromaffin cells (15), gut endocrine cells (12), and dispersed endocrine cells in the respiratory (8) and urogenital tracts (29). However, no adjacent endocrine and exocrine cells were provided with the SSTR3-expressing primary cilia in endocrine systems other than the adenohypophysis and pancreas, though the primary cilia immunoreactive for ACIII body.…”

Section: Discussionmentioning

confidence: 99%

Restricted expression of somatostatin receptor 3 to primary cilia in the pancreatic islets and adenohypophysis of mice

2011

View full text Add to dashboard Cite

The primary cilium is now considered to function as a fundamental, not rudimentary, structure for mechanical and chemical sensing by individual cells. Primary cilia in neurons express type III adenylyl cyclase (ACIII) and GPCRs for somatostatin (somatostatin receptor 3, SSTR3), serotonin, and melanin-concentrating hormone. The present immunohistochemical and electron microscopic study revealed an abundant occurrence of SSTR3-expressing solitary cilia in insulin-and growth hormone-secreting cells of the mouse. The SSTR3 immunoreactivity was restricted to the plasma membrane of cilia in both cell types, differing from previously reported immunohistochemical localization of SSTRs to cell bodies. The primary cilia in the islet cells were longer than those in the pituitary cells and extended for a long distance in the intercellular canalicules endowed with microvilli. No other endocrine organs were provided with the SSTR3-expressing primary cilia, while the primary cilia in these organs were frequently immunolabeled with ACIII antibody. Since the somatostatin inhibition of both insulin and GH release is regulated mainly by SSTR1 and SSTR5, the primary cilia expressing SSTR3 may be involved in a signaling which differs from that via other SSTR subtypes expressing in cell bodies.

show abstract

“…Pituitary hormones and innervations, including sympathetic and sensory nerves, also regulate the adrenal cortex secretion of hormones. The nerves innervating the adrenal cortex include heterogeneous populations containing various different neuropeptides (Kondo, 1985). The sympathetic innervation is composed of cholinergic preganglionic fibers and catecholaminergic postganglionic fibers that are positive for tyrosine hydroxylase (TH) and NPY (Kondo, 1985;Holgert et al, 1998).…”

Section: In Vitro Methodsmentioning

confidence: 99%

“…Most of the extrinsic innervations in the adrenal derive from the sympathetic nervous system, including cholinergic fibers containing nitric oxide synthase (Holgert et al, 1995), thyrosine hydroxylase-and neuropeptide Y-positive postganglionic sympathetic fibers (Holgert et al, 1998;Kondo, 1985). Encephalin was exclusively found in choline-acethyl-transferase positive fibers among adrenaline chromaffin cells (Holgert et al, 1995).…”

Section: Ovarian and Adrenal Innervationsmentioning

confidence: 97%

Hormonal and Neural Mechanisms Regulating Hormone Steroids Secretion

Domı́nguez¹,

Flores²,

Cruz-Morales³

2012

Steroids - Basic Science

View full text Add to dashboard Cite

Immunochistochemical Analysis of the Localization of Neuropeptides in the Adrenal Gland

Cited by 127 publications

References 92 publications

Identification in Immuno-Electron Microscopy of Solitary Multi-Polar Peripheral Neurons of Adult Opisthorchis viverrini by Antibody Against Rat Diacylglycerol Kinaseζ

Identification in Immuno-Electron Microscopy of Solitary Multi-Polar Peripheral Neurons of Adult Opisthorchis viverrini by Antibody Against Rat Diacylglycerol Kinaseζ

Restricted expression of somatostatin receptor 3 to primary cilia in the pancreatic islets and adenohypophysis of mice

Hormonal and Neural Mechanisms Regulating Hormone Steroids Secretion

Contact Info

Product

Resources

About

Immunochistochemical Analysis of the Localization of Neuropeptides in the Adrenal Gland

Cited by 127 publications

References 92 publications

Identification in Immuno-Electron Microscopy of Solitary Multi-Polar Peripheral Neurons of Adult Opisthorchis viverrini by Antibody Against Rat Diacylglycerol Kinase&#950;

Identification in Immuno-Electron Microscopy of Solitary Multi-Polar Peripheral Neurons of Adult Opisthorchis viverrini by Antibody Against Rat Diacylglycerol Kinase&#950;

Restricted expression of somatostatin receptor 3 to primary cilia in the pancreatic islets and adenohypophysis of mice

Hormonal and Neural Mechanisms Regulating Hormone Steroids Secretion

Contact Info

Product

Resources

About

Identification in Immuno-Electron Microscopy of Solitary Multi-Polar Peripheral Neurons of Adult Opisthorchis viverrini by Antibody Against Rat Diacylglycerol Kinaseζ

Identification in Immuno-Electron Microscopy of Solitary Multi-Polar Peripheral Neurons of Adult Opisthorchis viverrini by Antibody Against Rat Diacylglycerol Kinaseζ