Immunochromatographic Assay for Simultaneous and Quantitative Detection of 3-Methyl-Quinoxaline-2-Carboxylic Acid and Quinoxaline-2-Carboxylic Acid Residues in Animal Tissues Based on Highly Luminescent Quantum Dot Beads

Xie, Yong; Zhang, Lei; Sun, Qi; Zhang, Zhihao; Le, Tao

doi:10.1007/s12161-017-1003-2

Cited by 10 publications

(4 citation statements)

References 25 publications

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“…Correlation of the results of colorimetric and fluorescence detection with HPLC values was calculated to determine the reliability of the dual-modal aptasensor. 37…”

Section: Validation Of the Dual-modal Aptasensormentioning

confidence: 99%

A label-free dual-modal aptasensor for colorimetric and fluorescent detection of sulfadiazine

Yang

Chen

et al. 2022

J. Mater. Chem. B

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“…Correlation of the results of colorimetric and fluorescence detection with HPLC values was calculated to determine the reliability of the dual-modal aptasensor. 37…”

Section: Validation Of the Dual-modal Aptasensormentioning

confidence: 99%

A label-free dual-modal aptasensor for colorimetric and fluorescent detection of sulfadiazine

Yang

Chen

et al. 2022

J. Mater. Chem. B

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“…The fluorescent label may be an inorganic or organic material, such as CdSe/ZnS core-shell quantum dots (QDs), lanthanide chelates, fluorescent proteins, fluorescent microspheres, and so on [20,21]. As a class of fluorescent semiconductor nanocrystals with unique optical properties, such as a high quantum yield, high photostability, large molar extinction coefficient, wide absorption, and narrow fluorescence emission spectrum, QDs are considered to be promising fluorescent labeling materials for use in immunoassays [22]. At present, QDs have been widely used in the biomedical and food safety fields as labeling materials for immunofluorescence probes [22][23][24].…”

Section: Introductionmentioning

confidence: 99%

“…As a class of fluorescent semiconductor nanocrystals with unique optical properties, such as a high quantum yield, high photostability, large molar extinction coefficient, wide absorption, and narrow fluorescence emission spectrum, QDs are considered to be promising fluorescent labeling materials for use in immunoassays [22]. At present, QDs have been widely used in the biomedical and food safety fields as labeling materials for immunofluorescence probes [22][23][24]. However, as yet, no FIA has been reported for DOX, and a simple and rapid QD-based FIA (QD-cFIA) is urgently needed.…”

Section: Introductionmentioning

confidence: 99%

Development of a Highly Specific Fluoroimmunoassay for the Detection of Doxycycline Residues in Water Environmental and Animal Tissue Samples

Yang

et al. 2022

Micromachines

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Doxycycline (DOX) and its metabolite residues in food and the environment pose a serious threat to human health and the ecological environment. In this work, a novel method, termed competitive fluoroimmunoassays (cFIA), based on monoclonal antibody (mAb) bio-conjugated CdSe/ZnS core–shell quantum dots (QDs), was developed for sensitive and rapid bioanalyses of DOX in natural water and commercial meats. After the optimization of the experimental conditions, 1 μg mL−1 of coating antigen and 0.5 μg mL−1 of QD-labeled mAb were used for the establishment of the cFIA. With this assay, the 50% inhibition concentration was found to be 0.35 ng mL−1 of DOX in phosphate-buffered saline samples, and the limit of detection was 0.039 ng mL−1 with minor cross-reactivity to other tetracycline members. The recoveries from natural water and commercial meats spiked with DOX concentrations of 10–600 ng mL−1 were 81.3–109.8%, and standard deviation were all below 12%. Levels measured with the QD-cFIA for thirty authentic samples were confirmed by high-performance liquid chromatography with good correlations. These results indicate that QD-cFIA is sultable for the rapid and quantitative detection of DOX residue in environmental and food samples.

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“…Quantum dots (QDs), a family of fluorescent semiconductor nanocrystals, are considered promising reporters for immunoassays due to their unique optical properties, including high quantum yield, high photostability, large molar extinction coefficient, broad absorption, and narrow fluorescent emission spectrum [ 2 , 25 ]. Recently, QDs have been used in biomolecule detections [ 27 , 28 , 29 ] and have been established as an attractive option as fluorescent probes for immunoassays [ 2 , 30 , 31 , 32 ]. However, the application of a QD-based fluorescence-linked immunosorbent assay (FLISA) for the detection of AMOZ has not yet been reported.…”

Section: Introductionmentioning

confidence: 99%

Preparation of Monoclonal Antibody and Development of Indirect Competitive Enzyme-Linked Immunosorbent Assay and Fluorescence-Linked Immunosorbent Assay for Detecting 3-Amino-5-methylmorpholino-2-oxazolidinone (AMOZ) in Edible Animal Tissue

et al. 2021

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To monitor the illegal used of furaltadone, a highly sensitive indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) and fluorescence-linked immunosorbent assay (FLISA) based on a monoclonal antibody (mAb) were developed for the detection of 3-amino-5-methylmorpholino-2-oxazolidinone (AMOZ), the major metabolite of furaltadone in animal tissues. The highly specific mAb, which was very sensitive to a nitrophenyl derivative of AMOZ (2-NP-AMOZ) with IC50 values of 0.11 and 0.09 ng/mL for ic-ELISA and FLISA, respectively, was selected for the development of immunoassays. For both the ic-ELISA and FLISA for AMOZ-spiked experiments, acceptable recovery rates of 81.1–105.3% and coefficients of variation of 4.7–9.8% were obtained. In addition, results from both ic-ELISA and FLISA methods for spiked samples’ data showed excellent correlation coefficients ranging from 0.9652 to 0.9927. Meanwhile, the proposed ic-ELISA and FLISA for thirty spiked samples were confirmed by standard LC-MS/MS with high correlation coefficients of 0.9911 and 0.9921, respectively. These results suggest that the developed ic-ELISA and FLISA are valid and cost-effective tools for high-throughput monitoring methods for AMOZ residues in animal tissues.

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Immunochromatographic Assay for Simultaneous and Quantitative Detection of 3-Methyl-Quinoxaline-2-Carboxylic Acid and Quinoxaline-2-Carboxylic Acid Residues in Animal Tissues Based on Highly Luminescent Quantum Dot Beads

Cited by 10 publications

References 25 publications

A label-free dual-modal aptasensor for colorimetric and fluorescent detection of sulfadiazine

A label-free dual-modal aptasensor for colorimetric and fluorescent detection of sulfadiazine

Development of a Highly Specific Fluoroimmunoassay for the Detection of Doxycycline Residues in Water Environmental and Animal Tissue Samples

Preparation of Monoclonal Antibody and Development of Indirect Competitive Enzyme-Linked Immunosorbent Assay and Fluorescence-Linked Immunosorbent Assay for Detecting 3-Amino-5-methylmorpholino-2-oxazolidinone (AMOZ) in Edible Animal Tissue

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