Immunochromatographic Partition of Soluble Antigens on Columns of Insoluble Diazo-Gamma-Globulins

DeCarvalho, Sergio; Lewis, Ann; Rand, H. J.; Uhrick, J. R.

doi:10.1038/204265a0

Cited by 27 publications

(4 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Rabbit Cohn fraction II (Hyland Laboratories) was polymerized with bis-diazotized benzidine by the method of DeCarvalho, Lewis, Rand, and Uhrick (1964). 15*8 ml.…”

Section: Insoluble Cross-linked Rabbit Iggmentioning

confidence: 99%

Antiglobulin factors in sera from patients with rheumatoid arthritis and normal subjects. Quantitative estimation in different immunoglobulin classes.

Torrigiani¹,

Roitt²

1967

Annals of the Rheumatic Diseases

124

View full text Add to dashboard Cite

The conventional tests for rheumatoid factor depend upon the agglutination of particles such as red cells or latex, coated with immunoglobulin-G. Since antibodies of the IgM class tend to be particularly effective in agglutination reactions, it is likely that the rheumatoid factor tests minimize any possible contribution from antiglobulin factors in other immunoglobulin classes. However, if the rheumatoid factors represent an immunological response of the patient to slightly altered autologous IgG, one would also expect to find antiglobulin factors in the IgG and IgA classes. In order to investigate this possibility we have developed a method which identifies the immunoglobulin class of the antiglobulin and also allows a quantitative assessment to be made. The method depends upon the absorption of antiglobulins on to insoluble cross-linked rabbit y-globulin, their subsequent elution from this complex, and estimation by the immunoplate technique using specific anti-immunoglobulin antisera incorporated into the gel. A preliminary account of these studies has been revorted (Torrigiani and Roitt, 1965a).Material and Methods Sera 27 sera from patients with rheumatoid arthritis all containing rheumatoid factors reacting with rabbit IgG in the sheep cell agglutination test (SCAT) were used.As controls, 21 sera from normal blood donors and four cord bloods were tested. All sera were stored at -20°C. and spun at 4,000 r.p.m. before use.

show abstract

“…Rabbit Cohn fraction II (Hyland Laboratories) was polymerized with bis-diazotized benzidine by the method of DeCarvalho, Lewis, Rand, and Uhrick (1964). 15*8 ml.…”

Section: Insoluble Cross-linked Rabbit Iggmentioning

confidence: 99%

Antiglobulin factors in sera from patients with rheumatoid arthritis and normal subjects. Quantitative estimation in different immunoglobulin classes.

Torrigiani¹,

Roitt²

1967

Annals of the Rheumatic Diseases

124

View full text Add to dashboard Cite

show abstract

“…In order to increase the immunogeneity of these antigens, we have removed the major serum proteins and AFP from specially selected samples of human amniotic fluid by means of antibody affinity chromatography. This subtractive, or negative, approach to the search for new antigens (De Carvalho, Lewis, Rand & Uhrick, 1964;Anderson et al, 1974) has been referred to by Sutcliffe (1976) as "negative antibody affinity chromatography".…”

Section: Introductionmentioning

confidence: 99%

Fetal- and uterine-specific antigens in human amniotic fluid

Sutcliffe¹,

Brock²,

Nicholson³

et al. 1978

Reproduction

View full text Add to dashboard Cite

Summary. Removal of the major maternal serum proteins from second trimester amniotic fluid by antibody affinity chromatography revealed various soluble tissue antigens, of which two were fetal-specific skin proteins and another, of \g=a\2-mobility, was specific to the uterus, and was therefore designated alpha-uterine protein (AUP). These proteins could not be detected in maternal serum by antibody\p=n-\antigen crossed electrophoresis. The concentration of AUP in amniotic fluid reached a maximum between 10 and 20 weeks of gestation, suggesting that there is an influx of uterine protein into the amniotic fluid at this stage of pregnancy.

show abstract

“…It is a pool of ser um from horses hyperimmunized with TSA [14,16], This antigen mixture was obtained by immunochromatographic separation [18,19] of fluorocarbon-extracted cancer tissue pro teins [13]. The serum utilized in the tests described in this paper was obtained from one single horse (HTU2) [19].This batch (HTU., No.34 plasma) has an immunodiffusion precipitin titer of 2 'against TSA. An ideal batch [14] has shown no titer against similarly extracted antigens from normal tissues(normal tissueantigens[NTA]).…”

Section: Methodsmentioning

confidence: 99%

Detection of Neoantigens in the Serum of Patients with Active Neoplastic Diseases by the Absorption-Immunodiffusion Method

DeCarvalho¹

1973

Oncology

Self Cite

View full text Add to dashboard Cite

Horse precipitating antibodies produced against antigens isolated from a large number of human tumors have revealed identical tumor associated antigens in the serum of many patients with active malignant disease but not in the serum of patients afflicted with a variety of disorders including infectious, inflammatory and autoimmune disease, or in the serum of healthy individuals, in persons bearing benign tumors or in the serum of cancer patients in clinical remission. In the first 791 coded serum samples studied by an absorption-immunodiffusion method, there was 97–34% consistency and accuracy, and a conditional probability for screening correctly of 86%. Intricate Ouchterlony patterns indicated complex antigenic composition and a relationship between different neoplasms. Although a common antigen could not be excluded, the presence of group specific and single tumor antigens was observed. Quantitation of antigens in the serum with view to a measure of tumor specific tolerance was attempted but no information was obtained to the moment to correlate the amount of tumor tissue with the amount of circulating tumor antigen. The value of this test in presymptomatic detection was not established at this time. It is recognized that a great deal of work is required to confirm the universal diagnostic validity as well as the clinical sensitivity of the method proposed for detecting tumor associated antigens in circulation. False positive results in sera of pregnant women are discussed in the light of recent findings on the relationship of fetal and neoantigens.

show abstract

Immunochromatographic Partition of Soluble Antigens on Columns of Insoluble Diazo-Gamma-Globulins

Cited by 27 publications

References 13 publications

Antiglobulin factors in sera from patients with rheumatoid arthritis and normal subjects. Quantitative estimation in different immunoglobulin classes.

Antiglobulin factors in sera from patients with rheumatoid arthritis and normal subjects. Quantitative estimation in different immunoglobulin classes.

Fetal- and uterine-specific antigens in human amniotic fluid

Detection of Neoantigens in the Serum of Patients with Active Neoplastic Diseases by the Absorption-Immunodiffusion Method

Contact Info

Product

Resources

About