Immunochromatographic test strip for the rapid detection of tricaine in fish samples

Lei, Xianlu; Xu, Xinxin; Liu, Liqiang; Kuang, Hua; Xu, Liguang; Hao, Changlong

doi:10.1080/09540105.2020.1752155

Cited by 23 publications

(15 citation statements)

References 28 publications

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“…Blue AuNPs were 83.74 nm (Figure 1F), while red AuNPs were 23.24 nm (Figure 1E). The red AuNPs were similar to a previous report in morphology and size [24], while our blue AuNPs had a unique size compared with the 44.3 nm blue AuNPs reported by Wu et al [17] and the 36.0 nm blue AuNPs reported by Peng et al [21], which might be caused by different addition amounts of Au seeds and hydroquinone solution. Our results indicated that the two-colour AuNPs had a uniform particle size distribution and a significant difference in optical absorption wavelength, making them suitable as coloured probes to develop a simultaneous detection method.…”

Section: Synthesis and Characterisation Of Two-colour Aunpssupporting

confidence: 89%

A Rapid Tricolour Immunochromatographic Assay for Simultaneous Detection of Tricaine and Malachite Green

Xü

Liu

et al. 2022

Biosensors

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In this research, we designed a rapid tricolour immunochromatographic test strip with double test lines (TS-DTL) and two-colour AuNP probes, which realised the simultaneous detection of tricaine mesylate (TMS) and malachite green (MG). Through a distinct tricolour system (red T1 line, blue T2 line and purple C line), a visual identification of TMS (0.2 μg/mL) and MG (0.5 μg/mL) was quickly achieved on site, which improved the accuracy of naked eye observations. The LODs of TMS in aquaculture water, fish and shrimp were 11.0, 29.6 and 61.4 ng/mL, respectively. MG LODs were 47.0 ng/mL (aquaculture water), 82.8 ng/mL (fish) and 152.4 ng/mL (shrimp). The LOD of MG was close to the similar TS methods. However, visual detection of TMS could meet the requirements of the residue limit (1 μg/mL) of TMS in the USA, and the quantitative detection of TMS was over 16 times lower than the USA standard. The developed platform was rapid (~20 min, HPLC~3 h) and accurate, which was verified using a traditional HPLC method. The recovery rates ranged from 82.2% to 108.6% in three types of real samples, indicating a potential application in on-site fast screening or multiple detection for TMS and MG residues in aquatic products.

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Section: Synthesis and Characterisation Of Two-colour Aunpssupporting

confidence: 89%

A Rapid Tricolour Immunochromatographic Assay for Simultaneous Detection of Tricaine and Malachite Green

Xü

Liu

et al. 2022

Biosensors

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“…Ofloxacin-Ovalbumin. The coating antigen ofloxacin (OFL)-ovalbumin (OVA) was obtained by the mixed-anhydride method (Lei et al, 2020b). First, 7.3 mg of OFL was dissolved in 0.7 mL of anhydrous DMF and mixed with 5.7 μL of tributylamine for 15 min in an ice bath, and then 3.2 μL of isobutyl-chloroformate was added into the solution.…”

Section: Synthesis and Characterization Of Hapten And Antigenmentioning

confidence: 99%

Immunochromatographic assays for ultrasensitive and high specific determination of enrofloxacin in milk, eggs, honey, and chicken meat

Lei

Liu

et al. 2022

Journal of Dairy Science

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Enrofloxacin, a veterinary antibiotic that persists in food, poses a risk to human health. Here, a monoclonal antibody against enrofloxacin, 1H12, was prepared based on the hapten ENR-1, and showed excellent sensitivity with a 50% inhibitory concentration (IC 50 ) of 0.03 ng/mL. Using this antibody, 2 lateralflow immunochromatographic assays were developed for determination of enrofloxacin in egg, milk, honey, and chicken samples. The detection ranges (IC 20 -IC 80 ) were 0.16-0.82 ng/g, 0.24-1.8 ng/g, 0.25-3.6 ng/g, and 0.61-3.9 ng/g by colloidal gold-immunochromatographic sensor (CG-ICS) analysis, and 0.022-0.42 ng/g, 0.054-0.42 ng/g, 0.069-1.4 ng/g, and 0.19-2.2 ng/g by Eu-fluorescence-immunochromatographic sensor (EF-ICS) analysis. The intraassay and interassay recovery rates were 88.9 to 108.5% with coefficients of variation of 1.3 to 7.0% by CG-ICS analysis, and 88.6 to 113.6% with coefficients of variation of 1.3 to 8.1% by EF-ICS analysis. Thus, our newly developed ICS are sensitive and reliable, providing an option for rapid quantitative detection of enrofloxacin in food samples.

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“…The colloidal gold-labeled mAbs were prepared. 25,26 First, we adjusted the pH of the colloidal gold solution (10 mL) to pH 8.2 with 0.1 M K 2 CO 3 . Following the addition of 80 mL of 1 mg mL À1 mAbs, we allowed the mixture to react for 50 min while gently stirring.…”

Section: Preparation Of Colloidal Gold-labeled Mab Conjugatesmentioning

confidence: 99%

A gold nanoparticle based colorimetric sensor for the rapid detection of Yersinia enterocolitica serotype O:8 in food samples

Zeng

Ding

et al. 2022

J. Mater. Chem. B

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Immunochromatographic test strip for the rapid detection of tricaine in fish samples

Cited by 23 publications

References 28 publications

A Rapid Tricolour Immunochromatographic Assay for Simultaneous Detection of Tricaine and Malachite Green

A Rapid Tricolour Immunochromatographic Assay for Simultaneous Detection of Tricaine and Malachite Green

Immunochromatographic assays for ultrasensitive and high specific determination of enrofloxacin in milk, eggs, honey, and chicken meat

A gold nanoparticle based colorimetric sensor for the rapid detection of Yersinia enterocolitica serotype O:8 in food samples

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