Immunochromatography for the rapid determination of cadmium concentrations in wheat grain and eggplant

Abe, Kazuyuki; Nakamura, Katsuo; Arao, Tomohito; Sakurai, Yasuhisa; Nakano, Ayumi; Suginuma, Chieko; Tawarada, Kei; Sasaki, Kazuhiro

doi:10.1002/jsfa.4321

Cited by 29 publications

(19 citation statements)

References 9 publications

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“…Because both Pb and Cu are heavy metals, which the national effluent standards are established in Japan, the cross‐reactivity with the corresponding heavy metals might be acceptable in terms of practicality. Alternatively, a pretreatment of the samples with an ion exchange column that traps Cd selectively might be effective . Overall, the proposed Cd biosensor had room for improvement in terms of its selectivity.…”

Section: Resultsmentioning

confidence: 99%

“…Alternatively, a pretreatment of the samples with an ion exchange column that traps Cd selectively might be effective. 28 Overall, the proposed Cd biosensor had room for improvement in terms of its selectivity. Nevertheless, its low cost and proposed simple and rapid operation should make the proposed biosensor suitable for cost-effective preliminary screening of environmental and food samples suspected of Cd contaminations, before analysis using highly sensitive and selective but high cost, time-consuming, and complicated conventional analytical techniques.…”

Section: Performance Of the Proposed Biosensor Based On Atpcs1displaymentioning

confidence: 95%

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Development of surface‐engineered yeast cells displaying phytochelatin synthase and their application to cadmium biosensors by the combined use of pyrene‐excimer fluorescence

Matsuura

Yamamoto

Muraoka

et al. 2013

Biotechnology Progress

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The development of simple, portable, inexpensive, and rapid analytical methods for detecting and monitoring toxic heavy metals are important for the safety and security of humans and their environment. Herein, we describe the application of phytochelatin (PC) synthase, which plays a critical role in heavy metal responses in higher plants and green algae, in a novel fluorescent sensing platform for cadmium (Cd). We first created surface-engineered yeast cells on which the PC synthase from Arabidopsis (AtPCS1) was displayed with retention of enzymatic activity. The general concept for the sensor is based on the Cd level-dependent synthesis of PC2 from glutathiones by AtPCS1-displaying yeast cells, followed by simple discriminative detection of PC2 via sensing of excimer fluorescence of thiol-labeling pyrene probes. The intensity of excimer fluorescence increased in the presence of Cd up to 1.0 μM in an approximately dose-dependent manner. This novel biosensor achieved a detection limit of as low as 0.2 μM (22.5 μg/L) for Cd. Although its use may be limited by the fact that Cu and Pb can induce cross-reaction, the proposed simple biosensor holds promise as a method useful for cost-effective screening of Cd contamination in environmental and food samples. The AtPCS1-displaying yeast cells also might be attractive tools for dissection of the catalytic mechanisms of PCS.

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Section: Resultsmentioning

confidence: 99%

Section: Performance Of the Proposed Biosensor Based On Atpcs1displaymentioning

confidence: 95%

Development of surface‐engineered yeast cells displaying phytochelatin synthase and their application to cadmium biosensors by the combined use of pyrene‐excimer fluorescence

Matsuura

Yamamoto

Muraoka

et al. 2013

Biotechnology Progress

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“…Some examples of applications involving small and environmentally important toxic substances are: -cadmium [206] -PAHs, PCBs [207], dioxins [208] -organophosphorus pesticides [209,210] -pyrethroid pesticides in water [211] -mycotoxins [212] and bacterial toxins [213] -the wood preservative pentachlorphenol (PCP) in soil [214] -explosives [215] -chemical warfare agents [216] …”

Section: Markers For Environmental Chemical Exposurementioning

confidence: 99%

Immunodiagnostics and Immunosensor Design

Gubala¹,

Klein²,

Templeton³

et al. 2016

IUPAC Standards Online

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This work compiles information on the principles of diagnostic immunochemical methods and the recent advances in this field. It presents an overview of modern techniques for the production of diagnostic antibodies, their modification with the aim of improving their diagnostic potency, the different types of immunochemical detection systems, and the increasing diagnostic applications for human health that include specific disease markers, individualized diagnosis of cancer subtypes, therapeutic and addictive drugs, food residues, and environmental contaminants. A special focus lies in novel developments of immunosensor techniques, promising approaches to miniaturized detection units and the associated microfluidic systems. The trends towards high-throughput systems, multiplexed analysis, and miniaturization of the diagnostic tools are discussed. It is also made evident that progress in the last few years has largely relied on novel chemical approaches.

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“…In contrast, immunoaffinity chromatography is a separation method that takes the advantage of the specific and reversible interaction between antibody and antigen, and has been considered as one of the most powerful techniques for isolation and purification of individual or classes of compounds from liquid matrices (Senyuva & Gilbert, 2010). Owing to the advantages of high specificity, simple operation and therefore low cost per sample, immunoaffinity chromatography has been widely used in sample pretreatment for residue analysis of mycotoxins (Klinglmayr, Nöbauer, Razzazi-Fazeli, & Cichna-Markl, 2010;Tavčar-Kalcher, Vrtač, Pestevšek, & Vengušt, 2007;), veterinary drugs (Wang & Shen, 2007;Zhang et al, 2011), pesticides (Chen, Wang, Wang, & Tang, 2009;Wang et al, 2011) and heavy metals (Abe et al, 2011). Our previous work has described an immunoaffinity extraction of MA from food and feed samples using a polyclonal antibody (pAb) against MA (Wang, Xu, Zhang, Wang, & Dong, 2011a).…”

Section: Development and Characterisation Of An Immunoaffinity Columnmentioning

confidence: 99%

“…Optimisation of loading and eluting conditions is necessary to IAC cleanup because these conditions have a strong influence on the association/dissociation of analyteÁ antibody complex and the activity of antibody (Abe et al, 2011). In this study, three different loading solutions (2%, 5% and 10% of methanol) were evaluated.…”

Section: Optimisation Of Extraction Conditions For Iacmentioning

confidence: 99%

Development and characterisation of an immunoaffinity column for the selective extraction of methandrostenolone from food and feed samples

Wang

Zhang

et al. 2013

Food and Agricultural Immunology

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2013)Development and characterisation of an immunoaffinity column for the selective extraction of methandrostenolone from food and feed samples, Food and Agricultural Immunology, 24:2, 231-240, Methandrostenolone (MA) is used as veterinary medicine for promoting foodproducing animal growth. Accumulation of MA in a human body would cause multiple side effects. In this study, an anti-MA monoclonal antibody (mAb)-based immunoaffinity column (IAC) was prepared and its application to the selective extraction of MA from actual samples was evaluated. The employed antibody exhibited good sensitivity and specificity towards MA with an IC 50 value of 7.89 ng/mL and less than 1% cross-reactivity towards structurally related compounds. By coupling CNBr-activated Sepharose with the antibody, an IAC was prepared. Two percent methanol and 70% methanol were selected, respectively, as loading and eluting solution by optimisation. The maximum binding capacity of the column for MA reached 4760 ng/mL gel. The average recovery of 50, 250 and 500 ng MA standards from IACs was 88.6% with a relative standard deviation (RSD) among columns of 8.5%. After three times usage, the binding capacity and recovery still remained 93.4% and 81.1%, respectively. To further verify the effect of matrices on the extraction efficiency, the IACs were challenged with MA-fortified animal tissue and feed samples and the recovery were found to be in the range of 78.7%Á95.1%.

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Immunochromatography for the rapid determination of cadmium concentrations in wheat grain and eggplant

Abstract: Approximate Cd concentrations in wheat grain and fresh eggplant can be monitored easily and quickly by this method at locations where facilities for acid digestion and precision analysis are not available.

Cited by 29 publications

References 9 publications

Development of surface‐engineered yeast cells displaying phytochelatin synthase and their application to cadmium biosensors by the combined use of pyrene‐excimer fluorescence

Development of surface‐engineered yeast cells displaying phytochelatin synthase and their application to cadmium biosensors by the combined use of pyrene‐excimer fluorescence

Immunodiagnostics and Immunosensor Design

Development and characterisation of an immunoaffinity column for the selective extraction of methandrostenolone from food and feed samples

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