Immunocontraceptive Effects in Male Rats Vaccinated with Gonadotropin-Releasing Hormone-I and -II Protein Complex

Kim, Yong Hyun; Park, Byoung Joo; Ahn, Hee-Seop; Han, Sang-Hoon; Go, Hyeon‐Jeong; Bok, Lee Joong; Yong, Park Seung; Chang-Seon, Song; Lee, Sang‐Won; Choi, In Soo

doi:10.4014/jmb.1901.01067

Cited by 7 publications

(8 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Most studies reporting the development of immunocontraceptive vaccines to produce anti‐GnRH antibodies in male livestock, laboratory animals, and pets have used GnRH‐I as an antigen (Cook et al., 2000; Dunshea et al., 2001). In a previous study, we confirmed infertility in male rats administered a vaccine composed of GnRH‐I and GnRH‐II (Kim et al., 2019). In addition, we also found enhanced immunogenicity of the GnRH vaccine conjugated with STF‐2.…”

Section: Discussionsupporting

confidence: 79%

“…Serum samples collected from each mouse in the experimental groups were used to detect antibodies specific to GnRH‐I. Anti‐GnRH‐I antibody titers were determined using an enzyme‐linked immunosorbent assay (ELISA), as previously described (Kim et al., 2019; Song et al., 2012). Testosterone concentration was determined using a Mouse Testosterone T ELISA Kit (CUSABIO, China).…”

Section: Methodsmentioning

confidence: 99%

“…However, little is known about the physiological role of GnRH‐II. Several studies have speculated that GnRH‐II is related to spermatogenesis in male animals (Khan et al., 2007; Kim et al., 2019).…”

Section: Introductionmentioning

confidence: 99%

“…Flagellin, a known ligand for Toll‐like receptor 5, significantly increases the immunogenicity of several antigens (Huleatt et al., 2008). In our previous study, we developed an immunocontraceptive vaccine containing six copies of both GnRH‐I and GnRH‐II conjugated to Salmonella typhimurium flagellin (STF‐2), which efficiently inhibited reproductive system development and spermatogenesis in male rats (Kim et al., 2019). However, we did not evaluate immunocontraception in either male or female animals.…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Induction of immunocontraceptive effects in both male and female mice immunized with GnRH vaccine

Ahn

Park

et al. 2021

Veterinary Medicine & Sci

View full text Add to dashboard Cite

Background Gonadotropin‐releasing hormone (GnRH) plays a pivotal role in regulating the reproductive endocrine system. Objective An immunocontraception vaccine aimed at inhibiting the functions of GnRH is tested as a potential tool for controlling animal populations. Methods We developed a recombinant immunocontraceptive vaccine composed of GnRH‐I and GnRH‐II (GnRH I+II), which was conjugated with Salmonella typhimurium flagellin. Forty‐eight BALB/c mice aged 4 weeks were divided into four groups (each group had n = 12): non‐vaccinated male (NVM), non‐vaccinated female (NVF), vaccinated male (VM), and vaccinated female (VF). Mice in the vaccinated groups were vaccinated twice by intramuscular injection at 0 and 2 weeks with 300 μg of the recombinant GnRH protein complex per mouse. Mice in the non‐vaccinated groups were injected with saline and served as the unimmunized controls. Twenty‐four pairs of male and female mice were mated for 10–12 weeks after initial immunization in four groups: 6 NVF × 6 NVM, 6 VF × 6 NVM, 6 NVF × 6 VM, and 6 VF × 6 VM. Results: An increase (p < 0.001) in antibody titers in VM and VF mice was observed. The testosterone levels and the number of spermatocytes were lower (p < 0.001) in VM mice than those in the control mice. The progesterone levels and the number of corpora lutea were lower (p < 0.001) than those in the control mice. Mating results in both VM and VF mice confirmed a 60% reduction in pregnancy rates and offspring numbers. Conclusions The recombinant GnRH vaccine can be used for birth control in both male and female animals.

show abstract

Section: Discussionsupporting

confidence: 79%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Induction of immunocontraceptive effects in both male and female mice immunized with GnRH vaccine

Ahn

Park

et al. 2021

Veterinary Medicine & Sci

View full text Add to dashboard Cite

show abstract

“…The main targets for immunocontraception have been GnRH, zona pellucida (ZP), and more recently GnRH receptors. GnRH hormone is synthesized by the hypothalamus and regulates the secretion of luteinizing hormone (LH) and follicle-stimulating hormone (FSH) which are hormones essential for reproduction [ 11 ].…”

Section: Introductionmentioning

confidence: 99%

Immunocontraceptive potential of a GnRH receptor-based fusion recombinant protein

Sandam

Prakash

Thimmareddy³

2021

Journal of Genetic Engineering and Biotechnology

View full text Add to dashboard Cite

Background The management of stray dog population has been of utmost importance due to their overpopulation, increase in dog bites incidence, and rabies. Contraceptive vaccines, a non-surgical alternative to spaying and neutering are viewed as a valuable option for the management of dog population. In this study, the contraceptive potential of a recombinant fusion protein containing the three genes GnRH, GnRH receptor, and ZP3 was explored. Results The gene fragment encoding GnRH, GnRHR, and ZP3 along with the antigenic epitopes of canine distemper virus and tetanus toxoid was assembled, synthesized, and cloned into pET28a expression vector. The resulting construct GVAC08 was successfully transformed into BL21DE3 strain of E. coli and confirmed by colony PCR. The recombinant GVAC08 protein was expressed and purified using Ni-NTA and was confirmed to be a 50-KDa protein by SDS PAGE and Western blot. Mice were immunized with the GVAC08 protein using Freund’s complete adjuvant followed by a booster using Freund’s incomplete adjuvant. This induced a high antibody titer against GnRH, GnRH receptor, and ZP3 which was determined by ELISA. Conclusion Mating studies showed that the GVAC08 recombinant protein was able to reduce the litter size in immunized mice showing improved efficacy. However, the vaccine candidate with further improvements will be a viable contraceptive vaccine.

show abstract

Involvement of the SCO3366 efflux pump from S. coelicolor in rifampicin resistance and its regulation by a TetR regulator

Nag

Mehra

2022

Appl Microbiol Biotechnol

View full text Add to dashboard Cite

Immunocontraceptive Effects in Male Rats Vaccinated with Gonadotropin-Releasing Hormone-I and -II Protein Complex

Cited by 7 publications

References 27 publications

Induction of immunocontraceptive effects in both male and female mice immunized with GnRH vaccine

Induction of immunocontraceptive effects in both male and female mice immunized with GnRH vaccine

Immunocontraceptive potential of a GnRH receptor-based fusion recombinant protein

Involvement of the SCO3366 efflux pump from S. coelicolor in rifampicin resistance and its regulation by a TetR regulator

Contact Info

Product

Resources

About