Immunocytochemical and ultrastructural characterization of prolactin, growth hormone, and somatolactin cells from the Mediterranean yellowtail (Seriola dumerilii, Risso 1810)

García‐Ayala, Alfonsa; García-Hernández, M.P.; Quesada, J.; Agulleiro, B.

doi:10.1002/(sici)1097-0185(199703)247:3<395::aid-ar11>3.0.co;2-k

Cited by 18 publications

(8 citation statements)

References 34 publications

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“…The columnar cells in the dorsal regions of the PPD along the neurohypophyseal boundaries resemble the STH cells described by immunocytochemistry, electron-microscopy and in situ hybridization in other teleosts (Nagahama et al, 1981;Ueda et al, 1985;Quesada et al, 1988;Toubeau et al, 1991;Nishioka et al, 1993;Parhar and Iwata, 1994;Garcia-Hernandez et al, 1996;Garcia-Ayala et al, 1997). Two forms of somatotropic hormones and two distinct STH cell populations differing in shape, immunostaining intensity and organization have been reported (Huang and Specker, 1994;Garcia-Hernandez et al, 1996).…”

Section: Discussionsupporting

confidence: 62%

“…The cross-reactivity of PRL antiserum with GH and SL cells is probably due to the molecular similarities between PRL/GH/SL genes (Ono and Kawauchi, 1994). PRL cells have been characterized on the basis of immunocytochemistry (Cambre et al, 1986;Quesada et al, 1988;Huang and Specker, 1994;Garcia-Hernandez et al, 1996), electron-microscopic immunocytochemistry (Specker et al, 1993;Garcia-Ayala et al, 1997) and recently PRL mRNA localization by in situ hybridization (Nishioka et al, 1993). As in most other fish, PRL cells are the principle component of the RPD.…”

Section: Discussionmentioning

confidence: 99%

“…In TM, it could also be a stress response while defending territory since some types of stress can activate melanotropic cells (Sumpter et al, 1985). (Rand-Weaver et al, 1991;Kaneko et al, 1993;Parhar and Iwata, 1994;Garcia-Hernandez et al, 1996;Garcia-Ayala et al, 1997) are found mainly in close proximity to the interdigitations of the neurohypophysis of the pars intermedia, and correspond to the periodic-acid-Schiff (PAS)-positive cells of other teleosts (Bern et al, 1974;Benjamin, 1979;Quesada et al, 1988). As in the Seriola dumerilii, somatolactin cells with predominantly round secretory granules and large local dilatations of endoplasmic reticulum cisternae seem to be undergoing an intense process of hormone synthesis and storage, whereas cells with very irregular granules, which seemed to result from the fusion of other secretory granule populations, might represent an active stage of granular release (GarciaAyala et al, 1997).…”

Section: Discussionmentioning

confidence: 99%

“…Dark somatolactin cells showing ultrastructural features of involutive cells have been reported in the Seriola dumerilli (Garcia-Ayala et al, 1997). The large number of cell deaths (cells with intensely electron-dense cytoplasm) and relatively inactive (or hyperactive) cells in SM may be a stress-induced response to the unnatural environment in the cages.…”

Section: Somatolactin Cells Somatolactin Cells Identified By Ultrastmentioning

confidence: 93%

“…The effects of sex steroids on behavioral or gonadal sex change could operate via the hypothalamo-hypophyseal axis. Using techniques which include histochemistry (Nagahama, 1973;Bern et al, 1974), immunocytochemistry (Munro, 1985;Quesada et al, 1988;Toubeau et al, 1991;Garcia-Hernandez et al, 1996) and ultrastructure (Bern et al, 1974;Batten, 1986;Quesada et al, 1988;Garcia-Ayala et al, 1997) the different cell types in the pituitary of teleosts have been shown to segregate between three zones of the adenohypophysis. The prolactin cells and the corticotropic cells are located in the rostral pars distalis.…”

Section: Introductionmentioning

confidence: 99%

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Immunocytochemical and Ultrastructural Identification of Pituitary Cell Types in the Protogynous Thalassoma duperrey during Adult Sexual Ontogeny

et al. 1998

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Protogynous wrasses (Thalassoma duperrey): females (F), primary males (PM) along with a few terminal-phase males (TM) and sex-changed males (SM), were used to characterize the topographical organization of the pituitary. In general, immunocytochemical and ultrastructural features of the adenohypophyseal cell types of the saddleback wrasse pituitary resemble those of other teleosts. In the rostral pars distalis (RPD), corticotropic cells were found bordering the neurohypophysis (NH) and surrounding the centroventrally located prolactin cells. Thyrotropic cells formed a small group in the anteriodorsal part of the rostral and proximal pars distalis (PPD). The somatotropic cells were distributed in large clusters, mostly organized in cell cords around the interdigitations of the NH of the dorsal PPD. Cells containing gonadotropin Ib subunit were localized in the dorsal parts of the PPD, in close association with somatotropic cells and gonadotropin IIb subunit containing cells were seen in the centroventral parts of the PPD and along the periphery of the pars intermedia (PI). The pars intermedia was composed of melanotropic cells and somatolactin cells that lined the neurohypohysis.Distinct ultrastructural differences in corticotropic and somatotropic cells were not observed between the four groups. In all groups, prolactin cells in the ventral-most RPD could be immature cells or actively secreting prolactin. Gonadotropic II cells of PM and F had relatively higher incidence of "nuclear budding" and cell organelles compared to TM and SM. Besides gonadotropic, the active melanotropic and somatolactin cells might be associated with some aspect(s) of reproduction.

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Section: Discussionsupporting

confidence: 62%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Somatolactin Cells Somatolactin Cells Identified By Ultrastmentioning

confidence: 93%

Section: Introductionmentioning

confidence: 99%

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Immunocytochemical and Ultrastructural Identification of Pituitary Cell Types in the Protogynous Thalassoma duperrey during Adult Sexual Ontogeny

et al. 1998

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Immunocytochemical and ultrastructural characterization of melanotropin and adrenocorticotropin cells from the mediterranean yellowtail (Seriola dumerilii, Risso 1810)

et al. 1997

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Gonadotropic and thyrotropic cells from the Mediterranean Yellowtail (Seriola dumerilii; Risso, 1810): Immunocytochemical and ultrastructural characterization

et al. 1998

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Background: Gonadotropins GTH I and GTH II from the pituitary of Mediterranean (M.) yellowtail (Seriola dumerilii) were isolated and characterized, and antisera to the whole GTH II molecule (anti-My ␣,␤GTH II) and to its ␤-subunit (anti-My ␤GTH II) were obtained. At the light microscopic level, anti-My ␣,␤GTH II reacted with My ␤GTH II-immunoreactive cells (GTH II cells), thyroid-stimulating hormone (TSH) cells, and a third cell population, which could have been GTH I cells. The aim of this study was the ultrastructural characterization of GTH and TSH cells in M. yellowtail using the immunogold method in order to provide a basis for future research into reproduction of this species.Methods: Pituitaries from mature male and female specimens reared in captivity were disected out and processed for electron microscopy. The immunogold method was carried out by using anti-My ␣,␤GTH II, anti-My ␣,␤GTH II preabsorbed with the ␣ subunit of the M. yellowtail GTH (My ␣GTH-subunit), anti-My ␤GTH II, anti-human (h) ␣,␤TSH, and anti-h ␤TSH sera to reveal gonadotropic and thyrotropic cells.Results: M. yellowtail gonadotropic cells were very heterogeneous with regard to their size, shape, and ultrastructural features. Cells were found with numerous, round, variably electron-dense, secretory granules and globules; others were found with their cytoplasm occupied mostly by dilated cisternae of rough endoplasmic reticulum (RER) and scarce secretory granules; and other intermediate cell forms were found that showed varying proportions of secretory granules and dilated RER. The secretory granules and globules were immunogold labeled with anti-My ␣,␤GTH II, and the reaction was weaker in the latter. A similar immunogold-labeling pattern was found with anti-My ␤GTH II and with anti-My ␣,␤GTH II preabsorbed with the My ␣GTH-subunit, although some cells that showed the same ultrastructural features described above were not immunogold labeled and could have been GTH I cells. Thyrotropic cells had small, round, secretory granules of medium or high electron density that were immunogold labeled with anti-My ␣,␤GTH II, anti-h ␣,␤TSH, and anti-h ␤TSH sera, but not with anti-My ␤GTH II or anti-My ␣,␤GTH II serum preabsorbed with the My ␣GTH-subunit. All of the cell forms described for gonadotropes and thyrotropes were also found in a state of involution.Conclusions: Gonadotropes that are of a single morphological type but that vary in ultrastructure are present in the pituitary of captive M. yellowtail. GTH II-and putative GTH I-producing cells were distinguishable from one another and from TSH cells by their different reactions to anti-My ␣,␤GTH II, anti-My ␤GTH II, and anti-My ␣,␤GTH II preabsorbed with the My ␣GTH-subunit.

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Immunocytochemical and ultrastructural characterization of prolactin, growth hormone, and somatolactin cells from the Mediterranean yellowtail (Seriola dumerilii, Risso 1810)

Cited by 18 publications

References 34 publications

Immunocytochemical and Ultrastructural Identification of Pituitary Cell Types in the Protogynous Thalassoma duperrey during Adult Sexual Ontogeny

Immunocytochemical and Ultrastructural Identification of Pituitary Cell Types in the Protogynous Thalassoma duperrey during Adult Sexual Ontogeny

Immunocytochemical and ultrastructural characterization of melanotropin and adrenocorticotropin cells from the mediterranean yellowtail (Seriola dumerilii, Risso 1810)

Gonadotropic and thyrotropic cells from the Mediterranean Yellowtail (Seriola dumerilii; Risso, 1810): Immunocytochemical and ultrastructural characterization

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