Immunocytochemical Characterization of the Pancreatic Islet Cells of the Nile Tilapia (Oreochromis niloticus)

Yang, Hua; Morrison, Carol M.; Conlon, J. Michael; Laybolt, K.; Wright, James R.

doi:10.1006/gcen.1998.7234

Cited by 37 publications

(31 citation statements)

References 30 publications

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“…In 1 dph tilapia larvae the proportion of SST-14-and SST-25-positive cells appeared to be higher than the 21.8% and 23.1% of total immunopositive cells found in the adult (Yang et al, 1999). The cells that stained less intensely for each individual hormone might be pluripotent at this early stage in development, producing more than one hormone.…”

Section: Discussionmentioning

confidence: 70%

“…The number of islets was counted in each serially sectioned specimen. Histological sections were routinely stained with hematoxylin and eosin (H&E), but in some instances they were stained by immunoperoxidase using primary antibodies for the endocrine islet hormones insulin, SST-14, SST-25 (which cross-reacts with the SST-28 found in tilapia), glucagon, or PYY, as in a previous study (Yang et al, 1999).…”

Section: Methodsmentioning

confidence: 99%

“…In mature Nile tilapia, Oreochromis niloticus, as in many other teleosts, much of the endocrine pancreas is consolidated into large, grossly visible islets known as Brockmann bodies, which are enveloped by a thin layer of exocrine pancreas and embedded in adipose connective tissue (Yang et al, 1999). The largest or principal islet (PI) is found on the surface of the gallbladder and is closely associated with several smaller islets.…”

mentioning

confidence: 99%

“…Islets also extend along the bile duct. Insulin-producing cells closely associated with cells producing somatostatin (SST)-14 are usually centrally located in mammalian islets, whereas in teleost fish they are distributed in clusters throughout the islets (Yang et al, 1999). Cells producing "large" somatostatins with 22-28 amino acid residues are found in teleosts, and in tilapia the clusters of insulin and SST-14-positive cells are surrounded by SST-28-producing cells, which are immunopositive to salmon SST-25 antisera (Yang et al, 1999).…”

mentioning

confidence: 99%

“…Insulin-producing cells closely associated with cells producing somatostatin (SST)-14 are usually centrally located in mammalian islets, whereas in teleost fish they are distributed in clusters throughout the islets (Yang et al, 1999). Cells producing "large" somatostatins with 22-28 amino acid residues are found in teleosts, and in tilapia the clusters of insulin and SST-14-positive cells are surrounded by SST-28-producing cells, which are immunopositive to salmon SST-25 antisera (Yang et al, 1999). There are elongate glucagon-positive cells throughout the islets, whereas most peptide tyrosinetyrosine (PYY)-positive cells (pancreatic polypeptide, or PP, arose in tetrapods as a result of duplication of the PYY gene) are peripheral in distribution (Yang et al, 1999).…”

mentioning

confidence: 99%

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Development of the islets, exocrine pancreas, and related ducts in the Nile tilapia, Oreochromis niloticus (Pisces: Cichlidae)

Morrison

Pohajdak

Tam

et al. 2004

Journal of Morphology

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Pancreatic development and the relationship of the islets with the pancreatic, hepatic, and bile ducts were studied in the Nile tilapia, Oreochromis niloticus, from hatching to the onset of maturity at 7 months. The number of islets formed during development was counted, using either serial sections or dithizone staining of isolated islets. There was a general increase in islet number with both age and size. Tilapia housed in individual tanks grew more quickly and had more islets than siblings of the same age left in crowded conditions. The pancreas is a compact organ in early development, and at 1 day posthatch (dph) a single principal islet, positive for all hormones tested (insulin, SST-14, SST-28, glucagon, and PYY), is partially surrounded by exocrine pancreas. However, the exocrine pancreas becomes more disseminated in older fish, following blood vessels along the mesenteries and entering the liver to form a hepatopancreas. The epithelium of the pancreatic duct system from the intercalated ducts to the main duct entering the duodenum was positive for glucagon and SST-14 in 8 and 16 dph tilapia. Individual insulin-immunopositive cells were found in one specimen. At this early stage in development, therefore, the pancreatic duct epithelial cells appear to be pluripotent and may give rise to the small islets found near the pancreatic ducts in 16-37 dph tilapia. Glucagon, SST-14, and some PPY-positive enteroendocrine cells were present in the intestine of the 8 dph larva and in the first part of the intestine of the 16 dph juvenile. Glucagon and SST-14-positive inclusions were found in the apical cytoplasm of the mid-gut epithelium of the 16 dph tilapia. These hormones may have been absorbed from the gut lumen, since they are produced in both the pancreatic ducts and the enteroendocrine cells. At least three hepatic ducts join the cystic duct to form the bile duct, which runs alongside the pancreatic duct to the duodenum.

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Section: Discussionmentioning

confidence: 70%

Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

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Development of the islets, exocrine pancreas, and related ducts in the Nile tilapia, Oreochromis niloticus (Pisces: Cichlidae)

Morrison

Pohajdak

Tam

et al. 2004

Journal of Morphology

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Histological study of the development of the embryo and early larva ofOreochromis niloticus (Pisces: Cichlidae)

2001

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The developmental stages of Oreochromis niloticus are similar to those described in other mouthbreeding tilapias except that, as in zebrafish, no cavity was found in the blastula. Variation in the rate of development of the embryo and larva of O. niloticus was found within a clutch of eggs as well as between clutches. Hatching glands are described for the first time in tilapias. They are widely distributed within the ectoderm covering the head, body, tail, and surface of the yolk sac near its attachment to the embryo. Timing of larval development is similar to that in other mouthbrooding tilapias, but is slower than that found in substrate-spawning tilapias. A pneumatic duct connects the swimbladder to the digestive tract and swimbladder inflation and initiation of feeding occurs at about the same time. The digestive tract of the larva 8 and 9 days after fertilization is similar to that found in the adult, except that there are no digestive glands. An endocrine pancreatic islet was first seen 76 h after fertilization. A prominent thymus gland is present at 100 h. Hematopoietic tissue develops in the vicinity of the pronephros during early larval development. A spleen develops later, 7 days after fertilization.

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Streptozotocin Dose–Response Curve in Tilapia, a Glucose-Responsive Teleost Fish

Wright

Abraham

Dickson

et al. 1999

General and Comparative Endocrinology

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Immunocytochemical Characterization of the Pancreatic Islet Cells of the Nile Tilapia (Oreochromis niloticus)

Cited by 37 publications

References 30 publications

Development of the islets, exocrine pancreas, and related ducts in the Nile tilapia, Oreochromis niloticus (Pisces: Cichlidae)

Development of the islets, exocrine pancreas, and related ducts in the Nile tilapia, Oreochromis niloticus (Pisces: Cichlidae)

Histological study of the development of the embryo and early larva ofOreochromis niloticus (Pisces: Cichlidae)

Streptozotocin Dose–Response Curve in Tilapia, a Glucose-Responsive Teleost Fish

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