Immunocytochemical detection of brain neurons using the selective marker NeuN

Коржевский, Д. Э.; Gilerovich, E. G.; Zin’kova, N. N.; Grigor’ev, I. P.; Otellin, V. A.

doi:10.1007/s11055-006-0098-5

Cited by 19 publications

(20 citation statements)

References 3 publications

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“…To identify the cell types that were transfected by the adenovirus used in the present study, we performed double immunohistochemical staining for ␤-galactosidase and a neuronal marker (NeuN; Chemicon International Inc). 22 Western blot analysis was performed using rabbit anti-SOD-1 polyclonal IgG (1:10 000, Santa Cruz Biotechnology), mouse antihemagglutinin (HA) monoclonal IgG (1:10 000, Sigma), or rabbit anti-␤ tubulin polyclonal IgG (1:10 000, Santa Cruz Biotechnology).…”

Section: Analysis Of Gene Expressionmentioning

confidence: 99%

Inhibition of Rac1-Derived Reactive Oxygen Species in Nucleus Tractus Solitarius Decreases Blood Pressure and Heart Rate in Stroke-Prone Spontaneously Hypertensive Rats

et al. 2007

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Abstract-Reactive oxygen species (ROS) in the brain are thought to contribute to the neuropathogenesis of hypertension by enhancing sympathetic nervous system activity. The nucleus tractus solitarius (NTS), which receives afferent input from baroreceptors, has an important role in cardiovascular regulation. reduced nicotinamide-adenine dinucleotide phosphate oxidase is thought to be a major source of ROS in the NTS. Rac1 is a small G protein and a key component of reduced nicotinamide-adenine dinucleotide phosphate oxidase. The role of Rac1-derived ROS in the NTS in cardiovascular regulation of hypertension is unknown. Therefore, we examined whether inhibition of Rac1 in the NTS decreases ROS generation, thereby reducing blood pressure in stroke-prone spontaneously hypertensive rats (SHRSPs).The basal Rac1 activity level in the NTS was greater in SHRSPs than in Wistar-Kyoto rats. Inhibition of Rac1, induced by transfecting adenovirus vectors encoding dominant-negative Rac1 into the NTS, decreased blood pressure, heart rate, and urinary norepinephrine excretion in SHRSPs but not in Wistar-Kyoto rats. Inhibition of Rac1 also reduced nicotinamide-adenine dinucleotide phosphate oxidase activity and ROS generation. In addition, Cu/Zn-superoxide dismutase activity in the NTS of SHRSPs was decreased compared with that of Wistar-Kyoto rats, despite the increased ROS generation. Overexpression of Cu/Zn-superoxide dismutase in the NTS decreased blood pressure and heart rate in SHRSPs. These results indicate that the activation of Rac1 in the NTS generates ROS via reduced nicotinamide-adenine dinucleotide phosphate oxidase in SHRSPs, and this mechanism might be important for the neuropathogenesis of hypertension in SHRSPs. Key Words: blood pressure Ⅲ heart rate Ⅲ sympathetic nervous system Ⅲ hypertension Ⅲ brain T here is accumulating evidence that reactive oxygen species (ROS) in the cardiovascular regulatory nuclei in the brain have a crucial role in blood pressure regulation in hypertension via modulating the sympathetic nervous system. 1-5 Reduced nicotinamide-adenine dinucleotide phosphate [NAD(P)H] oxidase is a major source of ROS in hypertension 6 and has a critical role in generating ROS in the brain. 2,5,7 Rac1 is a small G protein that is an important signaling molecule involved in integrating intracellular transduction pathways toward NAD(P)H oxidase activation. 2,8,9 Rac1 requires lipid modifications to migrate from the cytosol to the plasma membrane, which is a necessary step for activating the ROSgenerating NAD(P)H oxidase enzyme system. 8,9 The nucleus tractus solitarius (NTS) in the brain stem has an important role in cardiovascular regulation. 10 -16 The NTS receives afferent input from baroreceptors and chemoreceptors 12 and has reciprocal interconnections with other nuclei involved in central autonomic regulation. 17 In addition, the essential NAD(P)H oxidase subunit gp91 phox is present in somatodendritic and axonal profiles that contain angiotensin II (Ang II) subtype 1 receptors in the NTS, and Ang I...

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Section: Analysis Of Gene Expressionmentioning

confidence: 99%

Inhibition of Rac1-Derived Reactive Oxygen Species in Nucleus Tractus Solitarius Decreases Blood Pressure and Heart Rate in Stroke-Prone Spontaneously Hypertensive Rats

et al. 2007

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“…The samples were obtained during elective biopsy and had no signs of autolysis. They were fixed with 2% formalin in 80% ethanol, 10% formalin, and zinc-ethanol-formaldehyde [2]. After fixation, the samples were dehydrated and embedded in paraffin.…”

Section: Methodsmentioning

confidence: 99%

Expression of Neural Stem Cell Marker Nestin in the Kidney of Rats and Humans

Кирик

Коржевский

2009

Bull Exp Biol Med

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“…Fragments of the dorsolateral wall of the anterior cerebral vesicle were then transplanted into the sciatic nerve of adult rats under ether narcosis [8]. After 1, 3, 7, and 10 days postoperation the sciatic nerves of the recipient rats with the transplants were fixed in Bouin's solution for histological studies and in ethanol-formalin for immunohistochemical reaction for detection of NeuN neuronal nuclear protein [3]. Paraffin sections (5 µ) were stained with hematoxylin and eosin.…”

Section: Methodsmentioning

confidence: 99%

Serotonin is involved in the regulation of histogenetic processes in rat embryonic neocortex

Петрова

Otellin

2007

Bull Exp Biol Med

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We compared the dynamics of the development of ectopic transplants of embryonic (day 14) primordial neocortex from rats injected with serotonin inhibitor (para-chlorophenylalanine; 400 mg/kg) on day 11 of pregnancy and transplants of similar primordial neocortex incubated before transplantation in a medium with serotonin (3 microg/ml). The study of mitotic activity and differentiation of transplanted cells showed that serotonin promoted survival of the transplanted neuroepithelial cells and their differentiation into nerve cells, and is involved in the regulation of their proliferation. We hypothesized that serotonin accelerated the cell cycle of transplanted cells, thus accelerating the neuron differentiation.

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Immunocytochemical detection of brain neurons using the selective marker NeuN

Cited by 19 publications

References 3 publications

Inhibition of Rac1-Derived Reactive Oxygen Species in Nucleus Tractus Solitarius Decreases Blood Pressure and Heart Rate in Stroke-Prone Spontaneously Hypertensive Rats

Inhibition of Rac1-Derived Reactive Oxygen Species in Nucleus Tractus Solitarius Decreases Blood Pressure and Heart Rate in Stroke-Prone Spontaneously Hypertensive Rats

Expression of Neural Stem Cell Marker Nestin in the Kidney of Rats and Humans

Serotonin is involved in the regulation of histogenetic processes in rat embryonic neocortex

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