Immunocytochemical detection of CA 125 and EMA in ovarian serous cystadenocarcinomas by acitic cytology.

Shigemasa, Kazushi; Tanioka, Yoshihide; Matsuda, Hiroshi; Nagai, Nobuo; Egawa, Kenji; Katsube, Yukiteru; Uebaba, Yoshimi; Fujiwara, Atsushi

doi:10.5795/jjscc.27.459

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“…Application of immunohistochemical staining to decolored Papanicolaou preparations has been reported by many researchers, 13,14 confirming the existence of the antigen. In the present study, it was confirmed that application of immunohistochemical techniques makes possible the observation of the membrane filter, as is the case with the conventional preparation.…”

Section: Membrane Filter Methodsmentioning

confidence: 81%

Usefulness of Auto Cyto Fix (membrane filter method) for the application of immunohistochemistry

Iijima

Shiina

Ohkoudo

et al. 2002

Diagnostic Cytopathology

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We developed the Auto Cyto Fix (ACF) 1000 as an experimental model, which was adapted as a membrane filter method for automatically making smear preparations fixed with 95% ethanol. In this study, immunohistochemistry was applied to the cells smeared on the membrane filter, and effectiveness was examined. Twenty-four effusions and HeLa 229 cells were used. These cell samples were produced by the ACF1000 on the membrane filter and stained by the Papanicolaou method. After observation, these were decolored and stained by immunohistochemical techniques. Antibodies included CEA, EMA, and MIB-1. CEA and EMA were detected by the indirect method, and were colored by DAB, AEC, and new fuchsin. MIB-1 was detected by the immunoperoxidase (LSAB) and the immunofluorescence method. A uniformly stabilized immunoreaction was obtained and was equivalent to or better than that of the conventional technique. In addition, there was no background staining on the membrane filter. Based on these results, the membrane filter preparation produced by the ACF1000 was also effectively applied to immunohistochemical observations.

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Section: Membrane Filter Methodsmentioning

confidence: 81%