Immunocytochemical examination of immune cells in periapical granulomata and odontogenic cysts

Abstract

AT. Immunocytochemical examination of immune cells in periapical granulomata and odontogenic cysts. J Oral Pathol 1988: 17: 84-90. Monoclonal antibodies (mAbs) were used to determine the presence and distribution of immune cells including lymphocytes, macrophages and Langerhans cells, in normal periodontal ligament, periapical granulomata, periapical cysts and dental developmental cysts. Isolated T-lymphocytes, but not B-lymphocytes, were detected in specimens of non-inflamed periodontal ligament. Increased numbers of T and B lymphocytes were found in all of the lesions examined. Monocytes/maerophages were associated with most periapical granulomata, dental developmental cysts and all periapical cysts. Langerhans cells, intraepithelial lymphocytes, and monocytes/maerophages were not detected in the rests of Malassez but were found in some epithelia within periapical granulomata and in most epithelial linings of odontogenic cysts. Increased numbers of immune cells were seen around proliferative epithelia and adjacent to the epithelial linings of cysts. Epithelium, particularly that of odontogenic cysts, showed positive reactions for HLA-Dr, lysozyme and for a-1 antitrypsin. The presence of immune cells in periapical granulomata and odontogenic cysts, suggests that cell-mediated and humoral immunoreactions occur in these lesions and may be assoeiated with the epithelial proliferation within the periapical lesions.

Epithelial Cell Rests of Malassez and OX6‐Immunopositive Cells in the Periodontal Ligament of Rat Molars: A Light and Transmission Electron Microscope Study

“…These results show that the epithelial cells of ERM and the surrounding immunopositive cells most probably communicate with each other, as well as with the surrounding fibroblasts (Gao et al, 1988;Má rton et al, 1998;Götz et al, 2003;Shimonishi et al, 2005). The epithelial-mesenchymal interaction indicated in the present study suggests a functional property for the cells comprising the ERM clusters.…”

“…The epithelial-mesenchymal interaction indicated in the present study suggests a functional property for the cells comprising the ERM clusters. Cementum formation and repair (Brice et al, 1991;Hasegawa et al, 2003), as well as immune-defense properties and formation of periapical granulomata and odontogenic cysts (Gao et al, 1988) have been reported previously.…”

Increased Gene Expression of Toll-like Receptors and Antigen-Presenting Cell–related Molecules in the Onset of Experimentally Induced Furcation Lesions of Endodontic Origin in Rat Molars

“…The up-regulation of class II MHC correlates with APC activity (25,26). In humans, CD83 expression is a hallmark of mature DCs (27) and activated DCs (28). CD86 (B7-2) plays a major role in T-cell activation (29).…”

IL-1α and TNF-α Expression in Early Periapical Lesions of Normal and Immunodeficient Mice

“…Introduction T-and B-lymphocytes comprise a large proportion of inflammatory cells in human periapical lesions (Cymerman et al, 1984;Nilsen et al, 1984;Torabinejad and Kettering, 1985;Gao et al, 1988;Lukic et al, 1990). Furthermore, Tlymphocyte presence in periapical lesions correlates with lesion size (Matsuo et al, 1992).…”