Immunocytochemical identification and localization of the different cell types in the pituitary of the seabass (Dicentrarchus labrax)

Cambre, M; Verdonck, W; Ollevier, Frans; Vandesande, Frans; Batten, Trevor; Kühn, Eduard

doi:10.1016/0016-6480(86)90222-4

Cited by 52 publications

(17 citation statements)

References 13 publications

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“…The MSH immunoreactive cells were found in the PI of A. gigas surrounding the neurohypophysial tissue intermingled with the SL cells, as with the typical distribution of these cells reported for many teleosts (Batten 1986;Cambré et al 1986;Quesada et al 1988;Rodríguez-Gómez et al 2001;Vissio et al 1997). However, as an arrangement not commonly found among teleosts, these cells were also in clusters, forming a net with their cytoplasmic processes in contact with neighboring cells, and also surrounding blood vessels.…”

Section: Structure Of the Pituitary Glandmentioning

confidence: 78%

Immunocytochemical identification of adenohypophyseal cells in the pirarucu (Arapaima gigas), an Amazonian basal teleost

Borella

Venturieri

Mancera

2008

Fish Physiol Biochem

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The adenohypophysis (AH) of juvenile pirarucu (Arapaima gigas), a representative species of the Osteoglossomorpha (bonytongue fishes, one of the oldest living groups of the teleosts), was studied using histochemical and immunocytochemical methods. The AH is comprised of the pars distalis (PD), without a clear distinction between rostral pars distalis (RPD) and proximal pars distalis (PPD), and the pars intermedia (PI). The neurohypophysis (NH) is positioned on top of the PD and penetrates and branches into the PI. In the most rostral dorsal portion of the PD, adrenocorticotropic cells and fusiform gonadotropic cells were found. In the central PD, scarce prolactin-producing cells and growth-hormone-producing cells were located mainly in the dorsal part, whereas round gonadotropic cells were abundant in the ventral portion of this region. Human thyrotropin immunoreactive cells were not found in the entire AH. In the PI, melanotropic, some adrenocorticotropic, and somatolactin-producing cells were located intermingled surrounding the neurohypophyseal branches. Our results showed that the A. gigas pituitary has some basal characteristics between the ancient Actinopterygii and the more derived teleosts.

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Section: Structure Of the Pituitary Glandmentioning

confidence: 78%

Immunocytochemical identification of adenohypophyseal cells in the pirarucu (Arapaima gigas), an Amazonian basal teleost

Borella

Venturieri

Mancera

2008

Fish Physiol Biochem

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“…Antisera against mammalian LH have been widely used to study the gonadotropic hormone-secreting cells in the pituitary of teleosts. Whereas the antisera against bovine-LH were employed to label the gonadotrops in the PPD of the teleost Barbus barbus (Toubeau et al, 1991), those in the PPD of Dicentrarchus labrax (Cambré et al, 1986) and Austromenidia laticlavia (Siegmund et al, 1987) were labeled with anti-human LH antisera. Anti- The Journal of Comparative Neurology.…”

Section: Discussionmentioning

confidence: 99%

Reproduction phase‐related variations in neuropeptide Y immunoreactivity in the olfactory system, forebrain, and pituitary of the female catfish, Clarias batrachus (Linn.)

Mazumdar

Sakharkar

Singru

et al. 2007

J of Comparative Neurology

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The aim of this study was to determine whether neuropeptide Y (NPY) immunoreactivity in the cells and fibers in the forebrain and pituitary of Clarias batrachus is linked to the annual reproductive cycle. A steady rise in luteinizing hormone (LH) immunoreactivity was seen in the pituitary through preparatory (February-April) and prespawning (May-June) phases; it was greatly reduced during spawning (July-August; P < 0.001) and partially replenished during postspawning (September-November; P < 0.01) through resting (December-January) phases. Although NPY immunoreactivity in olfactory receptor neurons and olfactory nerve layer in olfactory bulb was gradually augmented during resting through prespawning phases (P < 0.001), attaining a peak in spawning phase (P < 0.001), a dramatic decline was encountered during postspawning phase (P < 0.001). A similar pattern was also observed in NPY-containing fibers of the medial olfactory tract (MOT) and pituitary. However, a different pattern of NPY immunoreactivity was observed in the neurons of nucleus entopeduncularis (NE) and nucleus preopticus periventricularis (NPP). Whereas these neurons and fibers in the forebrain showed significant augmentation during the resting through prespawning phases (P < 0.001), the immunoreactivity dramatically declined during spawning (P < 0.001) and was partially replenished in the postspawning phase. Testosterone injection of juveniles significantly augmented (P < 0.001) NPY immunoreactivity in NE neurons. We suggest that NPY cells of NE and NPP, and related fiber systems, might be involved in processing of sex steroid-borne information and regulation of the gonadotropin-releasing hormone-LH axis.

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“…In A. naccarii we used the human ACTH (1-24) antiserum, largely used to detect ir-ACTH cells in several teleosts (Follenius and Dubois 1980;Batten 1986;CambrØ et al 1986;García-Hernµndez et al 1996;Vissio et al 1997), although unable to identify the ir-MSH ones. The combined use of human ACTH and a-MSH antisera allows a clear identification of the two cell types during A. naccarii development, as previously obtained in adult A. baeri and A. transmontanus (Pelissero et al 1988;Joss et al 1990A) and in teleosts (Batten 1986;CambrØ et al 1986;García-Hernµndez et al 1996;Villaplana et al 2002;Grandi et al 2003).…”

Section: Discussionmentioning

confidence: 99%

Early development of the pituitary gland in Acipenser naccarii (Chondrostei, Acipenseriformes): an immunocytochemical study

Grandi

Chicca

2004

Anat Embryol

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The distribution and appearance of secretory cells in the pituitary gland were investigated for the first time in a chondrostean species, Acipenser naccarii, from embryos to juveniles, by immunohistochemistry with mammalian and teleost hormone antisera. On 5.5 day post-fertilization (2.5 days pre-hatching), the pituitary of embryos appears as an oval cell mass with a narrow central cavity (hypophysial cleft), close to the ventral border of diencephalon under the third ventricle. At that time no neurohypophysis is observed, the adenohypophysis is not yet structurally divided into pars intermedia (PI) and pars distalis (PD) and only immunoreactive growth hormone cells are detectable. Seven days post-fertilization (1 day pre-hatching) the immunoreactive thyrotropic cells appear in the ventral region and the immunoreactive adrenocorticotropic cells in the posterior dorsal one. At hatching, some immunoreactive melanotropic (ir-MSH) cells are visible in the posterior dorsal region and some immunoreactive prolactin cells in the anterior one. Eight days later the immunoreactive somatolactin cells appear along the posterior dorsal border and the immunoreactive gonadotropic I (ir-GtH I) cells in the ventral region. Here, a few ir-GtH II cells finally appear in 76-86 day old juveniles. The gland elongates after hatching and in 8-day-old larvae two adenohypophysial regions are identified: a posterior (the presumptive PI) and an anterior one (the presumptive PD). In 156-166-day-old juveniles three regions (rostral and proximal pars distalis and pars intermedia) appear and a high number of ir-MSH cells are visible in the rostral region. The first protrusion of neurohypophysis into adenohypophysis is observed in 76-86-day-old juveniles and increases with age, branching into PI. The rostro-caudal distribution of the immunoreactive cells follows the spatial expression of the corresponding hormone gene families observed in zebra fish, suggesting similar differentiating mechanisms in teleosts and chondrosteans.

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Immunocytochemical identification and localization of the different cell types in the pituitary of the seabass (Dicentrarchus labrax)

Cited by 52 publications

References 13 publications

Immunocytochemical identification of adenohypophyseal cells in the pirarucu (Arapaima gigas), an Amazonian basal teleost

Immunocytochemical identification of adenohypophyseal cells in the pirarucu (Arapaima gigas), an Amazonian basal teleost

Reproduction phase‐related variations in neuropeptide Y immunoreactivity in the olfactory system, forebrain, and pituitary of the female catfish, Clarias batrachus (Linn.)

Early development of the pituitary gland in Acipenser naccarii (Chondrostei, Acipenseriformes): an immunocytochemical study

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