Immunocytochemical localisation of GABA in endocrine cells of the rat entero-pancreatic system

Gilon, Patrick

doi:10.1016/0248-4900(88)90021-4

Cited by 8 publications

(16 citation statements)

References 18 publications

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“…The presence of GABA-immunoreactivity and GABA uptake in endocrine cells of the rat stomach is in good agreement with other studies, revealing immunostaining and autoradiographic labelling in endocrine cells of the duodenum and epithelial cells of the gastro-intestinal tract (Gilon et al 1987b(Gilon et al , 1988Jessen et al 1988 ;Davanger et al 1989). The majority of these cells in the gastric epithelium lack any contact with the lumen of the glands.…”

Section: Immunocytochemical and Autoradiographic Localization Of Gabasupporting

confidence: 91%

“…The fact that GABA-immunoreactivity was heterogeneous in different nuclei could result from differential accessibility of the nuclear antigenic sites to the antibodies in the thick Vibratome sections. The GABA-immunoreactivity in the nucleus correlates with the autoradiographic labelling in this structure and with experiments performed on nerve cells, pancreas and duodenum with a similar antiserum (Gamrani et al 1986;Gilon et al 1988). (Figs.…”

Section: Immunocytochemical and Autoradiographic Localization Of Gabasupporting

confidence: 74%

“…This antiserum has already been used with success in previous experiments on rat pancreas and duodenum (Gilon et al 1988). In the present study, the specificity of the immunocytochemical reaction was confirmed on brain and stomach sections either by omitting the primary antibody or by preincubating the antibody in the presence of the GABA-and fl-alanine-glutaraldehyde reduced conjugates.…”

Section: Immunocytochemical and Autoradiographic Localization Of Gabasupporting

confidence: 74%

“…In many cases however, the granules of the grain-labelled cells were quite smaller (250 nm) than those of the immunoreactive cells, suggesting that cells taking up GABA are not necessarily those containing it. This possibility is supported by the example of the endocrine pancreas: the insulin cells possess high level of GABA but are unable to take it up, whereas the somatostatin cells possess a high affinity uptake of GABA but do not contain it (Okada 1986;Gilon et al 1988Gilon et al , 1989. In this view, immunocytochemistry against the enzymes of synthesis (GAD) or degradation (GABA-T) of GABA coupled to autoradiography would be helpful in solving this problem.…”

Section: Immunocytochemical and Autoradiographic Localization Of Gabamentioning

confidence: 99%

“…In these organs, several categories of endocrine cells feature as well morphological as physiological indications of direct interaction with GABA, i.e. prolactin- (Erd6 1985;Anderson and Mitchell 1986;McCann and Rettori 1986;Melis et al 1986), insulin-, and somatostatin-secreting cells (Okada 1986;Garry et al 1987a;Gilon et al 1987aGilon et al , 1988Gilon and Remacle 1989). Moreover, certain of them have been reported to contain GABA: the * To whom offprints requests should be sent insulin-secreting cells of the pancreatic islets possess as well the ?-aminoacid as the enzymes of its synthesis (GAD) and degradation (GABA-T) (Vincent et al 1983;Okada 1986;Wu et al 1986;Garry et al 1987b); endocrine cells of the rat duodenum were also found to be immunoreactive to anti-GABA antibodies (Gilon et al 1988).…”

Section: Introductionmentioning

confidence: 99%

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Immunocytochemical and autoradiographic studies of the endocrine cells interacting with GABA in the rat stomach

et al. 1990

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There are now increasing evidences suggesting that GABA is able of direct interaction with certain endocrine cells. In the present study, highly specific anti-GABA-glutaraldehyde antibodies and 3H-GABA uptake were used at the light and electron microscope levels to investigate the occurrence of cells containing endogenous GABA or taking up exogenous GABA in the mucosal antrum and corpus of the rat stomach. Only certain endocrine cell types of both regions were immunostained or grain-labelled. However, the morphology of their secretory granules did not allow to identify the nature of their hormone with certainty but suggested that somatostatin-like cells could interact with GABA. The combination of gastrin and somatostatin immunodetection with 3H-GABA uptake autoradiography at the light microscope level, revealed that a subpopulation of somatostatin-like cells and other still unidentified endocrine cells are able to take up GABA, while the gastrin-like cells are not. These results rei... Summary. There are now increasing evidences suggesting that GABA is able of direct interaction with certain endocrine cells. In the present study, highly specific anti-GABAglutaraldehyde antibodies and aH-GABA uptake were used at the light and electron microscope levels to investigate the occurrence of cells containing endogenous GABA or taking up exogenous GABA in the mucosal antrum and corpus of the rat stomach. Only certain endocrine cell types of both regions were immunostained or grain-labelled. However, the morphology of their secretory granules did not allow to identify the nature of their hormone with certainty but suggested that somatostatin-like cells could interact with GABA. The combination of gastrin and somatostatin immunodetection with aH-GABA uptake autoradiography at the light microscope level, revealed that a subpopulation of somatostatin-like cells and other still unidentified endocrine cells are able to take up GABA, while the gastrin-like cells are not. These results reinforce the hypothesis that certain endocrine cell types of the diffuse endocrine system of the digestive tract are able to directly interact with GABA.

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Section: Immunocytochemical and Autoradiographic Localization Of Gabasupporting

confidence: 91%

Section: Immunocytochemical and Autoradiographic Localization Of Gabasupporting

confidence: 74%

Section: Immunocytochemical and Autoradiographic Localization Of Gabasupporting

confidence: 74%

Section: Immunocytochemical and Autoradiographic Localization Of Gabamentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Immunocytochemical and autoradiographic studies of the endocrine cells interacting with GABA in the rat stomach

et al. 1990

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Physiological and Pathophysiological Control of Glucagon Secretion by Pancreatic α-Cells

Gilon¹,

Cheng-Xue²,

Lai³

et al. 2014

Islets of Langerhans

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Gamma-aminobutyric acid up- and downregulates insulin secretion from beta cells in concert with changes in glucose concentration

et al. 2006

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Aims/hypothesis: The role of gamma-aminobutyric acid (GABA) and A-type GABA receptors (GABA A Rs) in modulating islet endocrine function has been actively investigated since the identification of GABA and GABA A Rs in the pancreatic islets. However, the reported effects of GABA A R activation on insulin secretion from islet beta cells have been controversial. Methods: This study examined the hypothesis that the effect of GABA on beta cell insulin secretion is dependent on glucose concentration. Results: Perforated patchclamp recordings in INS-1 cells demonstrated that GABA, at concentrations ranging from 1 to 1,000 μmol/l, induced a transmembrane current (I GABA ) which was sensitive to the GABA A R antagonist bicuculline. The current-voltage relationship revealed that I GABA reversed at −42±2.2 mV, independently of glucose concentration. Nevertheless, the glucose concentration critically controlled the membrane potential (V M ), i.e., at low glucose (0 or 2.8 mmol/l) the endogenous V M of INS-1 cells was below the I GABA reversal potential and at high glucose (16.7 or 28 mmol/l), the endogenous V M of INS-1 cells was above the I GABA reversal potential. Therefore, GABA dose-dependently induced membrane depolarisation at a low glucose concentration, but hyperpolarisation at a high glucose concentration. Consistent with electrophysiological findings, insulin secretion assays demonstrated that at 2.8 mmol/l glucose, GABA increased insulin secretion in a dose-dependent fashion (p<0.05, n=7). This enhancement was blocked by bicuculline (p<0.05, n=4). In contrast, in the presence of 28 mmol/l glucose, GABA suppressed the secretion of insulin (p<0.05, n=5). Conclusions/interpretation: These findings indicate that activation of GABA A Rs in beta cells regulates insulin secretion in concert with changes in glucose levels.

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Immunocytochemical localisation of GABA in endocrine cells of the rat entero-pancreatic system

Cited by 8 publications

References 18 publications

Immunocytochemical and autoradiographic studies of the endocrine cells interacting with GABA in the rat stomach

Immunocytochemical and autoradiographic studies of the endocrine cells interacting with GABA in the rat stomach

Physiological and Pathophysiological Control of Glucagon Secretion by Pancreatic α-Cells

Gamma-aminobutyric acid up- and downregulates insulin secretion from beta cells in concert with changes in glucose concentration

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