Immunocytochemical localization of allergenic proteins from mature to activated Zygophyllum fabago L. (Zygophyllaceae) pollen grains

Castells, Teresa; Arcalís, Elsa; Moreno‐Grau, Stella; Bayo, Javier; Elvira‐Rendueles, Belén; Belchi, J.; Seoane-Camba, Juan; Suárez‐Cervera, María

doi:10.1078/0171-9335-00223

Cited by 22 publications

(20 citation statements)

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“…Different processes that occur in the anther and pollen grains can explain the presence of other allergenic particles in the atmosphere. During germination processes, the pollen hydration facilitates the elution of allergenic proteins in just a few minutes (Casas et al 1996;Castells et al 2002;Suárez-Cervera et al 2003;Suárez-Cervera and Seoane-Camba 2005;Vega-Maray et al 2004). Allergens can be extruded from pollen grains in wet conditions similar to the in vitro and in vivo germination processes (Suárez-Cervera et al 2003;Suárez-Cervera and SeoaneCamba 2005).…”

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confidence: 97%

The combination of airborne pollen and allergen quantification to reliably assess the real pollinosis risk in different bioclimatic areas

et al. 2010

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Exposure to allergens represents a key factor among the environmental determinants of asthma. The most common information available for pollinosis patients is the concentration of pollen grains in the bioaerosol and their temporal distribution. However, in recent years, discordance between pollen concentrations and allergic symptoms has been detected. The purpose of this research is to evaluate the relationship between pollen counts and the atmospheric aeroallergen concentrations in different Spanish bioclimatic areas. For the monitoring of allergen content in the air, a quantitative antigen-antibody technique combined with the Cyclone sampling methodology was used. The study was conducted during 2007 by considering some of the most common allergens that induce pollinosis in each area: Platanus and Urticaceae in Ourense and Cartagena, and Poaceae in Ourense and León. In Ourense, pollen counts and aeroallergen concentrations coincided for the three pollen types studied, and the pollen and allergen data associated with the meteorological factors were highly significant for the pollen counts. In Cartagena (for Platanus and Urticaceae) and León (for Poaceae), the low correlations between pollen counts and allergen concentrations obtained could be due to the specific bioclimatic conditions. In contrast, the higher allergen concentrations found in the atmosphere in Cartagena and León compared to Ourense could be related to the existing pollutant levels there, inducing a higher expression of plant pathogenesis-related proteins in the plants of polluted cities. The combination of pollen counts and allergen quantification must be assessed to reliably estimate exposure of allergic people to allergens in different bioclimatic areas.

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confidence: 97%

The combination of airborne pollen and allergen quantification to reliably assess the real pollinosis risk in different bioclimatic areas

et al. 2010

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“…The extreme solubility and high accessibility, together with stability and size, are thought to determine the allergenicity of proteins since it enables them to cross the mucosal barriers Aalberse, 2000;Castells et al, 2002). These properties and the resulting mobility and rapid dislocation upon hydration made it difficult to locate the allergens within the pollen grains using conventional aqueous fixation methods (Grote, 1999).…”

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confidence: 99%

Walls of allergenic pollen: Special reference to the endexine

Diethart

Sam

Weber

2007

Grana

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Allergic rhinitis is an increasing health problem and pollen allergens are amongst the main elicitors of hay fever symptoms. Allergenic pollen contains a set of differently allergenic proteins which are thought to play a role in the pollen germination and fertilisation process. They are released upon contact with the stigma or mucosa or upon pollen grain rupture. Although the determinants of allergenicity of these proteins are still largely undiscovered, accessibility and solubility are now thought to mainly influence allergenic potency. Pollen of 61 allergenic plants was investigated with scanning and transmission electron microscopy. Most of the minor allergenic plants like species of the families Salicaceae, Fagaceae or Ulmaceae show the typical pollen wall organisation with intine, compact endexine and ektexine whereas in the majority of the major allergenic plants like species of Betulaceae and Poaceae the endexine is not detectable. Ambrosia artemisiifolia exhibits a laminated endexine. In addition, pollen of these major allergenic plants does not have electron-dense pollenkitt and starch is stored in a high proportion of the examined pollen. The question is raised whether pollen morphology and ultrastructure might contribute to the accessibility and therefore allergenicity of allergenic proteins.

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“…An interesting hypothesis is that the referred stained materials are highly glycosylated proteins (probably gametophytic glycoproteins or most likely their precursors) involved in the pollen-pistil recognition and interaction. It is known that many of these glycoproteins are allergens and some of them were already immunolocalized in the lumen of RER cisternae of Zygophyllum fabago (Castells et al, 2002) and several species of Oleaceae (Rodríguez-García et al, 1995;Alché et al, 2002). The same may be true for Eucalyptus globulus pollen since it is also considered as a potential cause of respiratory allergic diseases (Galdi et al, 2003).…”

Section: Discussionmentioning

confidence: 82%