Immunocytochemical Localization of Angiotensin II and Vasopressin in Rat Hypothalamus: Evidence for Production in the Same Neuron

Kilcoyne, Margaret M.; Hoffman, Donald L.; Zimmerman, Earl A.

doi:10.1042/cs059057s

Cited by 105 publications

(15 citation statements)

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“…Second, cell body staining was also brighter in the subfornical organ [56]. And third, magnocellular neurosecretory neurons of the paraventricular and supraoptic nuclei were, for the most part, not stained in the Brattleboro rat [47,55].…”

Section: Resultsmentioning

confidence: 95%

Organization of Angiotensin II Immunoreactive Cells and Fibers in the Rat Central Nervous System

1985

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The distribution of angiotensin II (AII)-immunoreactive cells and fibers was examined in adult male Sprague-Dawley rats with and without colchicine pretreatment. As seems to be the case for a number of other neuropeptides, AII is preferentially found in brain stem, hypothalamic, and limbic structures involved in the control of homeostatic functions. AII-stained cell bodies were most prominent in magnocellular parts of the paraventricular and supraoptic nuclei, and cells were also found in parvocellular parts of the former. Other hypothalamic nuclei containing cell bodies include the suprachiasmatic nucleus, the medial preoptic area, and perifornical parts of the lateral hypothalamic area. Of considerable interest was robust staining in several of the circumventricular organs, in particular the subfornical organ, where both cells and fibers were found. The results of water deprivation and nephrectomy suggest that this staining does not represent uptake of circulating peptide, but instead, represents AII-containing neural connections. In the thalamus, AII-stained cells were found in the paraventricular nucleus, the central medial nucleus, the nucleus reuniens, and rostral parts of the zona incerta. Two cell groups in the basal telencephalon, in the dorsal part of the bed nucleus of the stria teminalis and in the medial nucleus of the amygdala, lay at either end of an AII-stained pathway coursing through the stria terminalis. In the midbrain, immunoreactive cells were found in the interpeduncular and peripeduncular nuclei, and one pontine cell group was detected in the most lateral part of the lateral parabrachial nucleus. The only AII-stained cells in the medulla were in the nucleus of the solitary tract, near the margin of the area postrema. Fibers were found at all levels of the central nervous system, from the olfactory bulbs to the spinal cord, where terminal fields were observed in the substantia gelatinosa and in the intermediolateral cell column. Longitudinally oriented fibers were present throughout the periventricular fiber system and in the medial forebrain bundle, including its caudal extension in ventrolateral parts of the brain stem. It is suggested that, at many different levels, AIIserves as both a hormone and neurotransmitter for fluid balance.

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Section: Resultsmentioning

confidence: 95%

Organization of Angiotensin II Immunoreactive Cells and Fibers in the Rat Central Nervous System

1985

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“…Neurones of the paraventricular and supraoptic nuclei were unstained (19). In a different study, immunoreactive All was located in cell bodies of magnocellular neurones in the supraoptic and paraventricular nuclei and in parvocellular neurones of the suprachiasmatic nucleus, all of which also contained vasopressin (20). Weyhenmeyer and Phillips (21) found All immunoreactivity in cell bodies of the supraoptic and paraventricular nuclei of the hypothalamus, hippocampus and cortex and fibers in the anterior and middle hypothalamus, basal ganglia, thalamus, locus coeruleus, nucleus of the solitary tract, limbic structures, and reticular formation.…”

Section: Discussionmentioning

confidence: 99%

“…All-containing nerve terminals and neurones have been localized in brain by immunohistochemistry (17)(18)(19)(20)(21)(22). High densities were found in the substantia gelatinosa of the spinal cord and spinal trigeminal nucleus, sympathetic lateral column and medial external layer of the median eminence (17), and hypothalamus (20,22).…”

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confidence: 99%

Autoradiographic localization of angiotensin II receptors in rat brain.

Mendelsohn¹,

Quirion²,

Saavedra³

et al. 1984

Proc. Natl. Acad. Sci. U.S.A.

443

209

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The 125I-labeled agonist analog [1-sarcosine]-angiotensin II ([Sar']AII) bound with high specificity and affinity (Ka = 2 x 109 M-1) to a single class of receptor sites in rat brain. This ligand was used to analyze the distribution of All receptors in rat brain by in vitro autoradiography followed by computerized densitometry and color coding. A very high density of All receptors was found in the subfornical organ, paraventricular and periventricular nuclei of the hypothalamus, nucleus of the tractus solitarius, and area postrema. A high concentration of receptors was found in the suprachiasmatic nucleus of the hypothalamus, lateral olfactory tracts, nuclei of the accessory and lateral olfactory tracts, triangular septal nucleus, subthalamic nucleus, locus coeruleus, and inferior olivary nuclei. Moderate receptor concentrations were found in the organum vasculosum of the lamina terminalis, median preoptic nucleus, medial habenular nucleus, lateral septum, ventroposterior thalamic nucleus, median eminence, medial geniculate nucleus, superior colliculus, subiculum, preand parasubiculum, and spinal trigeminal tract. Low concentrations of sites were seen in caudate-putamen, nucleus accumbens, amygdala, and gray matter of the spinal cord. These studies have demonstrated that All receptors are distributed in a highly characteristic anatomical pattern in the brain. The high concentrations of All receptors at numerous physiologically relevant sites are consistent with the emerging evidence for multiple roles of All as a neuropeptide in the central nervous system.

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“…On the other hand, the SON and the PVN themselves are reported to contain immunoreactive angiotensin II [5,17,20,46] and its receptors [ 12,24,29], Therefore, there is the possibi lity that angiotensin II acts directly on the vasopressinergic magnocellular neurons in the SON and the PVN to stimulate vasopressin release. Indeed, the electrical activity of vasopressi nergic magnocellular neurons in the PVN and SON was stimu lated by local administration of angiotensin II [2,26,30], and vasopressin secretion in the monkey was stimulated by microin jection of angiotensin II into the SON [38].…”

Section: Introductionmentioning

confidence: 99%

Effect on Vasopressin Release of Microinjection of Angiotensin II into the Paraventricular Nucleus of Conscious Rats

Miyake¹,

Share²,

Crofton³

1989

Neuroendocrinology

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We have studied in conscious unrestrained rats the role of angiotensin II receptors in the paraventricular nucleus (PVN) in the control of vasopressin secretion under basal conditions and after 24 h of water deprivation. In euhydrated rats, the microinjection rostral to the PVN of 1 and 5 ng of angiotensin II produced a transient, dose-dependent increase in the plasma vasopressin concentration. There was also a transient, but not dose-dependent, rise in mean arterial blood pressure (MABP), without a change in heart rate. However, the microinjection of angiotensin II into the PVN and sites caudal to it was without effect on the measured variables. In dehydrated rats, on the other hand, microinjection of 5 ng angiotensin II, both into the PVN and rostral to it, resulted in a prolonged increase in the plasma vasopressin concentration. The reponse from the rostral site was more rapid and was associated with an increased MABP. These findings suggest that angiotensin II receptors rostral to the PVN may participate in the control of vasopressin release in both euhydrated and dehydrated states, whereas angiotensin II receptors in the PVN are effective only following dehydration.

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Immunocytochemical Localization of Angiotensin II and Vasopressin in Rat Hypothalamus: Evidence for Production in the Same Neuron

Cited by 105 publications

References 0 publications

Organization of Angiotensin II Immunoreactive Cells and Fibers in the Rat Central Nervous System

Organization of Angiotensin II Immunoreactive Cells and Fibers in the Rat Central Nervous System

Autoradiographic localization of angiotensin II receptors in rat brain.

Effect on Vasopressin Release of Microinjection of Angiotensin II into the Paraventricular Nucleus of Conscious Rats

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