Immunocytochemical localization of Bombyx-PTTH-like molecules in neurosecretory cells of the brain of the migratory locust, Locusta migratoria

Goltzene, Francine; Holder, François; Charlet, Maurice; Meister, Marie; Oka, Tadanori

doi:10.1007/bf00384733

Cited by 35 publications

(15 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The double function of A2 NSN may be achieved through the synthesis of different neurohormones in response to varying environmental conditions. The possibility that one neuron might synthesize different neurohormones has been reported for other insect species (Homberg et al, 1991;Goltzene et al, 1992;Dai et al, 1994). The fact that over 50 neurohormones have been identified, whereas the number of morphologically different NSN in insects is much lower, supports the above hypothesis.…”

Section: Correlation Between Cytological Parameters and The Activity supporting

confidence: 62%

The effect of heat stress on the activity of A1 and A2 neurosecretory neurons of Morimus funereus (Coleoptera: Cerambycidae) larvae

Leković¹,

Lazarević²,

Nenadovic³

et al. 2001

Eur. J. Entomol.

View full text Add to dashboard Cite

Abstract. A study on the effect of heat stress on the activity of the medial A1 and A2 neurosecretory neurons (NSN), and the activity of midgut a-amylase and protease was performed on the fifth larval instar of Morimus funereus. Exposure of the larvae to 35°C led to a decrease in the activity of both A1 and A2 NSN, the former being more marked than the latter. These neurons responded differently to heat stress in terms of neurosecretory material synthesis and release. The changes in the size of both A1 NSN and their nuclei and protease activity in the larvae exposed to elevated temperature were significantly correlated, which sug gests that A1 NSN play a role in the regulation of the activity of this enzyme during heat stress. The significant correlations between the size of A2 NSN and protease activity in the controls, and the size of both A2 NSN and their nuclei and amylase activity in larvae exposed to heat stress, similarly may mean that A2 NSN regulate both digestive enzyme activities depending on environmental tem perature.

show abstract

Section: Correlation Between Cytological Parameters and The Activity supporting

confidence: 62%

The effect of heat stress on the activity of A1 and A2 neurosecretory neurons of Morimus funereus (Coleoptera: Cerambycidae) larvae

Leković¹,

Lazarević²,

Nenadovic³

et al. 2001

Eur. J. Entomol.

View full text Add to dashboard Cite

show abstract

“…The data available on the synthesis of insulin-related molecules in invertebrates suggest the CNS as the major site of biosynthesis : the A2 median neurosecretory cells in the brain of L. migratoria (GoltzenC et al, 1992), the light green cells and the canopy cells in L. stugnulis and four pairs of median neurosecretory cells in B. mori (Mizoguchi et al, 1987). From a phylogenetic point of view, it is interesting to note that these observations are in favour of the hypothesis that the vertebrate pancreatic cells derive from migrating neurodemic cells (Alpert et al, 1988).…”

Section: Discussionmentioning

confidence: 99%

Expression of the gene encoding an insulin‐related peptide inLocusta (Insecta, Orthoptera)

Kromer‐Metzger

Lagueux

1994

European Journal of Biochemistry

View full text Add to dashboard Cite

A peptide with significant sequence similarity to mammalian insulins, Locustu insulin-related peptide (LIRP) has recently been isolated from neurohaemal lobes of Lacustu migrutoriu and the corresponding cDNA has been cloned. We report here the cloning of the LIRP gene and we show that this gene is present as a single copykaploid genome. The organization of this gene is similar to that of mammalian insulin genes. There are at least two LIRP transcripts, LIRP T1 and LIRP T2, differing in their 5' untranslated regions and in the 5' end of the coding region resulting in an additional ATG present in LIRP T2. The two transcripts are differentially expressed in Locustu; whereas LIRP T1 is only expressed in neurosecretory cells of the brain, LIRP T2 is present at low levels in all tissues. Our data indicate that the LIRP gene has at least two promoters, the alternative usage of which could account for a differential regulation of expression of LIRP in the neurosecretory cells and in various peripheral tissues.

show abstract

“…The somata of PTTH-producing cells have been localized in the PL in lepidopteran insects such as B. mori and A. pernyi (Mizoguchi et al 1990;Sauman and Reppert 1996), and in a hemipteran insect, Rhodnius prolixus (Vafopoulou et al 2007). In contrast, PTTH-ir occurs in the PI of the migratory locust, Locusta migratoria (Goltzené et al 1992) as in P. americana, and shows species variability. In situ hybridization has localized three kinds of PKC, viz., dPKC53E(ey) (accession no.…”

Section: Discussionmentioning

confidence: 80%

Putative regulatory mechanism of prothoracicotropic hormone (PTTH) secretion in the American cockroach, Periplaneta americana as inferred from co-localization of Rab8, PTTH, and protein kinase C in neurosecretory cells

2009

View full text Add to dashboard Cite

Small GTPases of the Rab family act as essential regulators of vesicle transport pathways, including the exocytosis of neurohormones. These processes are not well-understood in insects. To address the physiological function of Rab proteins and their phosphorylation in insect neurosecretion, Rab8-like, prothoracicotropic hormone (PTTH)-like, and protein kinase C (PKC)-like immunohistochemical reactivities (-ir) were investigated in the brain of the American cockroach, Periplaneta americana. All the antibodies tested reacted with neurons in the pars intercerebralis, corpora cardiaca, and nervi corporis allati I. Double-labeling experiments demonstrated that all PTTH-ir were colocalized with Rab8-ir and PKC-ir in the pars intercerebralis, although exclusive reactivity was present to antisera against Rab8 or PKC. These findings support the notion that Rab8-like antigen is phosphorylated by PKC, and that this phosphorylation is involved in the axonal transport and secretion of PTTH in this species.

show abstract

Immunocytochemical localization of Bombyx-PTTH-like molecules in neurosecretory cells of the brain of the migratory locust, Locusta migratoria

Cited by 35 publications

References 30 publications

The effect of heat stress on the activity of A1 and A2 neurosecretory neurons of Morimus funereus (Coleoptera: Cerambycidae) larvae

The effect of heat stress on the activity of A1 and A2 neurosecretory neurons of Morimus funereus (Coleoptera: Cerambycidae) larvae

Expression of the gene encoding an insulin‐related peptide inLocusta (Insecta, Orthoptera)

Putative regulatory mechanism of prothoracicotropic hormone (PTTH) secretion in the American cockroach, Periplaneta americana as inferred from co-localization of Rab8, PTTH, and protein kinase C in neurosecretory cells

Contact Info

Product

Resources

About