Immunocytochemical localization of calretinin in the forebrain of the rat

Abstract

The distribution of the calcium binding protein calretinin (protein 10) was examined in the rat forebrain by immunohistochemistry. The main and accessory olfactory bulbs had immunoreactive label in granule, periglomerular, and mitral cells. Positive fibers were noted in the external plexiform and granule cell layers, glomeruli, and in the molecular layer of the anterior olfactory nucleus. The cerebral cortex contained calretinin label in nonpyramidal bipolar cells. Cells in the substantia nigra compacta and ventral tegmental area were also calretinin positive as were nigrostriatal and mesolimbic projections (caudate-putamen, nucleus accumbens). In the hippocampus, interneurons were stained in all the subfields of the CA1-CA4 regions. In the thalamus, many positive cells were observed in the periventricular, reticular, lateral habenula, and reunions nuclei. Calretinin immunoreactive cells were particularly abundant in the lateral mamillary and septofimbrial nuclei. Several fiber tracts were also revealed, i.e., the lateral olfactory tract, mamillothalamic tract, fasciculus retroflexus, optic tract, and stria medullaris. These results demonstrate a distinct distribution of calretinin within cell bodies and fibers.

Vesicular glutamate transporter 3-immunoreactive pericellular baskets ensheath a distinct population of neurons in the lateral septum

“…Secondly, we wanted to reveal co-localization and the spatial relationship of the VGLUT3-ir PBs with well-defined neuron subpopulations and/or the fiber systems projecting to and passing the LS. Thus, we performed single and double immunofluorescence staining of VGLUT3 and the calcium-binding proteins calbindin (CALB), calretinin (CALR) and parvalbumin (PARV), with choline acetyltransferase (ChAT), tyrosine hydroxylase (TH) and tryptophan hydroxylase (TrpH), and with Wisteria floribunda agglutinin (WFA) revealing perineuronal nets (Bialowas and Frotscher, 1987;Gall and Moore, 1984;Jacobowitz and Winsky, 1991;Kiss et al, 1997;Seeger et al, 1994;Seifert et al, 1998). Triple labeling of VGLUT3 with VGLUT1 and VGLUT2 was performed, as the LS is known to be positive for both glutamatergic markers and it was recently suggested, that projection neurons in the intermediate and ventral LS use glutamate as neurotransmitter (Kaneko et al, 2002;Kocsis et al, 2003;Lin et al, 2003).…”

Calcium-Binding Proteins in the Central Nervous System.

“…A, C X 19, B, DX23 possible disadvantage in using the antibodies for these structurally related proteins (49). Maps of immunohistochemical localization of PV, CB and CR in the rat brain have been reported (4,14,46,84). Immunopositive cell populations are mostly similar among the various species examined but species differences in distribution such as the presence of PV in the thalamic relay fibers in the monkey but not in the rat (48) have been reported, which indicates that these proteins are not "freely granted" to a given cell type (see 1) but some factors decide their expression.…”

Prolonged Glial Expression of Sox4 in the CNS Leads to Architectural Cerebellar Defects and Ataxia

“…Although minor aberrations and an overall thinning of the cerebral cortex were observed at P9, the principal layering was indeed present. This was evident from histological as well as immunohistochemical stainings for both calretininpositive nonpyramidal neurons in layers 1-3 (Jacobowitz and Winsky, 1991) and Oct6-positive neurons in layers 2/3 and 5 (Bermingham et al, 1996) (Fig. 8 D-F ).…”