1985
DOI: 10.1267/ahc.18.597
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Immunocytochemical localization of catecholestrogen in the rat liver after using various fixatives.

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Cited by 4 publications
(2 citation statements)
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“…After sections were washed with 0.01 mol/ L phosphate-buffered saline (PBS), they were subsequently incubated with a 1:50 dilution (in PBS) of normal goat serum for 30 min at room temperature. Sections were incubated for 24 hours at 40C in a 1:2000 dilution (in PBS) of either rabbit antiserum to 2-hydroxy-estrone-17-(O-carboxymethyl)-oxime bovine serum albumin (BSA) conjugate (Inoue and Yoshizawa, 1985), or rabbit antiserum to rat liver COMT (Grossman et al, 1985), or, as a control, in normal rabbit serum; then in a 1:50 dilution (in PBS) of goat antirabbit IgG (Cappel Laboratories, Downingtown, PA) for 30 minutes; and finally in a 1:100 (in PBS) dilution of peroxidase-antiperoxidase complex (PAP) (Cappel Laboratories) for 30 min at room temperature. All slides were washed for 15 min each in three changes of PBS between each step.…”
Section: Methodsmentioning
confidence: 99%
See 1 more Smart Citation
“…After sections were washed with 0.01 mol/ L phosphate-buffered saline (PBS), they were subsequently incubated with a 1:50 dilution (in PBS) of normal goat serum for 30 min at room temperature. Sections were incubated for 24 hours at 40C in a 1:2000 dilution (in PBS) of either rabbit antiserum to 2-hydroxy-estrone-17-(O-carboxymethyl)-oxime bovine serum albumin (BSA) conjugate (Inoue and Yoshizawa, 1985), or rabbit antiserum to rat liver COMT (Grossman et al, 1985), or, as a control, in normal rabbit serum; then in a 1:50 dilution (in PBS) of goat antirabbit IgG (Cappel Laboratories, Downingtown, PA) for 30 minutes; and finally in a 1:100 (in PBS) dilution of peroxidase-antiperoxidase complex (PAP) (Cappel Laboratories) for 30 min at room temperature. All slides were washed for 15 min each in three changes of PBS between each step.…”
Section: Methodsmentioning
confidence: 99%
“…The coexistence of catecholestrogens and COMT in the same cell type would strongly suggest a functional relationship between these two factors. The availability of specific antisera for COMT (Grossman et al, 1985) and for catecholestrogens (Inoue and Yoshizawa, 1985) allowed us to examine this possibility in the parotid gland of the rat.…”
Section: Introductionmentioning
confidence: 99%