Immunocytochemical localization of hypocalcin in the endocrine cells of the corpuscles of Stannius in three teleost species (trout, flounder and goldfish)

Bonga, S.E. Wendelaar; Smits, Paul; Flik, G.; Kaneko, Toyoji; Pang, Peter K.T.

doi:10.1007/bf00218804

Cited by 13 publications

(9 citation statements)

References 29 publications

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“…5 It has been demonstrated that CS extracts inhibit Ca 2ϩ uptake by three salmonids and the American eel (380), and stanniectomy of the eel was associated with increased plasma Ca 2ϩ levels, which were corrected when STC was injected (280), so STC is generally considered to be the major hypocalcemic factor in fishes (767). The cellular site of STC production in the CS has been localized by immunocytochemistry (798), and hypercalcemia in the rainbow trout is associated with increased release of immunoreactive STC from the CS (219). Moreover, intraperitoneal injection of CaCl 2 into the rainbow trout and European eel stimulated STC release from the CS (279), as did acclimation of the eel to seawater versus fresh water (278).…”

Section: Stanniocalcinmentioning

confidence: 99%

The Multifunctional Fish Gill: Dominant Site of Gas Exchange, Osmoregulation, Acid-Base Regulation, and Excretion of Nitrogenous Waste

Evans¹,

Piermarini²,

Choe³

2005

Physiological Reviews

2,320

1,788

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The fish gill is a multipurpose organ that, in addition to providing for aquatic gas exchange, plays dominant roles in osmotic and ionic regulation, acid-base regulation, and excretion of nitrogenous wastes. Thus, despite the fact that all fish groups have functional kidneys, the gill epithelium is the site of many processes that are mediated by renal epithelia in terrestrial vertebrates. Indeed, many of the pathways that mediate these processes in mammalian renal epithelial are expressed in the gill, and many of the extrinsic and intrinsic modulators of these processes are also found in fish endocrine tissues and the gill itself. The basic patterns of gill physiology were outlined over a half century ago, but modern immunological and molecular techniques are bringing new insights into this complicated system. Nevertheless, substantial questions about the evolution of these mechanisms and control remain.

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Section: Stanniocalcinmentioning

confidence: 99%

The Multifunctional Fish Gill: Dominant Site of Gas Exchange, Osmoregulation, Acid-Base Regulation, and Excretion of Nitrogenous Waste

Evans¹,

Piermarini²,

Choe³

2005

Physiological Reviews

2,320

1,788

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“…1988: Wagner et al 1988 and the trout (Kaneko el al. 1988;Wendelaar Bonga et al 1989) STC antisera have been characterized previously.…”

Section: Primary Antiseramentioning

confidence: 99%

Immunoreactivity of the corpuscles of Stannius of the garpike, Lepisosteus osseus , to antisera against salmon and trout stanniocalcins

Marra¹,

Youson²,

Bonga³

et al. 1994

Cell and Tissue Research

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Abstract. Stanniocalcin-immunoreactive cells were local ized in the corpuscles of Stannius of a holostean fish, the garpike (Lepisosteus osseus), using antisera against salmon and trout stanniocalcins and the peroxidase-antiperoxidase and protein A-gold immunohistochemical methods. The stanniocalcin-immunoreactive cells were periodic acid-Schiff-positive, and antibody staining was abolished if the antiserum was preabsorbed with corpus cle homogenate. Immunocytochemistry revealed two re active cell types in the glandular parenchyma, and im munoreactivity was confined to the secretory granules. Staining of the granules was also abolished when the antisera were blocked with crude corpuscle homogenate. When corpuscle extracts from garpike were subjected to sodium dodecyl sulphate-polyacrylamide gel elec trophoresis and Western blot analysis, a single dense band was evident with a molecular weight of ^68 kDa under non-reducing conditions, whereas three bands were observed (^29, -31, and ^3 4 kDa) under reduc ing conditions. Staining of all bands disappeared follow ing preabsorption of the antiserum with salmon stanniocalcin, trout stanniocalcin, or garpike corpuscle extract. The results are compared with stanniocalcins from an other extant holostean, the bowfin (Amia calva), and from more modern bony fishes, the teleosts.

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“…Polyclonal rabbit anti-STC1 antibodies were a gift from Dr. Gert Flik (Radboud University, Nijmegen, Netherlands). 35 Antibodies for mouse pan-macrophage marker (CD68) were purchased from AbD Serotec (Raleigh, NC). Actin antibodies were purchased from Chemicon (Temecula, CA).…”

Section: Methodsmentioning

confidence: 99%

“…Five-m sections were cut, dried, and rehydrated. Labeling was performed using polyclonal rabbit anti-STC1 (1:1000 dilution) 35 or monoclonal rat anti-mouse F4/80 antigen antibodies (1:100 dilution), and detection was performed using peroxidase enzyme-based detection system (Vector Laboratories, Burlingame, CA). Control for labeling was performed in the presence of nonimmune IgG, and showed no staining.…”

Section: Immunohistochemistrymentioning

confidence: 99%

Anti-Inflammatory and Renal Protective Actions of Stanniocalcin-1 in a Model of Anti-Glomerular Basement Membrane Glomerulonephritis

Huang

García

Lou

et al. 2009

The American Journal of Pathology

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We have previously shown that stanniocalcin-1 (STC1) inhibits the transendothelial migration of macrophages and T cells, suppresses superoxide generation in macrophages, and attenuates macrophage responses to chemoattractants. To study the effects of STC1 on inflammation, in this study we induced a macrophage-and T-cell-mediated model of anti-glomerular basement membrane disease in STC1 transgenic mice, which display elevated serum STC1 levels and preferentially express STC1 in both endothelial cells and macrophages. We examined the following parameters both at baseline and after anti-glomerular basement membrane antibody treatment: blood pressure; C 3a levels; urine output; proteinuria; blood urea nitrogen; and kidney C 3 deposition, fibrosis, histological changes, cytokine expression, and number of T cells and macrophages. Compared with wild-type mice, after anti-glomerular basement membrane treatment STC1 transgenic mice exhibited: i) diminished infiltration of inflammatory macrophages in the glomeruli; ii) marked reduction in crescent formation and sclerotic glomeruli; iii) decreased interstitial fibrosis; iv) preservation of kidney function and lower blood pressure; v) diminished C 3 deposition in the glomeruli; and vi) reduced expression of macrophage inhibitory protein-2 and transforming growth factor-␤2 in the kidney. Compared with baseline , wild-type mice , but not STC1 transgenic mice , had higher proteinuria and a marked reduction in urine output. STC1 had minimal effects , however , on both T-cell number in the glomeruli and interstitium and on cytokine expression characteristic of either TH1 or TH2 activation. These data suggest that STC1 is a potent anti-inflammatory and renal protective protein. Stanniocalcin-1 (STC1) is a 25-kDa homodimeric glycoprotein hormone involved in calcium regulation in bony fish, 1 in which elevation of serum calcium triggers the release of STC1 from the corpuscles of Stannius, 2 organs associated with the kidneys.3 On circulation in the gill and intestine, STC1 inhibits calcium influx from the aquatic environment to the blood to maintain stable concentrations of calcium in the blood. 4 Mammalian STC1 mRNA is ubiquitously expressed, and the highest levels of STC1 expression are found in the ovary, kidney, prostate, and thyroid. [5][6][7] It was previously suggested that STC1 protein does not circulate in the blood of mammals 8 except during pregnancy and lactation 9 ; however, recent data suggest that mammalian STC1 is blood-borne , attached to a soluble protein. 10 The cellular distribution of STC1 mRNA and protein in mammalian organs is not always parallel. In the kidney for example, in situ hybridization revealed restricted expression of STC1 mRNA in the cortical and medullary collecting ducts, whereas the protein is detected along the entire nephron. 11,12 Similarly, the distribution of STC1 mRNA does not parallel the distribution of the protein in cellular elements of the ovary and pregnant uterus.

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Immunocytochemical localization of hypocalcin in the endocrine cells of the corpuscles of Stannius in three teleost species (trout, flounder and goldfish)

Cited by 13 publications

References 29 publications

The Multifunctional Fish Gill: Dominant Site of Gas Exchange, Osmoregulation, Acid-Base Regulation, and Excretion of Nitrogenous Waste

The Multifunctional Fish Gill: Dominant Site of Gas Exchange, Osmoregulation, Acid-Base Regulation, and Excretion of Nitrogenous Waste

Immunoreactivity of the corpuscles of Stannius of the garpike, Lepisosteus osseus , to antisera against salmon and trout stanniocalcins

Anti-Inflammatory and Renal Protective Actions of Stanniocalcin-1 in a Model of Anti-Glomerular Basement Membrane Glomerulonephritis

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