Immunocytochemical localization of lysozyme and β-defensin in the apocrine glands of the equine scrotum

Yasui, Tadashi; Fukui, Kousuke; Nara, Takayuki; Habata, Isao; Meyer, Wilfried; Tsukise, Azuma

doi:10.1007/s00403-007-0766-5

Cited by 18 publications

(18 citation statements)

References 30 publications

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“…These findings corroborate the view that the fine and dense blood vessel network of the scrotal dermis is generally involved in thermoregulation by heat emission [5,7,13]. In this context it has to be emphasized that the secretions of the apocrine tubular glands are not involved in thermoregulative activities but in antimicrobial defence to protect the skin and, here particularly the sparsely haired scrotal surface, against microbial invasion [15,18,20,21,24,25]. In this context, the excellent vascular supply of the scrotal apocrine glands, as shown in our study, supports strong and continuous secretion production.…”

Section: A B Bsupporting

confidence: 82%

Microvascularisation of the scrotal integument of Holstein-Friesian bulls

et al. 2013

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Section: A B Bsupporting

confidence: 82%

Microvascularisation of the scrotal integument of Holstein-Friesian bulls

et al. 2013

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“…All substances in question are transported onto the epidermal surface as their normal biotope for effective extracorporal use (e.g., Meyer et al 2003b;Meyer and Seegers 2004;Meyer 2007). In general, the immune system had to develop a special integumental subsystem, and the mammalian skin can be regarded as a tertiary immune organ, whereby the keratinocyte, in particular, is another major source for different innate immunityrelated humoral compounds, such as defensins, complement factors, and complement regulatory proteins (e.g., Harder Debenedictis et al 2001;Yang et al 2001;Meyer and Seegers 2004;Yasui et al 2007).…”

Section: Introductionmentioning

confidence: 99%

Demonstration of β-glucan receptors in the skin of aquatic mammals—a preliminary report

Meyer

Kloepper²,

Fleischer

2008

Eur J Wildl Res

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Using immunohistochemistry, the study clearly demonstrates three important β-glucan receptors (Ficolin/ P35, MBL, Dectin-1; members of the lectin-complement pathway of innate immunity) in the integument of six marine and freshwater aquatic mammals (Northern fur seal, Common seal, Walrus, Coypu, Capybara, Otter), but only weakly in two dolphin species. Most of the non-dolphin mammals exhibited strong reactions, especially with regard to the skin glands (tubular apocrine glands, sebaceous glands), for L-Ficolin/P35 and MBL. Distinct reaction staining could also be observed in the epidermis and the outer epithelial sheath of primary hair follicles.Positive Dectin-1 staining was limited to secretory cells of the apocrine tubular glands, and to peripheral and central cells of sebaceous glands of the seals. The Capybara was the only animal to show a clear Dectin reaction in the epidermis (stratum granulosum).The findings are discussed with regard to the constant and high microbial challenge of the skin in the aquatic medium, and variations in hair density of the animals.

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“…For the detection of b-defensin protein, an anti human b-defensin-2 antibody was used that had been shown previously to cross-react with equine tissue (Yasui et al 2007). This antibody was applied, since equine b-defensin-1 displays the greatest peptide sequence homologies to human b-defensin-2 and porcine b-defensin-1 (Davis et al 2004, Looft et al 2006 and no commercial antibody for labelling of equine b-defensin-1 was available at the time of this study.…”

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confidence: 99%

Beta-defensin is a component of the endometrial immune defence in the mare

Schöniger¹,

Grafe²,

Schoon³

2013

PHK

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SummaryDefensins are antimicrobial peptides of the innate immune defence. The aim of the present study was to examine if b-defensin is expressed in the equine endometrium. Endometrial tissue samples were available from 29 mares. Performed were a histological examination (n=28), and immunohistochemistry (n =28). Mares aged 3 -20 years. Endometrosis was diagnosed in 20 mares; in the biopsy of 1 mare, endometrial glands could not be evaluated. Endometritis was observed in 12 and angiosclerosis in 21 mares. No endometrial alterations were found in 3 mares. Expression of equine b-defensin-1 mRNA was detected in all 29 mares and confirmed by sequencing in 11 cases. Immunostaining for b-defensin was detected in all 28 mares investigated; however, in 1 mare the surface and in another mare the glandular epithelium could not be evaluated. Immunopositive cell populations were the luminal epithelium (n=27), the glandular epithelium (n=20), stromal cells (n=6) and the vascular tunica media (n=14). The majority of immunopositive cells were located within the luminal epithelium and the duct of the endometrial glands. The immunostainig of the epithelium was predominantly cytoplasmic and less frequently nuclear. Stromal cells showed a nuclear staining. The smooth muscle cells of the vascular tunica media displayed a cytoplasmic labelling in 13 mares and a nuclear staining in 1 mare. This study demonstrates the expression of b-defensins in the healthy and diseased equine endometrium and indicates that b-defensin contributes to the endometrial immune defence in the mare.

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Immunocytochemical localization of lysozyme and β-defensin in the apocrine glands of the equine scrotum

Cited by 18 publications

References 30 publications

Microvascularisation of the scrotal integument of Holstein-Friesian bulls

Microvascularisation of the scrotal integument of Holstein-Friesian bulls

Demonstration of β-glucan receptors in the skin of aquatic mammals—a preliminary report

Beta-defensin is a component of the endometrial immune defence in the mare

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