Immunocytochemical localization of rhodopsin-like immunoreactivity in the outer segments of the rods and single cones of chick retina.

Araki, Masasuke; Watanabe, Kenji; Yasuda, Kazuaki

doi:10.1247/csf.9.1

Cited by 20 publications

(4 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…We show that the principal cone lacks an axon, in agreement with the shapes drawn by others (Gallego 1976 ; Gayoso et al 1978 ; Morris and Shorey 1967 ; Araki et al 1984 ) but in contrast to the description of the principal cone by Ramón y Cajal ( 1892 ). In all the cone couples studied, only the accessory cones are seen to have an axon and, furthermore, the percentage of principal and accessory cones found (26.97% each) is similar to that found by Oishi et al ( 1990 ), viz.…”

Section: Discussionsupporting

confidence: 92%

“…Since the studies of Ramón y Cajal ( 1892 ), the oblique cone has been practically ignored. Indeed, with a few exceptions (Gallego 1976 ; Gallego et al 1975 ; Gayoso et al 1978 ; Mariani and Leure-duPree 1978 ), the studies of avian cones and particularly those of the chicken (Araki et al 1984 ; Bowmaker et al 1997 ; Bowmaker and Knowles 1977 ; Meyer and May 1973 ; Morris 1970 ; Morris and Shorey 1967 ; Oishi et al 1990 ; Szel et al 1985 ) refer to single cones and double cones, without distinguishing straight from oblique cones in the pool of single cones.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Isolation of chick retina cones and study of their diversity based on oil droplet colour and nucleus position

et al. 2008

View full text Add to dashboard Cite

The chick retina has four morphological cone types that differ not only in shape, but also in the visual pigment in the outer segment, in the colour of the oil droplet in the inner segment and in synaptic connectivity. Neither the type of droplet nor the visual pigment has been definitively established for the four cone types. The main aim of the present work has been the isolation of entire live photoreceptors in order to study the oil droplet colour in each cone type and to quantify each type. We have improved an earlier retinal cell isolation method and obtained large numbers of entire cones. Principal cones (27% of the cones) possess a yellow or colourless droplet. Accessory cones (27% of the cones) all contain a small pale green droplet. Straight cones (44% of the cones) have a red, orange, yellow, or colourless droplet. Oblique cones (1.66% of the cones) all have a colourless droplet. We have found that straight cones with a red, orange, or yellow droplet differ in terms of the position of the nucleus and their percentage and conclude that they are distributed in three rows in the outer nuclear layer (ONL) of the central retina. Our study of 4,6-diamidino-2-phenylindole-stained retinal sections has revealed three rows of nuclei instead of the two currently thought to form the ONL. Together, our results show a larger cone diversity than previously known, suggest a larger functional diversity and provide an efficient method for isolating entire chick photoreceptors.

show abstract

Section: Discussionsupporting

confidence: 92%

Section: Introductionmentioning

confidence: 99%

Isolation of chick retina cones and study of their diversity based on oil droplet colour and nucleus position

et al. 2008

View full text Add to dashboard Cite

show abstract

“…red/green opsin ( Fig. 3e-g) but not for rhodopsin (data not shown; Araki et al, 1984). Because the calbindin-IR photoreceptors terminate only in the outer stratum of the OPL, these data show that calbindin is a specific marker for principal and accessory members of double cones in the chicken retina.…”

Section: Connections Of Trka-and Calretinin-ir Horizontal Cells With mentioning

confidence: 66%

Heterogeneity of horizontal cells in the chicken retina

Fischer

Stanke

Aloisio

et al. 2006

J of Comparative Neurology

View full text Add to dashboard Cite

Despite numerous reports that different markers are expressed by horizontal cells in the avian retina, it remains unknown whether different types of horizontal cells can be defined by differences in their immunocytochemical profiles. The purpose of this study was to rectify this deficiency. We identified horizontal cells by indirect immunofluorescence with antibodies to calretinin, trkA, GABA, Prox1, AP2alpha, Pax6, islet1, and Lim1 + 2. We found two major groups of horizontal cells, those that express trkA and those that express calretinin. The trkA-immunoreactive (-IR) horizontal cells had small, round somata and robust, bulbous dendritic endings, whereas calretinin-IR horizontal cells had large, polygonal cell bodies and fine, diffuse dendritic endings, both contacting the calbindin-IR pedicles of double cones. Weak gamma-aminobutyric acid (GABA) immunoreactivity was observed only in a few of the trkA-IR horizontal cells, whereas the overlap of calretinin and GABA immunoreactivities was 100%. The majority of trkA-IR horizontal cells expressed islet1, and the majority of calretinin-IR horizontal cells expressed Lim1 + 2, AP2alpha, and Pax6. Islet1 immunoreactivity was observed in a small fraction of calretinin-IR/non-trkA-IR cells. In agreement with previous reports, we detected Prox1 immunoreactivity in all types of horizontal cells. These immunolabeling profiles suggest that there are four immunochemically distinct subtypes of horizontal cells in the postnatal chick retina, which may match the four types that have been observed in Golgi-impregnated pigeon and turtle retinas.

show abstract

“…In the development of the chick retina, both Rho-LI and Iodo-LI cells are found first at E14 (Araki et al 1984(Araki et al , 1990 and in the quail retina at E13 (unpublished observations). Thus, the synthesis of rhodopsin and iodopsin seems to begin at about the same stage in the pineal organ and the retina of develop-ing avian embryos.…”

Section: Resultsmentioning

confidence: 97%

Differentiation of pinopsin-immunoreactive cells in the developing quail pineal organ: an in-vivo and in-vitro immunohistochemical study

Yamao

Araki

Okano

et al. 1999

Cell and Tissue Research

Self Cite

View full text Add to dashboard Cite

The avian pineal organ contains several types of photoreceptors with different photopigments: rhodopsin, iodopsin, and pinopsin. We have previously examined the differentiation of both rhodopsin-like and iodopsin-like immunoreactive cells during pineal development in quail embryos to determine the onset of synthesis of specific proteins and their cellular localization. In the present study, we have performed pinopsin immunohistochemistry on in-vivo developing and in-vitro cultured pineal organs of quail embryos. The results were compared with those obtained with rhodopsin and iodopsin immunohistochemistry. In the developing pineal organs, pinopsin immunoreactivity was detected at embryonic day 8, i.e. five days earlier than rhodopsin-like and iodopsin-like immunoreactivities. It was localized exclusively in the protrusions extending into the lumen throughout development, whereas rhodopsin-like and iodopsin-like immunoreactivities were usually found both in cell bodies and processes. These differences were also observed under two different types of culture conditions (dissociated cell culture and organ culture) indicating that, in the avian pineal organ, the expression pattern of the pinopsin gene is basically different from those of the other two pineal photopigments. The present study suggests that pineal cells have a mechanism for the polarized transport of pinopsin molecules.

show abstract

Immunocytochemical localization of rhodopsin-like immunoreactivity in the outer segments of the rods and single cones of chick retina.

Cited by 20 publications

References 22 publications

Isolation of chick retina cones and study of their diversity based on oil droplet colour and nucleus position

Isolation of chick retina cones and study of their diversity based on oil droplet colour and nucleus position

Heterogeneity of horizontal cells in the chicken retina

Differentiation of pinopsin-immunoreactive cells in the developing quail pineal organ: an in-vivo and in-vitro immunohistochemical study

Contact Info

Product

Resources

About