Immunocytochemical mapping of a C-terminus anti-peptide antibody to the GABA receptor subunit, RDL in the nervous system of Drosophila melanogaster

Harrison, J.; Chen, H. H.; Sattelle, E.; Barker, Paul D.; Huskisson, Neville S.; Rauh, James J.; Bai, Donglin; Sattelle, David B.

doi:10.1007/s004410050587

Cited by 74 publications

(77 citation statements)

References 38 publications

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“…In the adult fly, we observed widespread, punctate labeling for dVGAT throughout the adult central brain and optic lobes (Fig.5), consistent with previous reports using GABAergic markers in Drosophila and other insects (Brotz and Borst, 1996;Brotz et al, 2001;Enell et al, 2007;Harrison et al, 1996;Meyer et al, 1986;Sinakevitch et al, 2003;Sinakevitch and Strausfeld, 2004;Strausfeld et al, 1995). Labeled structures in the central brain include the subesophageal ganglion (Fig.5A,B, SOG), antennal lobe (Fig.5A, Al) and both the ellipsoid body ( Fig.…”

Section: Dvgat Is Widely Expressed In the Adult Nervous Systemsupporting

confidence: 90%

Mutation of theDrosophilavesicular GABA transporter disrupts visual figure detection

Fei

Chow

Chen

et al. 2010

Journal of Experimental Biology

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SUMMARYThe role of gamma amino butyric acid (GABA) release and inhibitory neurotransmission in regulating most behaviors remains unclear. The vesicular GABA transporter (VGAT) is required for the storage of GABA in synaptic vesicles and provides a potentially useful probe for inhibitory circuits. However, specific pharmacologic agents for VGAT are not available, and VGAT knockout mice are embryonically lethal, thus precluding behavioral studies. We have identified the Drosophila ortholog of the vesicular GABA transporter gene (which we refer to as dVGAT), immunocytologically mapped dVGAT protein expression in the larva and adult and characterized a dVGAT minos mutant allele. dVGAT is embryonically lethal and we do not detect residual dVGAT expression, suggesting that it is either a strong hypomorph or a null. To investigate the function of VGAT and GABA signaling in adult visual flight behavior, we have selectively rescued the dVGAT mutant during development. We show that reduced GABA release does not compromise the active optomotor control of wide-field pattern motion. Conversely, reduced dVGAT expression disrupts normal object tracking and figure-ground discrimination. These results demonstrate that visual behaviors are segregated by the level of GABA signaling in flies, and more generally establish dVGAT as a model to study the contribution of GABA release to other complex behaviors.

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Section: Dvgat Is Widely Expressed In the Adult Nervous Systemsupporting

confidence: 90%

Mutation of theDrosophilavesicular GABA transporter disrupts visual figure detection

Fei

Chow

Chen

et al. 2010

Journal of Experimental Biology

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“…Similar to the GABA receptors in the general population of neurons, these are PTX-sensitive and are not blocked by BMC. Based on localization studies (Harrison et al, 1996) and their pharmacological profile, it is tempting to speculate that these synaptic receptors contain Rdl subunits, similar to the PTX-sensitive synaptic GABA receptors described previously in embryonic cultured neurons (Lee et al, 2003). However, there are some significant differences in the GABAergic synaptic currents in the pupal and embryonic neurons.…”

Section: Discussionmentioning

confidence: 58%

Fast Synaptic Currents inDrosophilaMushroom Body Kenyon Cells Are Mediated by α-Bungarotoxin-Sensitive Nicotinic Acetylcholine Receptors and Picrotoxin-Sensitive GABA Receptors

2003

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“…GABA-immunoreactive LNs are present in the adult antennal lobe of several species (Hoskins et al, 1986;Malun, 1991;Leitch and Laurent, 1996;Bicker, 1999) and in the larval Drosophila antennal lobe (Python and Stocker, 2002). Antennal lobe LNs can synaptically inhibit PNs (Christensen et al, 1993;MacLeod and Laurent, 1996), and the antennal lobe is strongly immunoreactive for GABA A receptors (Harrison et al, 1996). However, GABA A antagonists do not block odor-evoked slow inhibition or slow temporal patterns in PNs (MacLeod and Christensen et al, 1998;Wilson et al, 2004).…”

Section: Introductionmentioning

confidence: 99%

Role of GABAergic Inhibition in Shaping Odor-Evoked Spatiotemporal Patterns in theDrosophilaAntennal Lobe

Wilson¹,

Laurent²

2005

J. Neurosci.

440

534

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Drosophila olfactory receptor neurons project to the antennal lobe, the insect analog of the mammalian olfactory bulb. GABAergic synaptic inhibition is thought to play a critical role in olfactory processing in the antennal lobe and olfactory bulb. However, the properties of GABAergic neurons and the cellular effects of GABA have not been described in Drosophila, an important model organism for olfaction research. We have used whole-cell patch-clamp recording, pharmacology, immunohistochemistry, and genetic markers to investigate how GABAergic inhibition affects olfactory processing in the Drosophila antennal lobe. We show that many axonless local neurons (LNs) in the adult antennal lobe are GABAergic. GABA hyperpolarizes antennal lobe projection neurons (PNs) via two distinct conductances, blocked by a GABA A -and GABA B -type antagonist, respectively. Whereas GABA A receptors shape PN odor responses during the early phase of odor responses, GABA B receptors mediate odor-evoked inhibition on longer time scales. The patterns of odor-evoked GABA B -mediated inhibition differ across glomeruli and across odors. Finally, we show that LNs display broad but diverse morphologies and odor preferences, suggesting a cellular basis for odor-and glomerulus-dependent patterns of inhibition. Together, these results are consistent with a model in which odors elicit stimulus-specific spatial patterns of GABA release, and as a result, GABAergic inhibition increases the degree of difference between the neural representations of different odors.

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Immunocytochemical mapping of a C-terminus anti-peptide antibody to the GABA receptor subunit, RDL in the nervous system of Drosophila melanogaster

Cited by 74 publications

References 38 publications

Mutation of theDrosophilavesicular GABA transporter disrupts visual figure detection

Mutation of theDrosophilavesicular GABA transporter disrupts visual figure detection

Fast Synaptic Currents inDrosophilaMushroom Body Kenyon Cells Are Mediated by α-Bungarotoxin-Sensitive Nicotinic Acetylcholine Receptors and Picrotoxin-Sensitive GABA Receptors

Role of GABAergic Inhibition in Shaping Odor-Evoked Spatiotemporal Patterns in theDrosophilaAntennal Lobe

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