Immunocytochemical studies of relaxin in ovaries of pregnant and cycling mice.

Vaupel, Matthias; Sherwood, O. D.; Anderson, Mary B.

doi:10.1177/33.4.2579996

Cited by 12 publications

(10 citation statements)

References 11 publications

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“…Our results are in agreement with those of previous studies using a peroxidase-antiperoxidase method, in which immunostaining for relaxin was positive at D 10 [3] or at D M [4], Anderson et al [3] also reported relaxin localization in the peripheral cells of the CL on Dio, spreading out with the progression of pregnancy. In our study, the immunostaining was always distributed throughout the whole CL.…”

Section: Discussionsupporting

confidence: 83%

“…Relaxin was observed only in type I CL. This is in agreement with the results of Vaupel et al [4] who found that only the CL originated by the onset of pregnancy are stained.…”

Section: Discussionsupporting

confidence: 83%

“…Relaxin has been detected by bioassay in the ovaries of pregnant mice [1] and by radioimmunoassay in the serum during the last half of pregnancy [2], Other stud ies, using the peroxidase-antiperoxidase immunocyto chemical method with an antirat relaxin antiserum, have established that, in mice, relaxin was present in the cor pora lutea (CL) during the second part of gestation [3,4] and only in CL which originated at the onset of preg nancy [4], Although immunocytochemical methods provide mostly qualitative information, they may also be used to quantify antigens in a tissue. Indeed, under well-estab lished conditions, the intensity of labeling can be related to the amount of antigen [5],…”

Section: Introductionmentioning

confidence: 99%

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Changes of Relaxin Concentrations Determined by Immunodensitometry in Ovaries of NMRI Mice during Pregnancy

Psalti

Rahier²,

Loumaye

et al. 1990

Gynecol Obstet Invest

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Relaxin has been localized in corpora lutea (CL) of pregnant NMRI mice using the avidin-biotin complex immunocytochemical procedure and an antiserum against highly purified porcine relaxin. The immunostaining was measured by immunodensitometry. Relaxin immunostaining was first observed in luteal cells of type I gestational CL on day 11.5 (D_11.5), For each investigated day, all CL were identically stained, and immunostaining was evenly dispersed all over the CL. Seventy-five percent of cells were stained at D_11.5, and nearly all cells were stained betweeen D_13.5 and D_18.5. The staining intensity increased throughout the last half of pregnancy, reaching a maximum at Dig. A few hours before parturition, at D_18.5, relaxin immunostaining decreased dramatically and reached the background level shortly after delivery. From our results we may conclude that, in murine CL, the number of relaxin-secreting cells and the intracellular storage of the peptide increase during pregnancy. The disappearance of relaxin with the cells occurs rapidly ± 12 h before parturition.

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Section: Discussionsupporting

confidence: 83%

“…Relaxin was observed only in type I CL. This is in agreement with the results of Vaupel et al [4] who found that only the CL originated by the onset of pregnancy are stained.…”

Section: Discussionsupporting

confidence: 83%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Changes of Relaxin Concentrations Determined by Immunodensitometry in Ovaries of NMRI Mice during Pregnancy

Psalti

Rahier²,

Loumaye

et al. 1990

Gynecol Obstet Invest

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“…For example, iloprost, a synthetic prostacyclin analog (48), has been reported to improve skin tightness in scleroderma patients (49,50), probably because of its ability to reduce the expression of type I collagen and connective tissue growth factor in fibroblasts (50). In addition, relaxin, a hormone produced by the corpus luteum in high concentrations during pregnancy (51,52), was found to significantly reduce collagen synthesis in fibroblasts; this effect could be amplified by the addition of interferon-␥, a cytokine that down-regulates the expression of collagen genes (53). Interestingly, relaxin may also influence the development of the T cellmediated immune response, skewing it toward a Th1 type of response (54).…”

Section: Discussionmentioning

confidence: 99%

Halofuginone inhibition of COL1A2 promoter activity via a c‐Jun–dependent mechanism

McGaha¹,

Kodera²,

Spiera³

et al. 2002

Arthritis & Rheumatism

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Objective. The naturally occurring compound halofuginone has been shown to antagonize collagen synthesis by fibroblasts both in vitro and in vivo. We previously demonstrated that this inhibitory property was related to the ability of halofuginone to disrupt transforming growth factor ␤ signal transduction. The present study further analyzed the ability of halofuginone to affect transcription factors that can regulate type I collagen gene expression by examining its effect on c-Jun, the negative regulator of collagen gene transcription.Methods. The phosphorylation state of c-Jun in the presence of halofuginone was examined via direct Western blotting, and the transcriptional activity of the activator protein 1 (AP-1) binding element via electrophoretic mobility shift assay and luciferase reporter assay. We determined whether the effect of halofuginone on collagen synthesis was dependent on the presence of c-Jun by ectopic expression of a wild-type or dominantnegative c-Jun construct in the presence of halofuginone and assaying ␣2(I) collagen promoter strength via luciferase reporter assay. The effect of halofuginone on ␣2(I) collagen message levels in fibroblasts when wildtype or dominant-negative c-Jun was overexpressed was determined. We also determined whether halofuginone Extracellular matrix (ECM) protein turnover is an important process in the maintenance of connective tissue structure. Consequently, there is a constant low level of collagen synthesis by fibroblasts to maintain the integrity of the ECM. During wound healing, there is a shift in the balance of ECM protein synthesis/degradation that favors an increased deposition of fibrotic material. In the normal state, this shift in the balance is short-lived. In fibrotic disease, however, there appears to

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“…Some are residual of cycles preceding the ongoing pregnancy, others result from the ovulations leading to pregnancy [5][6][7][8],…”

Section: Introductionmentioning

confidence: 99%

Evolution of NMRI Mice Ovaries and Corpora lutea during Pregnancy: Morphologic and Morphometric Study

Psalti

Loumaye

Rahier³

et al. 1988

Gynecol Obstet Invest

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During pregnancy in mice, three groups of corpora lutea (CL) originating from 4 successive ovulatory cycles could be distinguished taking into account their size, cellular structure and stain affinity. Type I CL originated at the onset of pregnancy, type II CL originated during the 2 previous estrous cycles and type III CL were produced during a cycle preceding the two others. CL volume of type I increased 10-fold between day 0 and day 18.5, a 2.5-fold increase in volume occurred in type II CL between day 0 and day 3, although they derived from cycles preceding pregnancy. The volume of type III remained unchanged. This fact suggested that types I and II CL probably play a functional role in the ongoing pregnancy. After day 10 of pregnancy a high correlation coefficient (r = 0.933) is observed between type I CL and the number of embryos in the corresponding uterine horn.

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Immunocytochemical studies of relaxin in ovaries of pregnant and cycling mice.

Cited by 12 publications

References 11 publications

Changes of Relaxin Concentrations Determined by Immunodensitometry in Ovaries of NMRI Mice during Pregnancy

Changes of Relaxin Concentrations Determined by Immunodensitometry in Ovaries of NMRI Mice during Pregnancy

Halofuginone inhibition of COL1A2 promoter activity via a c‐Jun–dependent mechanism

Evolution of NMRI Mice Ovaries and Corpora lutea during Pregnancy: Morphologic and Morphometric Study

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