Immunocytochemical study of Ki‐67 as a prognostic marker in canine mammary neoplasia

Zuccari, Débora A.P.C.; Santana, Áureo Evangelista; Cury, Patrí­cia Maluf; Cordeiro, José Antônio

doi:10.1111/j.1939-165x.2004.tb00345.x

Cited by 40 publications

(38 citation statements)

References 15 publications

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“…This result confirms literature data reporting Ki-67 as a marker for carcinomas and a marker of unfavorable prognosis (Zuccari et al 2004). PCNA increases after the G1 phase of the cell cycle, reaching a maximum peak in the S phase and decreasing after G2, and then presents very low levels in the M phase and in quiescent cells which are not detected by immunohistochemical methods (Tsuji et al 1992, Huang et al 1994, Rabenhorst et al 1994, Martinez-Lara et al 1996.…”

Section: Discussionsupporting

confidence: 79%

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Immunohistochemical evaluation of e-cadherin, Ki-67 and PCNA in canine mammary neoplasias: correlation of prognostic factors and clinical outcome

et al. 2008

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RESUMO.-[Avaliação imuno-histoquímica da e-caderina, Ki-67 e PCNA nas neoplasias mamárias caninas: correlação dos fatores prognósticos com a evolução clínica.] A e-caderina é uma molécula de adesão celular e a perda de sua expressão esta relacionada à invasão tumoral podendo indicar um prognóstico ruim nas neoplasias mamárias. A expressão dos marcadores de proliferação celular PCNA e especialmente o Ki-67, também têm mostrado forte valor prognóstico nesta classe tumoral. A expressão imuno-histoquímica destes marcadores foi relacionada com as características clinico-patológicas de 73 tumores removidos cirurgicamente de fêmeas caninas. Não houve correlação estatística entre estes marcadores E-cadherin is a cell-cell adhesion molecule and low e-cadherin expression is related to invasiveness and may indicate a bad prognosis in mammary neoplasms. The expression of cell proliferation markers PCNA and especially Ki-67, has also proved to have a strong prognostic value in this tumor class. The expression of these markers was related to the clinical-pathological characteristics of 73 surgically removed mammary tumors in female dogs by immunohistochemistry. There was no statistical correlation between these markers and death by neoplasm, survival time and disease-free interval. However, the loss of e-cadherin expression and marked Ki-67 expression (p=0.016) were considered statistically significant for the diagnosis (p=0.032). When evaluated as independent factors, there was evidence of the relationship between the loss of e-cadherin expression and high PCNA expression with changes in the body status (divided into obese, normal and cachectic) of female dogs (p=0.030); there was also evidence of the relationship between pseudopregnancy and e-cadherin alone (p=0.021) and for ulceration and PCNA alone (p=0.035). The significant correlation between the markers expression and these well known prognostic factors used individually or in combination suggests their prognostic value in canine mammary tumors.INDEX TERMS: Canine, cell proliferation marker, e-cadherin, immunohistochemistry, mammary neoplasm.Pesq. Vet. Bras. 28(4):207-215, abril 2008 Debora A.P.C. Zuccari et al. 208 e a morte por neoplasia, tempo de sobrevida e intervalo livre de doença. Entretanto, a perda da expressão da ecaderina e a forte expressão do 016) foram considerados estatisticamente significativos quando relacionados com o diagnóstico (p=0,032). Quando avaliados os fatores independentes, houve evidência de associação entre a perda de expressão da e-caderina e a alta expressão do PCNA com as mudanças no estado nutricional das cadelas (divididas em obesas, normais e caquéticas) (p=0,030); houve também evidência de associação entre a pseudociese e a expressão da e-caderina (p=0,021) e com a ulceração e a expressão do PCNA (p=0,035). A correlação significativa entre a expressão dos marcadores e estes, bem conhecidos fatores prognósticos usados individualmente ou em associação, sugere importante valor prognóstico destes marcadores nos tumores mamários cani...

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Section: Discussionsupporting

confidence: 79%

“…More recently, other authors have tried to relate cell proliferation markers with the diagnosis, prognosis and outcome of breast neoplasms (Pena et al 1998, Zacchetti et al 2003, Kushlinskii et al 2004, Zuccari et al 2004, Nowak et al 2006. Nowak et al (2006) confirmed the relationship between Ki-67 and PCNA.…”

Section: Discussionmentioning

confidence: 99%

Immunohistochemical evaluation of e-cadherin, Ki-67 and PCNA in canine mammary neoplasias: correlation of prognostic factors and clinical outcome

et al. 2008

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“…[2][3][4][5][6]8,16 In veterinary medicine, Ki-67 expression has been described as a proliferation marker and of predictive value for survival of animals with tumors of several nonnervous tissues. [13][14][15][17][18][19][20][21][22][23] It has also been used as a proliferation marker in various intracranial neoplasms including meningiomas in dogs. 24-27,a Angiogenesis is the process leading to the formation of new vessels from a pre-existing vascular network, and is essential for the growth and invasion of neoplasms.…”

mentioning

confidence: 99%

Ki‐67 and Vascular Endothelial Growth Factor Expression in Intracranial Meningiomas in Dogs

Matiasek¹,

Platt²,

Adams³

et al. 2009

Veterinary Internal Medicne

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Background: Tumor proliferation in human intracranial meningiomas can be defined by the reactivity of the monoclonal antibody MIB-1 to the Ki-67 antigen. Vascular endothelial growth factor (VEGF), a pro-angiogenic factor, is a predictive marker for survival of dogs with intracranial meningiomas.Hypothesis: Ki-67 is expressed in canine intracranial meningiomas and is associated with VEGF expression. Ki-67 expression is a prognostic marker for patient outcome.Animals: Seventy client-owned dogs with WHO grade I intracranial meningiomas. Methods: Retrospective study assessing the degree of immunostaining for Ki-67 by MIB-1 and VEGF expression in intracranial meningioma tissue from dogs. MIB-1 Labeling Index (LI) was calculated with Image J NIH-software. Extent, intensity, and distribution of VEGF-expression was assessed semiquantitatively. Cross tabulations with Fisher's exact tests and nonparametric Spearman's rank correlations were performed to identify associations between VEGF expression and MIB-1 LI. Fifteen dogs underwent postsurgical radiotherapy and were included in survival analysis. The effect of MIB-1 LI on survival was examined by Kaplan-Meier and Cox proportional hazards regression procedures.Results: Ki-67 staining was positive in 91% (64/70) and VEGF expression was detected in 96% (67/70). There was no significant association between VEGF expression and MIB-1 LI. MIB-1 LI was not associated with survival.Conclusions and Clinical Importance: MIB-1 antibody can be used to document cell proliferation in intracranial meningiomas in dogs, but does not predict outcome. No association between VEGF as a marker of angiogenesis and tumor proliferation was found. Angiogenesis might be a more important predictor of meningioma activity in dogs than is Ki-67.

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“…For this reason, antibodies against Ki-67 are increasingly being used in human and veterinary oncology as prognostic markers in different types of neoplasms. MIB-1 is the monoclonal antibody developed against the antigen that can be used on routinely formalinfixed, paraffin-wax embedded tissues after microwave antigen retrieval (12 (67,73,94,115). However, one study dealing with Ki-67 immunolabelling in canine mammary tumours reported lack of prognostic significance of this marker (52).…”

Section: Ki-67mentioning

confidence: 99%

Prognostic and predictive markers in canine tumours: Rationale and relevance. A review

Mukaratirwa¹

2005

Veterinary Quarterly

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Immunocytochemical study of Ki‐67 as a prognostic marker in canine mammary neoplasia

Cited by 40 publications

References 15 publications

Immunohistochemical evaluation of e-cadherin, Ki-67 and PCNA in canine mammary neoplasias: correlation of prognostic factors and clinical outcome

Immunohistochemical evaluation of e-cadherin, Ki-67 and PCNA in canine mammary neoplasias: correlation of prognostic factors and clinical outcome

Ki‐67 and Vascular Endothelial Growth Factor Expression in Intracranial Meningiomas in Dogs

Prognostic and predictive markers in canine tumours: Rationale and relevance. A review

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