Immunocytochemical visualization of the centromeres during male and female meiosis in Lilium longiflorum

Suzuki, Tatsujiro; Ide, Noriko; Tanaka, Ichiro

doi:10.1007/s004120050265

Cited by 31 publications

(19 citation statements)

References 53 publications

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“…For histone H4 immunoanalyses, microspores were fixed and permeabilised according to Suzuki et al (1997). Binucleate pollen protoplasts were cytocentrifuged on poly-L-lysine-coated slides with a nonextracting lysis/fixation mixture (2% formaldehyde, 2% Triton X-100) at 500 g for 20 min, the slides were immediately dipped in cold methanol and stored in 50% glycerol at 4 ~ until used (Hladitova et al 1998).…”

Section: Methodsmentioning

confidence: 99%

Histone H4 acetylation and DNA methylation dynamics during pollen development

2000

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Summary.The first pollen mitosis results in generative and vegetative cells which are characterised by a striking difference in their chromatin structure. In this study, histone H4 acetylation and DNA methylation have been analysed during pollen development in Lilium longiflorum. Indirect immunofiuorescence procedures followed by epiftuorescence and taser scanning microscopy enabled a relative quantification of H4 acetylation and DNA methylation in microspores, immature binucleate pollen, mature pollen, and pollen tubes. The results show that histone H4 of the vegetative nucleus, in spite of its decondensed chromatin structure, is strongly hypoacetylated at lysine positions 5 and 8 in comparison with both the original microspore nucleus and the generative-cell nucleus. These H4 terminal lysines in the vegetative nucleus are, however, progressively acetylated during the following pollen tube growth. The DNA methylation analysis inversely correlates with the histone acetylation data. The vegetative nucleus in mature pollen grains is heavily methylated, but a dramatic nonreplicative demethylation occurs during the pollen tube development. Changes neither in H4 acetylation nor in DNA methylation have been found during development of the generative nucleus. The results obtained indicate that the vegetative nucleus enters the quiescent state (accompanied by DNA hypermethylation and H4 underacetylation) during the maturation of pollen grain which enables pollen grains a long-term survival without external source of nutrients until they reach the stigma.

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Section: Methodsmentioning

confidence: 99%

Histone H4 acetylation and DNA methylation dynamics during pollen development

2000

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“…As prometaphase progresses into metaphase, the separation of sister kinetochores becomes more and more distinct, such that they can often be fully resolved from each other in the light microscope at anaphase (Goldstein, 1981;Suzuki et al, 1997;Dawe, 1998;Dawe et al, 1999). Sister kinetochore separation at meiosis I has never been demonstrated at the electron microscope level in plants.…”

Section: General Morphology Of Maize Meiotic Spindle and Kinetochoresmentioning

confidence: 99%

The simple ultrastructure of the maize kinetochore fits a two-domain model

Dawe

Richardson

Zhang

2005

Cytogenet Genome Res

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Light microscope observations suggest there are two discrete biochemical domains in the plant kinetochore, an inner domain containing structural proteins, and an outer domain containing proteins involved in motility. We analyzed the ultrastructure of maize meiotic kinetochores following high pressure freezing and freeze substitution, a method that provides excellent sample preservation. Data from meiosis II support previous descriptions of plant kinetochores as diffuse, nearly invisible domains, sometimes nesting in a cup of darkly staining chromatin. The ultrastructure is similar in meiosis I but there are two sister kinetochores that each protrude away from the chromosome and form their own distinct kinetochore fibers. Microtubules terminate within kinetochores where their ends are splayed in a cone-shaped configuration suggestive of microtubule disassembly. We could not detect any significant substructure within the kinetochore proper. We suggest that the diffuse structure classically defined as the kinetochore represents only the outer domain of a two-domain organelle. The inner domain, known to contain chromatin-binding proteins, probably extends into the electron-dense chromatin of the primary constriction.

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Section: Centromere Condensation and Structurementioning

confidence: 99%

“…In hexaploid bread wheat, the Ph1 locus controls both meiotic centromere decondensation and homeologous pairing (Aragón-Alcaide et al, 1997). Meiotic condensation in the Easter lily may be regulated by meiosis-specific H1 histones (Hasenkampf et al, 2000), including a centromere-specific form (Suzuki et al, 1997).In interphase root tip cells, at least some of the centromeres show a discontinuous bead-like distribution of HTR12 protein, which we interpret as extreme decondensation of centromeric chromatin. This pattern is quite similar to the beaded pattern of prekinetochore staining detected in interphase Indian muntjac cells (Brinkley et al, 1984) or the patterns of staining of stretched metaphase kinetochores from several species, including the African blood lily (Zinkowski et al, 1991).…”

mentioning

confidence: 99%

Centromeric Localization and Adaptive Evolution of an Arabidopsis Histone H3 Variant

et al. 2002

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Centromeric H3-like histones, which replace histone H3 in the centromeric chromatin of animals and fungi, have not been reported in plants. We identified a histone H3 variant from Arabidopsis thaliana that encodes a centromere-identifying protein designated HTR12. By immunological detection, HTR12 localized at centromeres in both mitotic and meiotic cells. HTR12 signal revealed tissue-and stage-specific differences in centromere morphology, including a distended bead-like structure in interphase root tip cells. The anti-HTR12 antibody also detected spherical organelles in meiotic cells. Although the antibody does not label centromeres in the closely related species Arabidopsis arenosa , HTR12 signal was found on all centromeres in allopolyploids of these two species. Comparison of the HTR12 genes of A. thaliana and A. arenosa revealed striking adaptive evolution in the N-terminal tail of the protein, similar to the pattern seen in its counterpart in Drosophila . This finding suggests that the same evolutionary forces shape centromeric chromatin in both animals and plants. INTRODUCTIONCentromeres are the specialized chromosomal sites necessary for poleward movement during mitosis and meiosis in eukaryotes. Commonly, a centromere is evident as a prominent constriction within the heterochromatin of each metaphase chromosome. The attachment to and movement of chromosomes along the spindle is mediated by the proteinaceous kinetochores, which form at the centromeres during cell division.Despite this highly conserved function, centromeric DNA sequences are not conserved between organisms. For example, human centromeres consist of large blocks (200 kb to several megabases) of tandemly repeated 171-bp ␣ -satellite (Willard, 1998), but the sequences can differ from those of apes on homologous chromosomes (Haaf and Willard, 1997). Similarly, Drosophila melanogaster centromeric regions contain blocks of 5-to 12-bp satellite repeats that do not appear to be shared by homologous centromeres of sibling species (Lohe and Brutlag, 1987).Plant centromeric regions resemble their mammalian counterparts in that both have large arrays of tandem repeats, frequently of approximately nucleosomal size. In centromere regions of maize (Alfenito and Birchler, 1993;Ananiev et al., 1998), pearl millet (Kamm et al., 1994), rice (Dong et al., 1998), sugarcane (Nagaki et al., 1998), sorghum (Zwick et al., 2000), the Australian daisy Brachycome dichromosomatica (Leach et al., 1995), rape (Xia et al., 1993), and the wild beet Beta procumbens (Gindullis et al., 2001), tandem repeats have been found that differ in sequence but that all have lengths in the range of ‫ف‬ 140 to 180 bp. Particular repeat arrays in cereals have been estimated to be hundreds of kilobases long (Dong et al., 1998;Kaszás and Birchler, 1998). Cereal tandem repeat arrays are interrupted by TY3/gypsy -like retrotransposons and other retrotransposons (Ananiev et al., 1998;Kumekawa et al., 2001;Nonomura and Kurata, 2001). The satellite arrays and interspersed retrotransposons o...

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Immunocytochemical visualization of the centromeres during male and female meiosis in Lilium longiflorum

Cited by 31 publications

References 53 publications

Histone H4 acetylation and DNA methylation dynamics during pollen development

Histone H4 acetylation and DNA methylation dynamics during pollen development

The simple ultrastructure of the maize kinetochore fits a two-domain model

Centromeric Localization and Adaptive Evolution of an Arabidopsis Histone H3 Variant

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