Immunodetection and Quantification of<i>Botrytis cinerea</i>on Harvested Wine Grapes

Ricker, R. W.; Marois, James J.; Dlott, J. W.; Bostock, Richard M.; Morrison, J. C.

doi:10.1094/phyto-81-404

Cited by 34 publications

(21 citation statements)

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“…Gray mold susceptibility, caused by B. cinerea, was detected by visual inspection, by an experienced technician, of the fruits on plants during the harvesting season and on the fruit samples stored at 4°C for 72 h. Varieties were scored, based on a subjective index of conidia development, in a range from 0, when no development of the fungus was present, to 5, when the attack was severe. Although this procedure is highly dependent on the skills of the inspector and is possible only when the fungus is sporulating, it is the most widely used in recording gray mold (22).…”

Section: Methodsmentioning

confidence: 99%

Characterization of 14 Raspberry Cultivars by Solid-Phase Microextraction and Relationship with Gray Mold Susceptibility

Aprea

Carlin²,

Giongo³

et al. 2010

J. Agric. Food Chem.

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Fourteen raspberry varieties were evaluated over two cropping seasons by solid-phase microextraction (SPME) followed by gas chromatography-mass spectrometry. Thirty-six compounds were fully identified, and 10 more compounds were tentatively identified. Despite interannual variability, raspberry varieties can be divided in two main groups on the basis of terpenes and C-13 norisoprenoids. Susceptibility toward Botrytis cinerea , one of the most relevant pathogenic fungi for soft fruits during storage, was also evaluated. On the basis of volatile profiles, it was possible to highlight the relationship between different volatile compounds and resistance to B. cinerea. Volatile profiles and Botrytis susceptibility of the different raspberry varieties evaluated should assist future breeding programs.

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Section: Methodsmentioning

confidence: 99%

Characterization of 14 Raspberry Cultivars by Solid-Phase Microextraction and Relationship with Gray Mold Susceptibility

Aprea

Carlin²,

Giongo³

et al. 2010

J. Agric. Food Chem.

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“…PGIP activity assay B. cinerea DEL 11 (obtained from Richard M. Bostock, Department of Plant Pathology, University of California, Davis) was grown in modified Czapek medium [31] containing 2~o (w/v) glucose and 3~o (w/v) pear cell walls [12] on a rotary shaker at 125 rpm. After 17 d the culture medium was collected by filtration through cheesecloth and Whatman 1 MM paper and concentrated by ultrafiltration using a PM-10 membrane.…”

Section: Protein Purificationmentioning

confidence: 99%

Structure and expression of an inhibitor of fungal polygalacturonases from tomato

et al. 1994

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A polygalacturonase inhibitor protein (PGIP) was characterized from tomato fruit. Differential glycosylation of a single polypeptide accounted for heterogeneity in concanavalin A binding and in molecular mass. Tomato PGIP had a native molecular mass of 35 to 41 kDa, a native isoelectric point of 9.0, and a chemically deglycosylated molecular mass of 34 kDa, suggesting shared structural similarities with pear fruit PGIP. When purified PGIPs from pear and tomato were compared, tomato PGIP was approximately twenty-fold less effective an inhibitor of polygalacturonase activity isolated from cultures of Botrytis cinerea. Based on partial amino acid sequence, polymerase chain reaction products and genomic clones were isolated and used to demonstrate the presence of PGIP mRNA in both immature and ripening fruit as well as cell suspension cultures. Nucleotide sequence analysis indicates that the gene, uninterrupted by introns, encodes a predicted 36.5 kDa polypeptide containing amino acid sequences determined from the purified protein and sharing 68% and 50% amino acid sequence identity with pear and bean PGIPs, respectively. Analysis of the PGIP sequences also revealed that they belong to a class of proteins which contain leucine-rich tandem repeats. Because these sequence domains have been associated with protein-protein interactions, it is possible that they contribute to the interaction between PGIP and fungal polygalacturonases.

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“…This could be because they are present in all cellular organisms and contain highly conserved and moderately antigenically simpler proteins. Other workers (Musgrave et al, 1984;Mohan, 1988;Harrison et al, 1990;Dewey et al, 1991;Velicheti, 1993) also demonstrated that polyclonal antisera raised against one fungal species are often genus specific and specificity can rarely be improved through the cross-absorption technique (Ricker et al, 1991).…”

Section: Discussionmentioning

confidence: 99%

Evaluation of a polyclonal antibody immunoassay for detection and quantification of Macrophomina phaseolina

Srivastava

Arora

1997

Plant Pathology

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Detection and quantification of Macrophomina phaseolina, causal agent of charcoal/dry root rot disease in many crop plants, was carried out using the ELISA serological technique. Polyclonal antisera raised to water soluble extracts of mycelium, the residual water insoluble mycelial materials or ribosomal proteins were evaluated for specificity and cross-reactivity with 16 common soil fungi by ODD and DAS-ELISA. Soluble and cell wall antisera exhibited strong cross-reactivity with most of the fungal isolates. Ribosomal antibodies were less reactive to common soil fungi except Fusarium oxysporum f.sp. ciceri. Mycelial antigens of M. phaseolina on chickpea roots were detectable with DAS-ELISA at a minimum concentration of 10 ng g ¹1 at 1 : 100 root : buffer dilution. Quantitative estimation of M. phaseolina on roots was evaluated by ELISA under different temperatures and moisture conditions, and in soil amended with a potential antagonist (Trichoderma harzianum 25-92). A significant reduction in ELISA values was observed in T. harzianumamended treatments. This method may be useful for detection and rapid screening of M. phaseolina under different environmental conditions.

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Immunodetection and Quantification ofBotrytis cinereaon Harvested Wine Grapes

Cited by 34 publications

References 0 publications

Characterization of 14 Raspberry Cultivars by Solid-Phase Microextraction and Relationship with Gray Mold Susceptibility

Characterization of 14 Raspberry Cultivars by Solid-Phase Microextraction and Relationship with Gray Mold Susceptibility

Structure and expression of an inhibitor of fungal polygalacturonases from tomato

Evaluation of a polyclonal antibody immunoassay for detection and quantification of Macrophomina phaseolina

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