Immunodetection of estrogen receptors in fetal and neonatal male mouse reproductive tracts

Greco, Tamara L.

doi:10.1210/en.130.1.421

Cited by 42 publications

(33 citation statements)

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“…Uterine stromal cells stained for ER were observed at all ages examined, but staining appeared to decrease with age in the control animals and with DES treatment. Similar results have been reported in male (18) and female (19) mouse reproductive tract tissues following neonatal exposure to DES (20). Additional information on the role of ER in embryonic tissues has been described by Gorski and Hou (21).…”

Section: Murine Model Of Developmental Exposure To Diethylstilbestrolsupporting

confidence: 86%

Cellular and molecular effects of developmental exposure to diethylstilbestrol: implications for other environmental estrogens.

Newbold

1995

Environ Health Perspect

185

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Concerns have been raised regarding the role of environmental and dietary estrogens as possible contributors to an increased incidence of various abnormalities in estrogen-target tissues of both sexes. These abnormalities include breast cancer, endometriosis, fibroids, and uterine adenocarcinoma in females, as well as alterations in sex differentiation, decreased sperm concentrations, benign prostatic hyperplasia, prostatic cancer, testicular cancer, and reproductive problems in males. Whether these concerns are valid remains to be determined; however, studies with the potent synthetic estrogen diethylstilbestrol (DES) suggest that exogenous estrogen exposure during critical stages of development can result in permanent cellular and molecular alterations in the exposed organism. These alterations manifest themselves in the female and male as structural, functional, or long-term pathological changes including neoplasia. Although DES has potent estrogenic activity, it may be used as a model compound to study the effects of weaker environmental estrogens, many of which may fit into the category of endocrine disruptors. -Environ Health Perspect 103(Suppl 7): 83-87 (1995)

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Section: Murine Model Of Developmental Exposure To Diethylstilbestrolsupporting

confidence: 86%

Cellular and molecular effects of developmental exposure to diethylstilbestrol: implications for other environmental estrogens.

Newbold

1995

Environ Health Perspect

185

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“…It differs from other histological investigations of oestrogen receptor expression in mice during this period (Stumpf et al, 1980;Holderegger and Keefer, 1986;Cooke et al, 1991;Greco et al, 1991Greco et al, , 1992 in the following ways. First, the use of oestrogen receptor-specific antibodies rather than radiolabelled ligands (that is, […”

Section: Discussionmentioning

confidence: 70%

“…However, these studies have limitations in histological interpretation as a result of the use of either autoradiography (Stumpf et al, 1980;Schleicher et al, 1984;Holderegger and Keefer, 1986;Cooke et al, 1991) or frozen sections (Greco et al, 1991(Greco et al, , 1992. Furthermore, these studies do not discriminate between ER-α and ER-β.…”

Section: Introductionmentioning

confidence: 99%

Ontogeny of oestrogen receptor alpha in gonads and sex ducts of fetal and newborn mice

Nielsen¹,

Björnsdóttir²,

Hoyer³

et al. 2000

Reproduction

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“…In previous studies from this laboratory, Greco et al. (16)(17)(18) showed that ER is present in both male and female mice from gestational day 10 and later, with definite evidence of ER being present in reproductive tissues at gestational day 1 or later. This includes the gonads (Figure 3) (18) Currently there is a great deal of interest in environmental estrogens that originate from sources such as chemical processing or foods that contain mycotoxins or phytoestrogens.…”

Section: Environmental Health Perspectives Embryonic Estrogen Receptorsmentioning

confidence: 92%

Embryonic estrogen receptors: do they have a physiological function?

Gorski

Hou

1995

Environ Health Perspect

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In normal estrogen target tissues, estrogen action is mediated through a specific nuclear transcription factor, the estrogen receptor (ER). The site of estrogen action in the developing organism is therefore determined by cells that contain ER and other necessary tissue and gene-specific components for estrogen-mediated transcription. Immunocytochemical methods were used to determine the cellular localization and tissue distribution of ERs in reproductive tracts of mouse fetuses. Nuclear staining for ER was observed in reproductive tracts at fetal days 13 to 15. ERs were present in the precursors of both male and female reproductive tracts at these early developmental stages, which may be attributable to their similar embryonic origins. However, as the tissues undergo sexual differentiation at later fetal and early neonatal ages, ER increases in the female reproductive tracts as compared with the male. ER was detected by immunoblotting on fetal day 10 (before sexual differentiation) in extracts of whole mouse embryos. To determine whether ER and progesterone receptor genes are expressed earlier in development, we examined RNA from preimplantation mouse embryos using reverse transcriptase-polymerase chain reaction techniques. ER mRNA was found in oocytes and fertilized eggs. Message concentration declined at the 2-cell stage and reached its lowest level at the 5-to 8-cell stage. ER mRNA was not detectable at the morula stage but reappeared at the blastocyst stage. Progesterone receptor mRNA was not detectable until the blastocyst stage. The embryonic expression of ER and progesterone receptor genes in the blastocyst suggests a possible functional requirement for estrogen and progesterone receptors in preimplantation embryos. -Environ Health Perspect 1 03(Suppl 7):69-72 (1995)

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Immunodetection of estrogen receptors in fetal and neonatal male mouse reproductive tracts

Cited by 42 publications

References 0 publications

Cellular and molecular effects of developmental exposure to diethylstilbestrol: implications for other environmental estrogens.

Cellular and molecular effects of developmental exposure to diethylstilbestrol: implications for other environmental estrogens.

Ontogeny of oestrogen receptor alpha in gonads and sex ducts of fetal and newborn mice

Embryonic estrogen receptors: do they have a physiological function?

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