Immunodetection of pentamer and modified C-reactive protein using surface plasmon resonance biosensing

Sensitive detection of C-reactive protein using optical fiber Bragg gratings

“…The photometric methods like ELISA can be affected by the colour and composition of the medium used in assays (Meyer et al, 2006). Many other methods such as surface plasmon resonance (SPR) (Meyer et al, 2006;Hu et al, 2006), quartz crystal microbalance (QCM) (Kim et al, 2009), piezoelectric micro-cantilever method (Wee et al, 2005;Gan et al, 2012), electrochemistry (Centi et al, 2009;Buch and Rishpon, 2008), electrochemical impedance spectroscopy (EIS) (Bryan et al, 2013) and micro particle-tracking velocimetry (MPTV) (Fan et al, 2010) have been developed for CRP detection. Laser induced fluorescence (Lin et al, 2013) and chemiluminescence (Islam and Kang, 2011) methods have also used for the detection of CRP.…”

Biosensors for Cancer Biomarkers

“…Weinhold and Rüther, 1997). Plasma levels are lower than normal human, 3μg/mL (Hu et al, 2006;Verma and Yeh, 2003). Used to determine levels of CRP as a prognostic indicator of gastric cancer.…”

Aptamer–antibody on-chip sandwich immunoassay for detection of CRP in spiked serum

“…The latter offer the unique advantage of highly parallel assay format and high throughput capacity. Most of the on-chip immunoassays reported in the literature use the sandwich format and perform detection either by absorbance (Christodoulides et al, 2002), fluorescence (Shin et al, 2007;Wolf et al, 2004;Abdo et al, 2005) or SPR (Hu et al, 2006;Meyer et al, 2006;Vikholm-Lundin and Albers, 2006;Bini et al, 2008). The assay principle is usually based on the formation of an antibody-antigen complex.…”

“…Detection of CRP on planar surfaces may be performed by classic ELISA or using biochips, including both those which function under "microfluidic" conditions (kinetic: Shin et al, 2007;Wolf et al, 2004;Hu et al, 2006;Meyer et al, 2006;Christodoulides et al, 2002;Roper et al, 2006) and others which do not (static measurement: Gul et al, 2007;Abdo et al, 2005). The latter offer the unique advantage of highly parallel assay format and high throughput capacity.…”