Immunodiagnosis of angiostrongyliasis with monoclonal antibodies recognizing a circulating antigen of mol.wt 91,000 from Angiostrongylus cantonensis

Shih, Hsiu-Hui; Chen, S.N.

doi:10.1016/0020-7519(91)90007-t

Cited by 19 publications

(9 citation statements)

References 18 publications

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“…Various circulating antigens of A. cantonensis in human angiostrongyliasis sera and CSF (and sera of infected animals) had been demonstrated by sandwich ELISA (Eamsobhana et al, 1995(Eamsobhana et al, , 1997Chye et al, 1997;Chen et al, 2011), dot-blot ELISA (Eamsobhana et al, 1997), enzyme-linked fluorescent assay (Shih and Chen, 1991), lateral flow immunoassay (Chen et al, 2016), ELISA and Western blotting (Liang et al, 2005).…”

Section: Resultsmentioning

confidence: 99%

Sandwich dot-immunogold filtration assay (DIGFA) for specific immunodiagnosis of active neuroangiostrongyliasis

et al. 2020

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Serological tests may yield false-negative results for specific antibodies detection before or at the early seroconversion phase. Tests that detect circulating antigens of Angiostrongylus cantonensis would therefore be of value in diagnosis to distinguish current or past infection. Here, a quick, easy to perform, portable and inexpensive diagnostic device for detection of 31-kDa A. cantonensis specific antigens had been developed. This sandwich dot-immunogold filtration assay (AcDIGFAAg), for detecting active angiostrongyliasis was produced using anti-A. cantonensis polyclonal antibody dotted on the nitrocellulose membrane as a capture agent and colloidal gold-labelled anti-31 kDa A. cantonensis antibody as a detection agent. A well-defined pink dot, indicating positivity, was seen readily by naked eye within 10–15 min. The AcDIGFAAg detected A. cantonensis-specific antigens in cerebrospinal fluid samples from 4 out of 10 serologically confirmed angiostrongyliasis cases and 2 out of 5 suspected cases with negative anti-A. cantonensis antibodies. Among the 19 patient sera with A. cantonensis infection, 2 showed positive reaction by AcDIGFAAg. No positive AcDIGFAAg reaction was observed in all the serum samples with other parasitic diseases, and the healthy controls. The present ‘AcDIGFAAg’ enables rapid qualitative detection of the specific 31-kDa antigens of A. cantonensis in clinical samples with potential for application even under resource-limited settings.

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Section: Resultsmentioning

confidence: 99%

Sandwich dot-immunogold filtration assay (DIGFA) for specific immunodiagnosis of active neuroangiostrongyliasis

et al. 2020

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“…High sensitivity (91%) and specificity (98%) for detecting serum antibodies in patients with eosinophilic meningitis or meningoencephalitis have been obtained by an ELISA incorporating a purified AcL 5 antigen with a molecular mass of 204 kDa and a corresponding mAb (17 ). However, delays in the appearance of antibodies after infestation and the persistence of antibodies after cure have restricted the diagnostic reliability of serum antibody detection in patients with these parasitic diseases (18 ).…”

Section: Discussionmentioning

confidence: 99%

“…The purification of these antigens, typically accomplished by column chromatography, is a time-consuming process because crude antigens typically require passage through several columns to appropriately prepare them for immunodiagnostic use. In another approach, specific monoclonal antibodies (mAbs) against antigens of the AcL 3 or AcL 5 larval stages of A. cantonensis have been prepared and used to detect circulating antigen in a double-antibody sandwich ELISA (18,19 ). The specificity of this method is high, but the detection sensitivity remains unsuitably low.…”

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confidence: 99%

Immuno-PCR for Detection of Antigen to Angiostrongylus cantonensis Circulating Fifth-Stage Worms

Chye

Lin²,

Chen

et al. 2004

Clinical Chemistry

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Background: Definitive diagnosis of infestation withAngiostrongylus cantonensis is difficult because the parasitic nematode is undetectable in the cerebrospinal fluid (CSF) of one-half of afflicted patients and the diagnostic sensitivity of ELISA for circulating worm antigens in patient sera is low. We studied immuno-PCR as a diagnostic tool. Methods: We studied 30 controls and 60 afflicted patients (30 confirmed by parasitologic analysis of CSF).We used a monoclonal antibody to capture circulating A. cantonensis antigens in serum samples. A DNA label generated by PCR amplification with biotinylated primer was bound by use of streptavidin to a biotinylated third antibody. Circulating antigens sandwiched by monoclonal antibody were detected by PCR amplification of the DNA label. Results: The detection limit of the ELISA was 100 -1000 times higher than that of the immuno-PCR. The concentrations of circulating antigens in patients were markedly higher than those in controls (Wilcoxon rank-sum test, P <0.001). At a cutoff of 0.1 ng/L, sensitivity and specificity for immunodiagnosis of patients with angiostrongyliasis by immuno-PCR were 98% (95% confidence interval, 91-99%) and 100% (93-100%), respectively. The test was positive in all parasitologically confirmed cases. Conclusions: Immuno-PCR is a promising technique for diagnosis of A. cantonensis infestation.

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“…The time-resolved¯uorescent assay has also been used to detect CA in other parasitic diseases, e.g. schistosomiasis and angiostrongyliasis (Aceti et al 1988;de Jonge et al 1989;Shih and Chen 1991).…”

Section: Development Of Detection Systemmentioning

confidence: 99%

The detection and occurrence of circulating antigens of Trichinella spiralis during worm development

Li¹,

Ko²

2001

Parasitol Res

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Four enzyme-based immunoassays were compared in detecting circulating antigens (CA) of Trichinella spiralis, i.e. microfluorescence, dissociated enhanced lanthanide fluoroimmunoassay (DELFIA), enhanced chemiluminescence and enzyme-linked immunosorbent assay (ELISA). Parameters which could affect the sensitivity and specificity of the assays were evaluated. Different combinations of polyclonal antibody (PA) and five monoclonal antibodies (mAbs) against the excretory/secretory antigens of the nematode were tested to produce an optimal antigen-detecting system. DELFIA and a "sandwich" consisting of PA as capturing antibody and mAb 7C2C5 as detecting antibody, yielded the most stable and sensitive results; and 1 ng CA/ml could be detected. The assay did not cross-react with heterologous antigens of Angiostrongylus cantonensis, Ascaris suum, Cysticercus cellulosae, Fasciolopsis buski, Gnathostoma hispidum and Trichuris suis. Fluctuating levels of CA were observed in the serum of experimentally infected mice at various periods post-infection. As early as days 4 and 6, a significant amount of CA was detected. The level reached a peak at day 10, then declined and another peak was observed at day 18. The monitoring of the corresponding antibody response by ELISA showed that IgM was first detected at day 10, reaching a peak at day 16. A marked increase in IgG1 was noted from day 16 and its level was significantly higher than that of IgG2.

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Immunodiagnosis of angiostrongyliasis with monoclonal antibodies recognizing a circulating antigen of mol.wt 91,000 from Angiostrongylus cantonensis

Cited by 19 publications

References 18 publications

Sandwich dot-immunogold filtration assay (DIGFA) for specific immunodiagnosis of active neuroangiostrongyliasis

Sandwich dot-immunogold filtration assay (DIGFA) for specific immunodiagnosis of active neuroangiostrongyliasis

Immuno-PCR for Detection of Antigen to Angiostrongylus cantonensis Circulating Fifth-Stage Worms

The detection and occurrence of circulating antigens of Trichinella spiralis during worm development

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