Immunodiagnosis of Egyptian Human Fasciolosis Gigantica Using Fas1 and Fas2 Cysteine Proteinase Antigens

Rabee, I.; Mahana, Noha; Badr, Abeer M.

doi:10.12816/0006435

Cited by 5 publications

(4 citation statements)

References 12 publications

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“…Vaccination with cysteine protease coding DNAs induced significant protection against several helminth and protozoan infections by reducing the parasite burden or fecal egg count and reducing egg viability [ 37 ]. In addition, many cysteine proteases can be used for the immunodiagnosis of parasitic diseases with good sensitivity and specificity [ 38 , 39 ]. Recognition of the cysteine protease by early T. spiralis infection sera in this study suggests that T. spiralis cysteine protease is not only a good target for vaccine but also a promising target for immunodiagnostic antigen.…”

Section: Discussionmentioning

confidence: 99%

Immunoproteomic profile of Trichinella spiralis adult worm proteins recognized by early infection sera

et al. 2015

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BackgroundTrichinellosis, a widespread zoonosis, is regarded as an emerging or reemerging disease. Effective treatment and prognosis of trichinellosis depends on early diagnosis of the infection. The objective of this study was to identify sensitive and specific antigens for early diagnosis or effective vaccine antigens for preventing infection.MethodsThe somatic proteins of T. spiralis adult worms were separated by two-dimensional gel electrophoresis (2-DE). The separated proteins were probed with early infection sera from swine or mice infected with T. spiralis for 7 days. The primary immunoreactive spots were characterized by MALDI-TOF/TOF-MS analysis in combination with bioinformatics. The identified proteins were annotated using WEGO based on their functions. The immunodominant protein was chosen for expression as recombinant protein in E. coli and the purified recombinant protein was used to confirm its antigenicity by Western blot with the same infection sera.ResultsApproximately 300 spots were separated by 2-DE, with molecular weights ranging from 10 to 130 kDa, and pI values ranging from pH 4 to 10. The sera from swine and mice infected with T. spiralis for 7 days recognized 64 proteins. MALDI-TOF/TOF-MS analysis identified 55 proteins, some with different isoforms. Finally, 40 individual immunoreactive proteins were obtained with a wide range of biological functions. Several proteins, such as heat shock protein 70, 14-3-3 protein, and cysteine protease could be used as immunodiagnostic or vaccine antigens. Among these identified proteins, the highly immunodominant Ts14-3-3 was chosen for expression in E. coli and purified recombinant Ts14-3-3 was able to be strongly recognized by the same T. spiralis infected sera used for identifying these antigens, therefore the most promising antigen for early immunodiagnosis of Trichinella infection.ConclusionsA total of 64 proteins from the adult worm were recognized by early infection sera from swine and mice infected with T. spiralis for 7 days. Several proteins, are of particular interest as immunodiagnostic or vaccine antigens, especially with Ts14-3-3 as most promising due to its highly immunogenicity during early infection, expressed protein can be recognized by Trichinella early infection sera and the native Ts14-3-3 expression in both adult and larval stages.

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Section: Discussionmentioning

confidence: 99%

Immunoproteomic profile of Trichinella spiralis adult worm proteins recognized by early infection sera

et al. 2015

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“…They added that all ages and both sexes were susceptible to fascioliasis infection and that watercress topped the list of the Egyptian plants born encysted metacercariae followed by lettuce, mint, and alfalfa. No doubt, the establishment of rapid and dependable sero-diagnosis (Rabee et al, 2013), the control measures of snails intermediate hosts (Dar et al, 2014) and availability of treatment explained the absence of fascioliasis in the group.…”

Section: Discussionmentioning

confidence: 99%

Prevalence of Intestinal Parasites and Its Impact on Nutritional Status Among Preschool Children Living in Damanhur City, El-Behera Governorate, Egypt

Hegazy

Younis

Aminou

et al. 2014

Journal of the Egyptian Society of Parasitology

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“…. Similar ELISA tests are available for F. gigantica using a variety of different antigens: (a) Fas1 and Fas2 cysteine proteases, resulting in 91·9% and 89·1%, sensitivity and specificity, respectively ; (b) sandwich ELISA using fatty acid binding protein, resulting in 94·7% sensitivity and 84·62% specificities ; (c) 27 kDa circulating antigen from sera of infected individuals gave >93% sensitivity and specificity . A lateral flow test (SeroFluke) was developed by Martinez‐Sernandez and colleagues using samples obtained from F. hepatica‐ infected patients in Spain and Portugal, for use with serum or whole blood samples, which requires minimal training .…”

Section: Diagnosis Of Human Liver Fluke Infectionmentioning

confidence: 99%

A prospective view of animal and human Fasciolosis

Cwiklinski

O’Neill

Donnelly

et al. 2016

Parasite Immunology

171

114

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SummaryFasciolosis, a food‐borne trematodiasis, results following infection with the parasites, Fasciola hepatica and Fasciola gigantica. These trematodes greatly affect the global agricultural community, infecting millions of ruminants worldwide and causing annual economic losses in excess of US $3 billion. Fasciolosis, an important zoonosis, is classified by WHO as a neglected tropical disease with an estimated 17 million people infected and a further 180 million people at risk of infection. The significant impact on agriculture and human health together with the increasing demand for animal‐derived food products to support global population growth demonstrate that fasciolosis is a major One Health problem. This review details the problematic issues surrounding fasciolosis control, including drug resistance, lack of diagnosis and the threat that hybridization of the Fasciola species poses to future animal and human health. We discuss how these parasites may mediate their long‐term survival through regulation and modulation of the host immune system, by altering the host immune homeostasis and/or by influencing the intestinal microbiome particularly in respect to concurrent infections with other pathogens. Large genome, transcriptome and proteomic data sets are now available to support an integrated One Health approach to develop novel diagnostic and control strategies for both animal and human disease.

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Immunodiagnosis of Egyptian Human Fasciolosis Gigantica Using Fas1 and Fas2 Cysteine Proteinase Antigens

Cited by 5 publications

References 12 publications

Immunoproteomic profile of Trichinella spiralis adult worm proteins recognized by early infection sera

Immunoproteomic profile of Trichinella spiralis adult worm proteins recognized by early infection sera

Prevalence of Intestinal Parasites and Its Impact on Nutritional Status Among Preschool Children Living in Damanhur City, El-Behera Governorate, Egypt

A prospective view of animal and human Fasciolosis

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