Immunodiagnosis of human eosinophilic meningitis using an antigen of Angiostrongylus cantonensis L5 with molecular weight 204 kD

Chye, Soi-Moi; Chang, Jui‐Hung; Yen, Chuan-Min

doi:10.1016/s0001-706x(99)00082-0

Cited by 43 publications

(33 citation statements)

References 12 publications

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“…1). A diagnosis of A. cantonensis infection was made by a positive microenzymelinked immunosorbent assay (ELISA), using young-adult worm antigen molecular weight 204 kD, purified by monoclonal antibody (14).…”

Section: Introductionmentioning

confidence: 99%

Encephalitis Caused by Angiostrongylus cantonensis after Eating Raw Frogs Mixed with Wine as a Health Supplement

Tsai

Lai

et al. 2011

Intern. Med.

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Angiostrongylus cantonensis also known as the rat lungworm, is prevalent in the Pacific Islands and southeast Asia and is the most common cause of eosinophilic meningitis in humans. Although frogs and toads are known as paratenic hosts of A. cantonensis, they are rarely reported as the infectious source of human angiostrongyliasis. We report a case of encephalitis caused by Angiostrongylus cantonensis after eating raw frogs mixed with wine as a health supplement. Prednisolone at a dose of 1 mg/kg/day was prescribed for 14 days successfully. We advise that travelers and residents of endemic areas should avoid eating raw frogs and a public caution on the danger of eating raw wild animal products or the whole animal is recommended to alleviate such accidental infection.

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Section: Introductionmentioning

confidence: 99%

Encephalitis Caused by Angiostrongylus cantonensis after Eating Raw Frogs Mixed with Wine as a Health Supplement

Tsai

Lai

et al. 2011

Intern. Med.

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“…High sensitivity (91%) and specificity (98%) for detecting serum antibodies in patients with eosinophilic meningitis or meningoencephalitis have been obtained by an ELISA incorporating a purified AcL 5 antigen with a molecular mass of 204 kDa and a corresponding mAb (17 ). However, delays in the appearance of antibodies after infestation and the persistence of antibodies after cure have restricted the diagnostic reliability of serum antibody detection in patients with these parasitic diseases (18 ).…”

Section: Discussionmentioning

confidence: 99%

“…AcL 3 was collected from infested Biomphalaria glablata snails after they were mechanically minced and digested with use of artificial gastric juice (17 ). Hybrid mice were individually infested with 50 larvae via an oral stomach tube.…”

Section: Materials and Methods Antigen Preparationmentioning

confidence: 99%

“…AcJ1 secretes IgG2a, and AcJ20 secretes IgG1. Both antibodies recognize specific AcL 5 antigens with a molecular mass of 204 kDa (17 ). These hybridomas were injected into the peritoneum of pristane-primed BALB/c mice.…”

Section: Purification Of Mabsmentioning

confidence: 99%

“…Most methods have not displayed sufficient antigenic specificity to be of reliable diagnostic value. To avoid the diagnostic liability of antigenic cross-reactivity between A. cantonensis and other helminthic species, partially purified and purified A. cantonensis antigens have been used to detect angiostrongyliasis-affected patients (12)(13)(14)(15)(16)(17). The purification of these antigens, typically accomplished by column chromatography, is a time-consuming process because crude antigens typically require passage through several columns to appropriately prepare them for immunodiagnostic use.…”

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Immuno-PCR for Detection of Antigen to Angiostrongylus cantonensis Circulating Fifth-Stage Worms

Chye

Lin²,

Chen

et al. 2004

Clinical Chemistry

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Background: Definitive diagnosis of infestation withAngiostrongylus cantonensis is difficult because the parasitic nematode is undetectable in the cerebrospinal fluid (CSF) of one-half of afflicted patients and the diagnostic sensitivity of ELISA for circulating worm antigens in patient sera is low. We studied immuno-PCR as a diagnostic tool. Methods: We studied 30 controls and 60 afflicted patients (30 confirmed by parasitologic analysis of CSF).We used a monoclonal antibody to capture circulating A. cantonensis antigens in serum samples. A DNA label generated by PCR amplification with biotinylated primer was bound by use of streptavidin to a biotinylated third antibody. Circulating antigens sandwiched by monoclonal antibody were detected by PCR amplification of the DNA label. Results: The detection limit of the ELISA was 100 -1000 times higher than that of the immuno-PCR. The concentrations of circulating antigens in patients were markedly higher than those in controls (Wilcoxon rank-sum test, P <0.001). At a cutoff of 0.1 ng/L, sensitivity and specificity for immunodiagnosis of patients with angiostrongyliasis by immuno-PCR were 98% (95% confidence interval, 91-99%) and 100% (93-100%), respectively. The test was positive in all parasitologically confirmed cases. Conclusions: Immuno-PCR is a promising technique for diagnosis of A. cantonensis infestation.

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Angiostrongylus(Parastrongylus) and Less Common Nematodes

Yoshimura

2010

Topley &Amp; Wilson's Microbiology and Microbial Infections

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Immunodiagnosis of human eosinophilic meningitis using an antigen of Angiostrongylus cantonensis L5 with molecular weight 204 kD

Cited by 43 publications

References 12 publications

Encephalitis Caused by Angiostrongylus cantonensis after Eating Raw Frogs Mixed with Wine as a Health Supplement

Encephalitis Caused by Angiostrongylus cantonensis after Eating Raw Frogs Mixed with Wine as a Health Supplement

Immuno-PCR for Detection of Antigen to Angiostrongylus cantonensis Circulating Fifth-Stage Worms

Angiostrongylus(Parastrongylus) and Less Common Nematodes

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