Immunodiagnostic/protective role of Cathepsin L cysteine proteinases secreted by Fasciola species

Dixit, A. K.; Dixit, Pooja; Sharma, Rinku

doi:10.1016/j.vetpar.2008.03.017

Cited by 59 publications

(30 citation statements)

References 47 publications

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“…Faecal examination is of little consequence in the host suffering from the acute course of the disease because the onset of adverse effects starts much before appearing of fluke eggs in faeces (Gupta and Yadav 1994;Dixit et al 2008). Quantitative examination of faeces and assessing hepatic fluke load at necropsy are also not sensitive enough to precisely predict severity of the disease (Garg et al 2009).…”

Section: Diagnosismentioning

confidence: 99%

“…Serological demonstration of anti-Fasciola specific antibodies, F. gigantica antigens and immune complexes in host circulation during prepatency appears to be a useful alternate option to coproscopic detection of fluke eggs (Yadav et al 1999a;Dixit et al 2008). Amongst various immunodiagnostic techniques used, ELISA is the most sensitive and acceptable technique but at times suffers from false positive reporting.…”

Section: Diagnosismentioning

confidence: 99%

“…Cross reactivity with other in situ metazoan infections is a major limitation and it prompted a search for specific immuno-dominant moieties in complex somatic and excretory antigens. The role of metabolites released during the development of adolescercariae in conferring acquired immunity in the host, immune evasion and diagnosis of the disease was thoroughly investigated (Dixit et al 2008). Ultimately, Glutathione S-transferase (GST) and Cathepsin L cysteine proteinase (CS) molecules were identified as specific for the purpose.…”

Section: Diagnosismentioning

confidence: 99%

“…Amongst these, the parasite enzymes belonging to cysteine proteinase family secreted by the parasite to help it burrow through various host tissues or cleave the immunoglobulins of the host, have been most intensively studied and gave promising results when used as vaccine antigens (Dixit et al 2008). Dalton et al (1996) in cattle and Edith (2004) in buffalo documented a larger prepatent period (Day 142-143), low mean egg counts, significantly lesser mean wet fluke weight, and lesser mean length and width of the flukes recovered in experimentally immunized with ISAg and ESAg and challenged with the parasites.…”

Section: Control and Immunoprotectionmentioning

confidence: 99%

See 3 more Smart Citations

Epizootiology, pathogenesis and immunoprophylactic trends to control tropical bubaline fasciolosis: an overview

Sharma¹,

Godara²,

Thilagar³

2011

J Parasit Dis

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On the Indian sub-continent, nearly 5,000 years ago, the domestication of the riverine buffalo-the incredible Asian dairy animal was initiated. It plays a versatile role in socio-economic upliftment of its owners from the rural agricultural communities in Asian, African, South American and a few European countries. Comparatively, buffaloes are lesser evolved and susceptible to infectious diseases than cattle. However, poor body thermoregulation and wallowing nature predisposed them to snail borne infections, especially tropical fasciolosis-an incessant major constraint on buffalo production and improvement programmes. This review article is an insight into the global prevalence, varied epizootiological factors, offers possible explanation to pathophysiological clinical signs, deleterious effects of the tropical liver fluke, involving hepato-biliary system, haemopoitic system, endocrine glands and their secretions, oxidative stress, altered metabolism and significant fall in food conversion efficiency with unaffected digestibility of nutrients. Besides, the authors have briefly discussed and reviewed the developments and significance of successful immunodiagnostic approaches for detecting and forecasting the disease during early pre-patency and feasibility of developing a cost effective immunoprotection strategies against tropical fasciolosis.

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Section: Diagnosismentioning

confidence: 99%

Section: Diagnosismentioning

confidence: 99%

Section: Diagnosismentioning

confidence: 99%

Section: Control and Immunoprotectionmentioning

confidence: 99%

See 2 more Smart Citations

Epizootiology, pathogenesis and immunoprophylactic trends to control tropical bubaline fasciolosis: an overview

Sharma¹,

Godara²,

Thilagar³

2011

J Parasit Dis

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“…Several studies pointed out that a 27 kDa protein band is an immunodominant protein band of ES and somatic antigens of Fasciola (Santiago and Hillyer 1988;Attallah et al 2002;Dixit et al 2008;Kamel et al 2013;Intapan et al 1998). However, in few studies, a 29 kDa protein band has been suggested as a principle protein band of Fasciola ES antigen for diagnosis of fasciolosis (Sampaio Silva et al 1996;Hammami et al 1997;Rokni et al 2004).…”

Section: Discussionmentioning

confidence: 99%

Detection of Fasciola hepatica and Fasciola gigantica common and uncommon antigens, using rabbit hyper immune serum raised against their excretory–secretory and somatic antigens

Khabisi

Sarkari

2016

J Parasit Dis

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Fasciolosis is an important neglected helminth disease caused by two liver flukes, Fasciola hepatica and Fasciola gigantica. The two species of Fasciola are usually different in their morphological and molecular features. They have also common and uncommon antigens in both their somatic and excretory secretory metabolites. In this study, we compared somatic and excretory-secretory (ES) antigens of F. hepatica and F. gigantica, by using rabbit hyper immune serum raised against these antigens. Adult worms were collected from bile ducts of infected animals and species of the fluke was confirmed by RFLP-PCR. ES and somatic antigens of both species were prepared. Rabbits were subcutaneously immunized with either ES or somatic antigens to produce antibodies against these antigens. SDS-PAGE pattern of F. hepatica and F. gigantica somatic antigens was similar and both of them revealed 30 protein bands, ranging from 18 to 180 kDa. In contrast, SDS-PAGE pattern of ES antigen of the two species was different. While protein bands with molecular weight of 18, 27, 29, 48, and 62 kDa were common in both species, bands of 19, 45, 55 and 58 kDa were only noticed in F. hepatica ES antigen. Rabbit polyclonal antibodies, raised against F. hepatica and F. gigantica ES antigen, reacted with main five protein bands, 25, 27, 29, 62 and 67 kDa and polyclonal antibodies raised against somatic antigens of both species reacted with three protein bands, 25, 27 and 72 kDa. Thus, the 25, 27 and 29 kDa protein bands may serve as immunodominant antigens, which might be considered for serodiagnosis of fasciolosis. Moreover, bands of 62 and 67 kDa in ES antigen and 72 kDa in somatic antigens of both species were immunodominant and might be suitable candidate for development of serological assays for diagnosis of fasciolosis.

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Water- and Food-Borne Trematodiases in Humans

Khurana¹,

Malla²

2013

Water and Health

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Immunodiagnostic/protective role of Cathepsin L cysteine proteinases secreted by Fasciola species

Cited by 59 publications

References 47 publications

Epizootiology, pathogenesis and immunoprophylactic trends to control tropical bubaline fasciolosis: an overview

Epizootiology, pathogenesis and immunoprophylactic trends to control tropical bubaline fasciolosis: an overview

Detection of Fasciola hepatica and Fasciola gigantica common and uncommon antigens, using rabbit hyper immune serum raised against their excretory–secretory and somatic antigens

Water- and Food-Borne Trematodiases in Humans

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