Immunodominant peptide epitopes of allergen, Asp f 1 from the fungus aspergillus fumigatus

Kurup, Viswanath P.; Banerjee, Banani; Murali, Pazhayannur S.; Greenberger, Paul A.; Krishnan, Meera R.; Hari, V.; Fink, Jordan N.

doi:10.1016/s0196-9781(98)00113-2

Cited by 38 publications

(54 citation statements)

References 43 publications

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“…In a recent study, Svirshchevskaya et al reported that 11 to 23 amino acid residues of Asp f 1 comprise a T-cell epitope (32). However, Kurup et al showed that three peptides from the C terminus of Asp f 1 significantly inhibited IgE binding of Asp f 1 (inhibition ranged from 80 to 100%) (18). In an earlier study, a recombinant N-terminal fragment of Asp f 1 (8 to 45 amino acid residues), which comprised the immunodominant region of the present study, showed binding to specific IgG and IgE antibodies in ABPA patients by immunoblotting and ELISA (29).…”

Section: Discussionmentioning

confidence: 99%

“…Identification of immunodominant regions of Asp f 1 may facilitate the development of specific and standard peptide-based diagnoses. Some of the epitopes of Asp f 1 have been identified by using T-cell clones and peripheral blood mononuclear cells (PBMCs) of A. fumigatus-sensitized patients, but they have not been evaluated for their diagnostic relevance (10,18).…”

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confidence: 99%

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Use of a Synthetic Peptide Epitope of Asp f 1, a Major Allergen or Antigen ofAspergillus fumigatus, for Improved Immunodiagnosis of Allergic Bronchopulmonary Aspergillosis

Madan

Priyadarsiny

Vaid

et al. 2004

Clin Vaccine Immunol

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Allergic bronchopulmonary aspergillosis (ABPA) is an immunologically complex allergic disorder caused by the fungal pathogen Aspergillus fumigatus. Elevated levels of total immunoglobulin E (IgE), specific IgE, and IgG antibodies in sera are important immunodiagnostic criteria for ABPA. International reference standards or standardized immunodiagnostic assays are not available due to a lack of well-defined diagnostic antigens. The present study was carried out to identify and evaluate the immunodiagnostic relevance of synthetic epitopic peptides of Asp f 1, a major allergen, antigen, or cytotoxin of A. fumigatus. Five overlapping peptides were synthesized from the N terminus of Asp f 1, one of the potential immunodominant regions predicted by algorithmic programs. The 11-amino-acid synthetic peptide (P1) significantly inhibited both IgG binding (89.10% ؎ 4.45%) and IgE binding (77.32% ؎ 3.38%) of the standardized diagnostic antigen (SDA) (a well-defined pool of diagnostically relevant allergens and antigens of A. fumigatus). With a panel of sera of ABPA patients, allergic patients with skin test negativity to A. fumigatus, and healthy individuals, P1 showed a higher diagnostic efficiency than SDA (specific IgG, 100%; specific IgE, 98.3%). The diagnostic efficiency of P1 could be attributed to the presence of homologous epitopes in various immunodominant allergens or antigens of A. fumigatus. The ability of P1 to induce histamine release from sensitized mast cells and a Th2 type of cytokine profile in peripheral blood mononuclear cells of ABPA patients suggests its potential for use in intradermal testing. P1 could be further explored for development of a standardized, specific, and sensitive immunodiagnostic test for aspergillosis.

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Section: Discussionmentioning

confidence: 99%

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confidence: 99%

Use of a Synthetic Peptide Epitope of Asp f 1, a Major Allergen or Antigen ofAspergillus fumigatus, for Improved Immunodiagnosis of Allergic Bronchopulmonary Aspergillosis

Madan

Priyadarsiny

Vaid

et al. 2004

Clin Vaccine Immunol

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“…Studies are under way to determine if this extracellular allergen, Asp f 13, plays such a role. Recently, the B-cell epitopes of Aspergillus allergens have been studied [15,16], but information is still incomplete. In the present study, a number of peptides were found to be capable of binding to IgG and IgE antibodies, suggesting strong antigenicity and allergenicity of the molecule, as illustrated schematically in Figure 6.…”

Section: Discussionmentioning

confidence: 99%

“…Many allergenic proteins have already been cloned and expressed, but the characterization of their allergenic determinants has been slow and little is known about IgE-binding epitopes on allergens from A. fumigatus. The C-terminal end of the Asp f 1 allergen has been shown to bind IgE [15] and nine IgE-binding regions have been identified in the hydrophilic region of Asp f 2 [16].…”

Section: Introductionmentioning

confidence: 99%

Identification and expression of an allergen Asp f 13 from Aspergillus fumigatus and epitope mapping using human IgE antibodies and rabbit polyclonal antibodies

Chow¹,

LIU²,

Yu³

et al. 2000

Biochem. J.

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“…Continuous epitopes are short linear sequences of amino acids up to eight residues located and exposed on protein surfaces where the adjacent hydrophilic amino acids contributing to the interaction with antibody (55,56). The identification of sequential epitopes is readily achieved by the systematic synthesis of overlapping short peptides spanning the sequence of the protein allergen.…”

Section: B-cell Epitopesmentioning

confidence: 99%

Molecular biology and immunology of fungal allergens

Kurup

Banerjee

Kelly

et al. 2000

Indian J Clin Biochem

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Fungi are non-chlorophyllus microorganisms, which constitutes the main source of outdoor and indoor allergens. The antigens present in the spores and fragments of hyphae induce allergic responses in sensitized patients. The frequently recognized fungi associated with asthma include Alternaria, Cladosporium, Aspergillus, and Penicillium. With the advent of molecular biology techniques a number of fungal genes encoding relevant allergens have been cloned and the expressed allergens purified and characterized. In this review, we have presented the recent developments, where recombinant allergens have been used in the precise diagnosis of fungal allergy. We have also discussed the role played by these allergens and the T-and B-cell epitopes in the immune mechanism in fungal allergy.

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Immunodominant peptide epitopes of allergen, Asp f 1 from the fungus aspergillus fumigatus

Cited by 38 publications

References 43 publications

Use of a Synthetic Peptide Epitope of Asp f 1, a Major Allergen or Antigen ofAspergillus fumigatus, for Improved Immunodiagnosis of Allergic Bronchopulmonary Aspergillosis

Use of a Synthetic Peptide Epitope of Asp f 1, a Major Allergen or Antigen ofAspergillus fumigatus, for Improved Immunodiagnosis of Allergic Bronchopulmonary Aspergillosis

Identification and expression of an allergen Asp f 13 from Aspergillus fumigatus and epitope mapping using human IgE antibodies and rabbit polyclonal antibodies

Molecular biology and immunology of fungal allergens

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