Immunoelectron microscopic investigation of glutathione-peroxidase in the rat stomach.

Immunocytochemical and Biochemical Studies of Glutathione-Peroxidase(GSH-PO) in Rat Gastric Parietal Cells.

“…Since it is present in such small amounts, it is suggested that mitochondrial GSH-PO is difficult to detect by the ordinary immunoelectron microscopy procedures we have used for immunocytochemistry until now, thus additional studies will be needed to detect this. Murakoshi et al [14] reported that GSH-PO in the rat stomach is localized in parietal cells, associating this localization with the state of acid secretion. Changes in response to histamine and atropine administration by immunoelectron microscopy were then investigated.…”

“…Ultrastructurally, the intracellular membrane system from the intracellular tubular vesicles to the intracellular secretory canaliculus has been found to change dynamically in association with acid secretion [14]. Over the past several years it has been reported that both singlet-oxygen scavenger superoxide dismutase (SOD) and glutathioneperoxidase (GSH-PO), the most effective lipid peroxide scavenger, are present in large amounts in the parietal cells of the gastric mucosa [14,19]. It has been postulated that there is an association between peroxidation and dynamic changes in the intracellular membrane system.…”

Cytoprotective Effect of .ALPHA.-Tocopherol against Parietal Cell Vacuolar Degeneration Induced by Omeprazole in Guinea Pig Gastric Mucosa.

“…Hence, such radical scavengers as superoxide dismutase and glutathione peroxidase are present in the parietal cells to act as a defense system [12,13]. When OPZ is administered, it is specifically taken up by the parietal cells and inhibits gastric acid secretion by blocking the action of H+, K+-ATPase (proton pump) [1,2], an enzyme present in the membranes of the secretory canaliculi.…”