Immunoelectron Microscopic Studies on the Postnatal Development of Gonadotrophs in Rat Anterior Pituitary Glands

Matsubara, Eiji

doi:10.2974/kmj1951.41.365

Cited by 3 publications

(3 citation statements)

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“…The increased small gonadotrophs are supposed to be proliferating cells, since the mitotic frequency in gonadotrophs increases after castration (Inoue et al 1985). The small 5α-reductase-immunoreactive cells that we found 2 weeks after castration may be proliferating and immature gonadotrophs, because they contained poorly developed organelles and a small amount of cytoplasm (Kurosumi et al 1989, Matsubara 1991.…”

Section: Discussionmentioning

confidence: 91%

Steroid 5α-reductase type 1 immunolocalized in the anterior pituitary of intact and castrated male rats

Yokoi

Tsuruo

Miyamoto

et al. 1996

Histochem Cell Biol

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The localization of 5 alpha-reductase was immunohistochemically studied in the anterior pituitary of male rats, using a polyclonal antibody against 5 alpha-reductase rat type 1. The immunoreactive cells were concentrated in the central region and on the border of the intermediate lobe in the anterior pituitary, but not in the intermediate or posterior lobe. The immunoreaction was located mostly in the cytoplasm and occasionally in the cell nuclei. The immunoreactive cells showed alterations in size and number and in the intensity of the immunoreaction after gonadectomy. One week after castration, the cells became larger and the immunoreactivity increased. Two weeks after castration, the number of immunoreactive cells increased. Double immunostaining using anti-luteinizing hormone beta-subunit or anti-follicle stimulating hormone beta-subunit antibody revealed that most of the cells containing 5 alpha-reductase were gonadotrophs. Electron microscopically, the immunoreactive cells showed lamelliform rough endoplasmic reticulum and a depletion of secretory granules 1 week after castration. One week later, the rough endoplasmic reticulum was developed and dilated and the number of secretory granules increased. These results suggest that 5 alpha-reductase is located in the gonadotrophs of rat anterior pituitary and that it is involved in the feedback regulation of gonadotropin secretion by androgens.

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Section: Discussionmentioning

confidence: 91%

Steroid 5α-reductase type 1 immunolocalized in the anterior pituitary of intact and castrated male rats

Yokoi

Tsuruo

Miyamoto

et al. 1996

Histochem Cell Biol

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“…This system of nomenclature was applied to growth hormone (GH) cells and ACTH (POMC) cells ) and also to thyroid-stimulating hormone (TSH) cells by Ozawa and Kurosumi (1989). Gonadotrophs are special and are classified into two major classes by the presence or absence of large granules and are combined with the system of nomenclature according to development, i.e., immature, intermediate, and mature (Matsubara, 1991).…”

Section: Introductionmentioning

confidence: 99%

“…Gonadotrophs are special and are classified into two major classes by the presence or absence of large granules and are combined with the system of nomenclature according to development, i.e., immature, intermediate, and mature (Matsubara, 1991).…”

Section: Introductionmentioning

confidence: 99%

Ultrastructural immunocytochemistry of the adenohypophysis in the rat: A review

Kurosumi

1991

J. Elec. Microsc. Tech.

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Immunocytochemistry has made great strides in the morphology of endocrine glands, especially the adenohypophysis, because the localization of hormones can be clearly demonstrated by this method in the microscopic preparations both for light and electron microscopy. In the adenohypophysis, electron microscopic immunocytochemistry is useful for identifying the producer cell of each hormone. The second contribution is its application to the cell biology of secretion mechanisms. The pituitary hormones, their precursors, derivatives, and fragments were artificially synthesized and their antibodies were produced. Using these antibodies the intracellular sites of synthesis, condensation, processing, and sorting were studied under the electron microscope. The ultrastructure of each cell organelle and its alteration due to the changing function was studied. It was proved that the intracisternal granules in the thyroidectomy cells contain thyroid-stimulating hormone (TSH). The trans-Golgi network or GERL contains a peculiar supporting structure, intracisternal skeleton. Transport of secretory granules may be performed in relation to the microtubules, actin, and some related substances. The most frequently observed mode of hormone release in the adenohypophysis is exocytosis. Sometimes multigranular exocytosis occurs. Vesiculation of membrane around the secretory granules often occur inward or outward. The inward vesiculation forms pinocytotic vesicles, through which the membrane material may be retrieved. The outward vesiculation forms vesicle-like fragments of cytoplasm being discarded to the extracellular space. By these mechanisms the surface area of the cell is maintained constantly.

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Fine structural dynamics showing functional changes of anterior pituitary cells in the rat

Kurosumi

2004

Proceedings of the Japan Academy. Ser. B: Physical and Biologic

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Immunoelectron Microscopic Studies on the Postnatal Development of Gonadotrophs in Rat Anterior Pituitary Glands

Cited by 3 publications

References 20 publications

Steroid 5α-reductase type 1 immunolocalized in the anterior pituitary of intact and castrated male rats

Steroid 5α-reductase type 1 immunolocalized in the anterior pituitary of intact and castrated male rats

Ultrastructural immunocytochemistry of the adenohypophysis in the rat: A review

Fine structural dynamics showing functional changes of anterior pituitary cells in the rat

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