Immunoenzymatic determination of antibody-bound soluble antigens ofTrypanosoma cruzi

Abstract

Anti-Major Cathodic Antigen (MCA) monospecific immunoglobulins ofT. cruzi have been used for the preparation of polyamide-linked immunoadsorbents. A high proportion of serologically positive patients with Chagas disease show the presence of a soluble antigen complexed with human immunoglobulins, which complex binds to those immunoadsorbents as determined by a double sandwich reaction and a peroxidase final determination.

Antigenuria in infants with acute and congenital Chagas' disease

“…Circulating antigens (CAg) have been demonstrated in sera from Trypanosoma cruzi-infected animals (2,12) and human patients (3,22). We have reported detection of specific antigens and immune complexes by enzyme-linked immunosorbent assay (ELISA) in the sera of patients of different ages with acute and chronic Chagas' disease (17).…”